Inhibitors of Poly(ADP-Ribose) Polymerase Prevent Friend Cell Differentiation
Friend et al.  made the striking observation that dimethyl sulfoxide (DMSO)-treated Friend erythroleukemia cells (FEL) differentiate in vitro. Subsequently, a number of other chemicals, including butyric acid, hypoxanthine and hexamethylene bis-acetamide were shown to induce Friend cells . These inducers appear to remove a block in the differentiation process but the mechanisms involved are unknown. The finding that benzamide and nicotinamide induced Friend cells [3, 4] suggested that since both compounds were inhibitors of poly(ADP-ribose) polymerase, poly(ADP-ribosylation) may have a role in the differentiation process. Furthermore, since poly(ADP-ribose) polymerase requires DNA strand breaks for activity , these observations implicated DNA strand breaks in FEL differentiation.
KeywordsStrand Break Sodium Butyrate Erythroid Differentiation Dimethyl Sulfate Alkaline Elution
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