Effects of 3-Aminobenzamide on Cell-Cycle Traverse and Viability of Human Cells Exposed to Agents which Induce DNA Strand-Breakage

  • Paul J. Smith
  • Catherine O. Anderson
  • Steven H. Chambers
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


There is evidence that poly(ADP-ribosyl)ation, in response to DNA damage (review [1]), permits changes in chromatin structure [2] which may facilitate the activity of repair enzymes [3] and become a major factor in the control of cell-cycle traverse [4]. This paper explores the role of chromatin structure and poly(ADP-ribosyl)ation (using the inhibitor, 3-aminobenzamide; 3AB; [5]) in the responses of human cells to either a DNA specific ligand (Hoechst dye 33341; Ho33342) or X-radiation. Cells derived from an ataxia telangiectasia (A-T) patient have been included in the study to provide a hypersensitive control (reviews [6, 7]) in which anomalous cell survival and cell-cycle responses [8–10] to radiation may reflect a primary defect in chromatin structure [11, 12].


HT29 Cell Ataxia Telangiectasia Ataxia Telangiectasia HT29 Cell Line Fibroblast Line 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Paul J. Smith
  • Catherine O. Anderson
  • Steven H. Chambers
    • 1
  1. 1.MRC Clinical Oncology and Radiotherapeutics UnitCambridgeGreat Britain

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