Advertisement

The Role of Intracellular pH in Hormone Action

  • R. D. Moore
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

pH is the measure of the “effective” concentration of protons. In fact, pH is defined in terms of this effective concentration, or thermodynamic activity, of protons: pH ≡ — log αH where αH is the thermodynamic activity of the proton. This definition is especially useful since it means that the pH is directly proportional to the energy, or Gibbs free energy, available from protons to do work. A familiar example provided by this is the fact that the voltage generated by a pH electrode is directly proportional to the pH sensed by that electrode. In a similar manner, in the living cell, pH is directly proportional to the ability of the proton to do work or to affect the cellular machinery.

Keywords

Hormone Action Thermodynamic Activity Epithelial Growth Factor Frog Skeletal Muscle Intracellular Inorganic Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bailey IA, Radda GK, Seymour AL, Williams SR (1982) The effects of insulin on myocardial me-tabolism and acidosis in normoxia and ischaemia. Biochim Biophys Acta 720: 17–27PubMedCrossRefGoogle Scholar
  2. Benos DJ (1982) Amiloride: a molecular probe of sodium transport in tissues and cells. Am J Physiol 242: C131–C145PubMedGoogle Scholar
  3. Brunder DG, Oleynek JJ, Moore RD (1983) In vivo measurement of intracellular pH in soleus muscles of streptozotocin-diabetic rats. J Gen Physiol 82: 154Google Scholar
  4. Buse WB, Nuccitelli R (1984) Metabollic regulation via intracellular pH. Am J Physiol 246:R409–R438Google Scholar
  5. Clancy RL, Gonzalez NC, Shaban M, Cassmeyer V (1983) Acid-base balance in diabetic diaphragms. Meet Fed Proc 42 (3): 477Google Scholar
  6. Clausen T, Kohn PG (1977) The effect of insulin on the transport of sodium and potassium in rat soleus muscle. J Physiol (Lond) 265: 9–42Google Scholar
  7. Fehlmann M, Freychet P (1981) Insulin and glucagon stimulation of (NA+-H+)-ATPase transport activity in isolated rat hepatocytes. J Biol Chem 256: 744–7453Google Scholar
  8. Fidelman ML, Seeholzer SH, Walsh KB, Moore RD (1982) Intracellular pH mediates action of insulin on glycolysis in frog skeletal muscle. Am J Physiol 242: c87–c93PubMedGoogle Scholar
  9. Freiberg JM, Kinsella J, Sacktor B (1982) Glucocorticoids increase the Na+-H+ exchange and decrease the Na+ gradient dependent phosphate-uptake systems in renal brush border membrane vesicles. Proc Natl Acad Sci USA 79: 4932–4936PubMedCrossRefGoogle Scholar
  10. Fenton RA, Gonzalez NC, Clancy RL (1978) The effect of Dibutyryl cyclic AMP and glucagon on the myocardial cell pH. Respir Physiol 32: 213–223PubMedCrossRefGoogle Scholar
  11. Fidelman ML, Seeholzer SH, Walsh KB, Moore RD (1982) Intracellular pH mediates action of insulin on glycolysis in frog Skeletal muscle. Am J Physiol 242: c87–c93PubMedGoogle Scholar
  12. Frieberg JM, Kinsella J, Sacktor B (1982) Glucocorticoids increase the Na+-H+ exchange amd decrease the Na+ gradient dependent phosphate-uptake systems in renal brush border membrane vesicles. Proc Natl Acad Sci USA 79: 4932–4936CrossRefGoogle Scholar
  13. Frelin C, Vigne P, Lazdunski M (1983) The amiloride-sensitive Na+/H+ antiport in 3T3 fibroblasts. J Biol Chem 258: 6272–6276PubMedGoogle Scholar
  14. Funder J, Tosteson DC, Wieth JO (1978) Effects of bicarbonate on lithium transport in human red cells. J Gen Physiol 71: 721–746PubMedCrossRefGoogle Scholar
  15. Funder J, Tosteson DC, Wieth JO (1978) Effects of bicarbonate on lithium transport in human red cells. J Gen Physiol 71: 721–746PubMedCrossRefGoogle Scholar
  16. Karpatkin S, Helmreich E, Cori CF (1964) Regulation of glycolysis in muscle. J Biol Chem 239: 3139–3145PubMedGoogle Scholar
  17. Kinsella J, Cujdik T, Sacktor B (1984) Na+-H+ exchange activity in renal brush border membrane vesicles in response to metabolic acidosis: the rôle of glucocorticoids. Proc Natl Acad Sci USA 81: 630–634PubMedCrossRefGoogle Scholar
  18. Kostyuk PG, Sorokina ZA (1961) In: Kleinzeller A, Kotyk A (eds) Membrane transport metabolism. Academic, New York, pp 193–203Google Scholar
  19. L’Allemain G, Paris S, Pouyssegur J (1984) Growth factor action and intracellular pH regulation in fibroblasts. J Biol Chem 259: 5809–5815PubMedGoogle Scholar
  20. Le Cam A, Auberger P, Sampson M (1982) Insulin enhances protein phosphorylation in isolated hepatocytes by inhibiting an amiloride sensitive phosphotase. Biochem Biophys Res Commun 106: 1062–1070PubMedCrossRefGoogle Scholar
  21. Manchester KL (1970) Speculations on the mechanism of action of insulin. Hormones 1: 342–351PubMedCrossRefGoogle Scholar
  22. Meyer RA, Kushmerick MJ, Dillon PF, Brown (1983) Lack of insulin effect in intracellular pH in mammalian skeletal muscle. Fed Proc 42:al248Google Scholar
  23. Moolenaar WH, Tsien RY, van de Saag PT, de Laat SW (1983) Na+/H+ exchange and cytoplasmic pH in the action of growth factors in human fibroblasts. Nature 304: 645–648Google Scholar
  24. Moore RD (1973) Effect of insulin upon the sodium pump in frog skeletal muscle. J Physiol (Lond) 232: 23–45Google Scholar
  25. Moore RD (1977) Effect of insulin upon intracellular pH. Biophys J 17: 259aGoogle Scholar
  26. Moore RD (1979) Elevation of intracellular pH by insulin in frog skeletal muscle. Biochem Biophys Res Commun 91: 900–904PubMedCrossRefGoogle Scholar
  27. Moore RD, Fidelman ML, Seeholzer SH (1979) Correlation between insulin action upon glycolysis and change in intracellular pH. Biochem Biophys Res Commun 91: 905–910PubMedCrossRefGoogle Scholar
  28. Moore RD (1981) Stimulation of Na:H exchange by insulin. Biophys J 33: 203–210PubMedCrossRefGoogle Scholar
  29. Moore RD (1983) Effects of insulin upon ion transport. Biochem Biophys Acta 737: 1 - 49PubMedGoogle Scholar
  30. Moore RD, Gupta RK (1980) Effect of insulin on intracellular pH as observed by 31P NMR spectroscopy. Int J Quantum Chem, Quantum Biol Symp 7: 83–92Google Scholar
  31. Moore RD, Fidelman ML, Hansen JC, Otis JN (1982) The rôle of intracellular pH in insulin action. In: Nuccitelli R, Deamer DW (eds) Intracellular pH: Its measurement, regulation, and utilization in cellular functions. Alan R. Liss, New York, pp 385–416Google Scholar
  32. Morrill G, Kostellow A, Weinstein SP, Gupta RJ (1983) Hormone induced changes in intracellular Na and pH during the first meiotic division in amphibian oocytes. Fed Proc 42:1791Google Scholar
  33. Podo R, Carpinelli G, D’Agnolo G (1982) International Conference on Magnetic Resonance in Biological Systems, Stanford, Aug 29-Sept 3, p 14 Google Scholar
  34. Pouyssegur J, Chambard JC, Paris S (1982) In: Boynton AL, McKeehan WL, Whitfield JF (eds) Symposium on ions, cell proliferation and cancer. Academic, New York, pp 205-218Google Scholar
  35. Putnam RW, Roos A (1983) Insulin effects on pHi and Vm of frog muscle. Physiologist 26(4):A70 Rasmussen HGoogle Scholar
  36. Rothenberg P, Glasen L, Schlesinger P, Cassel D (1983) Aetivation of Na+/H* exchange by epidermal growth factor elevates intracellular pH in A431 cells. J Biol Chem 258: 12644–12653PubMedGoogle Scholar
  37. Skou JC (1982) The [Na++K+]-ATPase: Coupling of the reaction with ATP to the reaction with Na+ and K+. Ann NY Acad Sci 402 (II): 169 - 184PubMedCrossRefGoogle Scholar
  38. Sonne O, Gliemann J, Linde S (1981) Effect of pH on binding kinetics and biological effect of insulin in rat adipocytes. J Biol Chem 256: 6250–6254PubMedGoogle Scholar
  39. Straus DS (1981) Effects of insulin on cellular growth and proliferation. Life Sci 29: 2131–2139PubMedCrossRefGoogle Scholar
  40. Trivedi B, Danforth WH (1966) Effect of pH on the kinetics of frog muscle fructokinase. J Biol Chem 241: 4110–4114PubMedGoogle Scholar
  41. Winkler MM (1982) Regulation of protein synthesis in sea urchin eggs by intracellular pH. In: Nuccitelli R, Deamer DW (eds) Intracellular pH: Its measurement, regulation, and utilization in cellular functions. Alan R. Liss, New York, pp 325–340Google Scholar
  42. Winkler MM, Steinhardt RA (1981) Aetivation of protein synthesis in a sea urchin cell-free system. Dev Biol 84: 432–443PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • R. D. Moore
    • 1
    • 2
  1. 1.Biophysics LaboratoryState University of New YorkPlattsburghUSA
  2. 2.Dept. of Physiology and Biophysics, College of MedicineUniversity of VermontBurlingtonUSA

Personalised recommendations