DNA-Poly(ADP-Ribose) Polymerase Complex: Isolation of the DNA Wrapping the Enzyme Molecule

  • Marie E. Ittel
  • Jenny Jongstra-Bilen
  • Claude Niedergang
  • Paul Mandel
  • Etienne Delain
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Since the discovery of poly(ADP-ribose) it has been known that DNA is required for the synthesis of the polymer from NAD+ [1, 2]. It was confirmed later with purified poly(ADP-ribose) polymerase that double-stranded DNA is necessary to express its activity in vitro [3–7], Covalently-closed circular double-stranded DNA does not have any effect on the enzyme activity [7, 8]. It has been found by Ohgushi et al. [8] that the relative efficiency of enzyme activation by various DNA samples is closely related to their capacity to bind to the enzyme. These authors have suggested that poly(ADP-ribose) polymerase is activated by, and binds to, nicks or ends on the DNA. The highly purified calf thymus poly(ADP-ribose) polymerase preparation obtained in our laboratory [9, 10] contains a fraction of DNA, called sDNA, which has been shown to activate the enzyme more efficiently than total calf thymus DNA [6]. A DNA fraction called “active DNA” with high enzyme binding and activating capacity has also been isolated during the purification of bovine thymus poly(ADP-ribose) polymerase by Yoshihara and co-workers [5, 11]. The high affinity of this enzyme for the “active DNA” has been attributed to its small size and its high content of nicks [8].

Keywords

Migration Phenol Agarose Bromide Adenosine 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Marie E. Ittel
    • 1
  • Jenny Jongstra-Bilen
    • 2
  • Claude Niedergang
    • 1
  • Paul Mandel
    • 1
  • Etienne Delain
    • 3
  1. 1.Centre de Neurochimie du CNRSStrasbourg CedexFrance
  2. 2.Dept. of Neurobiology, School of MedicineStanford UniversityStanfordUSA
  3. 3.Laboratoire de Microscopie Cellulaire et Moléculaire, LA 147 du CNRSInstitut Gustave RoussyVillejuifFrance

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