Inhibitors of Poly(ADP-Ribose) Polymerase Prevent Friend Cell Differentiation
Friend et al.  made the striking observation that dimethyl sulfoxide (DMSO)-treated Friend erythroleukemia cells (FEL) differentiate in vitro. Subsequently, a number of other chemicals, including butyric acid, hypoxanthine and hexamethylene bis-acetamide were shown to induce Friend cells . These inducers appear to remove a block in the differentiation process but the mechanisms involved are unknown. The finding that benzamide and nicotinamide induced Friend cells [3, 4] suggested that since both compounds were inhibitors of poly(ADP-ribose) polymerase, poly(ADP-ribosylation) may have a role in the differentiation process. Furthermore, since poly(ADP-ribose) polymerase requires DNA strand breaks for activity , these observations implicated DNA strand breaks in FEL differentiation.
KeywordsDMSO Dimethyl Catalase Pyrimidine Thymidine
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- 9.Shall S (1982) ADP-ribose in DNA repair. In: Hayaishi O, Ueda K (eds) ADP-ribosylation reactions in biology and medicine. Academic Press, London New York, p 478Google Scholar
- 16.Gopalakrishnan TV, French Anderson W (1979) Mouse erythroleukemia cells. In: Jakoby WB, Pastan IH (eds) Cell culture. Methods in enzymology, vol 58. Academic Press, London New York, pp 506–511Google Scholar
- 18.Kohn KW, Ewig RAG, Erickson LC, Zwelling LA (1981) Measurements of strand breaks and crosslinks in DNA by alkaline elution. In: Friedberg E, Hanawalt P (eds) DNA repair: a laboratory manual of research procedures. Marcel Dekker, New York, p 379Google Scholar
- 23.Tanuma S, Johnston LD, Johnston GS (1983) ADP-ribosylation of chromosomal proteins and mouse mammary tumor virus gene expression. J Biol Chem 258:15731–15735Google Scholar