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Effects of 3-Aminobenzamide on Cell-Cycle Traverse and Viability of Human Cells Exposed to Agents which Induce DNA Strand-Breakage

  • Paul J. Smith
  • Catherine O. Anderson
  • Steven H. Chambers
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

There is evidence that poly(ADP-ribosyl)ation, in response to DNA damage (review [1]), permits changes in chromatin structure [2] which may facilitate the activity of repair enzymes [3] and become a major factor in the control of cell-cycle traverse [4]. This paper explores the role of chromatin structure and poly(ADP-ribosyl)ation (using the inhibitor, 3-aminobenzamide; 3AB; [5]) in the responses of human cells to either a DNA specific ligand (Hoechst dye 33341; Ho33342) or X-radiation. Cells derived from an ataxia telangiectasia (A-T) patient have been included in the study to provide a hypersensitive control (reviews [6, 7]) in which anomalous cell survival and cell-cycle responses [8–10] to radiation may reflect a primary defect in chromatin structure [11, 12].

Keywords

HT29 Cell Ataxia Telangiectasia Ataxia Telangiectasia HT29 Cell Line Fibroblast Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Paul J. Smith
  • Catherine O. Anderson
  • Steven H. Chambers
    • 1
  1. 1.MRC Clinical Oncology and Radiotherapeutics UnitCambridgeGreat Britain

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