The Relationship Between Erythrocyte Phosphate Metabolism, Carbon Dioxide, and pH on Blood Oxygen Affinity in Birds

  • R. E. Isaacks
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


In recent years, we have become increasingly aware of the remarkable diversity in the different organic phosphate compounds in erythrocytes, many of which have been shown to modulate hemoglobin oxygenation. For example: 2,3-bisphosphoglycerate (2,3-P2-glycerate) previously considered a characteristic only of mammalian erythrocytes, has been observed as a major constituent of the red cells of embryos of birds and reptiles and in the red cells of the armored catfish (Borgese and Lampert 1975; Isaacks and Harkness 1975, 1980); inositol tetrakisphosphate (inositol-P4) was found to be the major organic phosphate in erythrocytes of the mature ostrich (Isaacks and Harkness 1980); inositol pentakisphosphate (inositol-P5) previously considered a characteristic of avian erythrocytes has been observed in erythrocytes of several species of fishes and sea turtles (Bartlett 1980; Borgese and Nagel 1978; Isaacks and Harkness 1980; Rapoport and Guest 1941); inositol bisphosphate (inositol-P2) has been found in erythrocytes of the South American (Lepidosiren paradoxa) and African (Protopterus aethiopicus) lungfish (Bartlett 1980; Isaacks and Harkness 1980), but its role as a modifier of hemoglobin function has not been verified; and in some of our very recent studies the red blood cells of monotremes, the egg-laying mammals (echidna and duckbill platypus), were found nearly devoid of adenosine triphosphate (ATP) (Kim et al. 1981; Isaacks et al. 1984), only traces were present (0.03 and 0.06 mM, respectively).


Organic Phosphate Erythrocyte Suspension Young Chick Bohr Effect Adult Chicken 
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  1. Arnone A,Perutz MF (1974) Structure of inositol hexaphosphate-human deoxyhaemoglobin complex. Nature 249:34–36PubMedCrossRefGoogle Scholar
  2. Bartlett GR (1980) Phosphate compounds in vertebrate red blood cells. Am Zool 20: 103–114Google Scholar
  3. Bauer C (1974) On the respiratory function of haemoglobin. Rev Physiol Biochem Pharmacol 70: 1–31PubMedCrossRefGoogle Scholar
  4. Bauer C, Jelkman W (1977) Carbon dioxide governs the oxygen affinity of crocodile blood. Nature 269: 825–827PubMedCrossRefGoogle Scholar
  5. Belkin DA (1963) Anoxia: Tolerance in reptiles. Science 139: 492–493PubMedCrossRefGoogle Scholar
  6. Benesch R, Benesch RE (1967) The effect of organic phosphates from the human erythrocyte on the allosteric properties of hemoglobin. Biochem Biophys Res Commun 26: 162–167PubMedCrossRefGoogle Scholar
  7. Benesch R, Benesch RE, Yu CI (1968) Reciprocal binding of O2 and diphosphoglycerate by human hemoglobin. Proc Natl Acad Sci USA 59: 526–532PubMedCrossRefGoogle Scholar
  8. Bentley TB, Lutz PL (1979) Diving anoxia and nitrogen breathing anoxia in the marine logger- head turtle. Am Zool 19 (3): 982Google Scholar
  9. Borgese TA, Lampert LM (1975) Duck red cell 2, 3-diphosphoglycerate: Its presence in the embryo and its disappearance in the adult. Biochem Biophys Res Commun 65: 822–827Google Scholar
  10. Borgese TA, Nagel RL (1978) Inositol pentaphosphate in fish red blood cells. J Exp Zool 205: 133–140PubMedCrossRefGoogle Scholar
  11. Burger RE (1980) Respiratory gas exchange and control in the chicken. Poult Sci 59: 2654–2665PubMedGoogle Scholar
  12. Calder WA, Schmidt-Nielsen K (1968) Panting and blood carbon dioxide in birds. Am J Physiol 215: 477–482PubMedGoogle Scholar
  13. Chanutin A, Curnish RR (1967) Effect of organic and inorganic phosphates on the oxygen equi- librium of human erythrocytes. Arch Biochem Biophys 121: 96–102PubMedCrossRefGoogle Scholar
  14. Clausen G, Sanson R, Storesund A (1971) The HbO2 dissociation curve of the fulmar and the herring gull. Respir Physiol 12: 66–70PubMedCrossRefGoogle Scholar
  15. Danzer LA, Cohn JE (1967) The dissociation curve for goose blood. Respir Physiol 3: 302 — 306PubMedCrossRefGoogle Scholar
  16. Fedde MR (1980) Structure and gas-flow pattern in the avian respiratory system. Poult Sci 59: 2642–2653PubMedGoogle Scholar
  17. Felger RS, Cliffton K, REgal PJ (1976) Winter dormancy in Sea Turtles: Independent discovery and exploitation in the Gulf of California by two local cultures. Science 191: 283–285Google Scholar
  18. Grigg GC, Gruca M (1979) Possible adaptive significance of low red cell organic phosphates in crocodiles. J Exp Zool 209 (1): 161–167CrossRefGoogle Scholar
  19. Grigg GC, Gruca M (1979) Possible adaptive significance of low red cell organic phosphates in crocodiles. J Exp Zool 209 (1): 161–167CrossRefGoogle Scholar
  20. Isaacks RE, Harkness DR (1980) Erythrocyte organic phosphates and hemoglobin function in birds, reptiles, and fishes. Am Zool 20: 115–129Google Scholar
  21. Isaacks RE, Harkness DR, White JR (1982a) Regulation of hemoglobin function and whole blood oxygen affinity by carbon dioxide and pH in the Loggerhead (Caretta caretta) and green sea turtle (Chelonia mydas mydas). Hemoglobin 6 (6): 549–568PubMedCrossRefGoogle Scholar
  22. Isaacks RE, Kim CY, Johnson AE Jr, Goldman PH, Harkness DR (1982b) Studies on avian erythrocyte metabolism. XII. The synthesis and degradation of inositol pentakis (dihydrogen phosphate). Poult Sci 61: 2271–2281Google Scholar
  23. Isaacks RE, Kim CY, Liu HL, Goldman PH, Johnson AE Jr, Harkness DR (1983) Studies on avian erythrocyte metabolism. XIII. Changing organic phosphate composition in age-dependent density populations of chicken erythrocytes. Poult Sci 62: 1639–1646Google Scholar
  24. Isaacks RE, Nicols SC, Sallis JD, Zeidler RB, Kim HD (1984) Erythrocyte phosphates and hemoglobin function in monotremes and some marsupials. Am J Physiol 246: R236–R241PubMedGoogle Scholar
  25. Isaacks RE, Goldman PH, Kim CY, Harkness DR (1985) Studies in avian erythrocyte metabolism. XIV. Effectof CO2 and PH on P50 in the chicken. Am J Physiol (in press)Google Scholar
  26. Jones SR, Smith J, Board PB (1978) Changes in erythrocyte metabolism following acute blood loss in chickens. Poult Sci 57: 1667–1674Google Scholar
  27. Kim HD, Zeidler RB, Sallis JD, Nicol SC, Isaacks RE (1981) Adenosine triphosphate-deficient erythrocytes of the egg-laying mammal, echidna ( Tachyglossus aculeatus ). Science 213: 1517–1519Google Scholar
  28. Lian CY, Roth S, Harkness DR (1971) The effect of alteration of intracellular 2,3-DPG concentration upon oxygen binding of intact erythrocytes containing normal and mutant hemoglobins. Biochem Biophys Res Commun 45: 151–158PubMedCrossRefGoogle Scholar
  29. Longmuir IS, Chow J (1970) Rapid method for determining effect of agents on oxyhemoglobin dissociation curves. J Appl Physiol 28: 343 — 345PubMedGoogle Scholar
  30. Matsuda G, Maita T, Mizuno K, Ota H (1973) Amino acid sequence of a beta-chain of AII component of adult chicken haemoglobin. Nature [New Biol] 244: 244Google Scholar
  31. Millen JR, Murdaughjun HV, Baver CB, Robin ED (1964) Circulatory adaptation to diving in the freshwater turtle. Science 145: 591–593PubMedCrossRefGoogle Scholar
  32. Rapoport S, Guest GM (1941) Distribution of acid-soluble phosphorus in blood cells of various vertebrates. J Biol Chem 138: 269–282Google Scholar
  33. Rose ZB (1976) A procedure for decreasing the level of 2,3-biphosphoglycerate in red cells in vitro. Biochem Biophys Res Commun 73: 1011–1017PubMedCrossRefGoogle Scholar
  34. Vandecasserie C, Paul C, Schnek AG et al. (1973) Oxygen affinity of avian hemoglobins. Comp Biochem Physiol 44A:711–718CrossRefGoogle Scholar
  35. Wells RMC (1976) The oxygen affinity of chicken hemoglobin in whole blood and erythrocyte suspensions. Respir Physiol 27:21–31CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • R. E. Isaacks
    • 1
    • 2
  1. 1.Research LaboratoriesVeterans Administration Medical CenterMiamiUSA
  2. 2.Department of MedicineUniversity of MiamiMiamiUSA

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