Inhibitors of Poly(ADP-Ribose) Polymerase Prevent Friend Cell Differentiation

  • Tim Brac
  • Kaney Ebisuzaki
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Friend et al. [1] made the striking observation that dimethyl sulfoxide (DMSO)-treated Friend erythroleukemia cells (FEL) differentiate in vitro. Subsequently, a number of other chemicals, including butyric acid, hypoxanthine and hexamethylene bis-acetamide were shown to induce Friend cells [2]. These inducers appear to remove a block in the differentiation process but the mechanisms involved are unknown. The finding that benzamide and nicotinamide induced Friend cells [3, 4] suggested that since both compounds were inhibitors of poly(ADP-ribose) polymerase, poly(ADP-ribosylation) may have a role in the differentiation process. Furthermore, since poly(ADP-ribose) polymerase requires DNA strand breaks for activity [5], these observations implicated DNA strand breaks in FEL differentiation.


Strand Break Sodium Butyrate Erythroid Differentiation Dimethyl Sulfate Alkaline Elution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Tim Brac
  • Kaney Ebisuzaki
    • 1
  1. 1.Cancer Research LaboratoryUniversity of Western OntarioLondonCanada

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