Effects of 3-Aminobenzamide on Cell-Cycle Traverse and Viability of Human Cells Exposed to Agents which Induce DNA Strand-Breakage
There is evidence that poly(ADP-ribosyl)ation, in response to DNA damage (review ), permits changes in chromatin structure  which may facilitate the activity of repair enzymes  and become a major factor in the control of cell-cycle traverse . This paper explores the role of chromatin structure and poly(ADP-ribosyl)ation (using the inhibitor, 3-aminobenzamide; 3AB; ) in the responses of human cells to either a DNA specific ligand (Hoechst dye 33341; Ho33342) or X-radiation. Cells derived from an ataxia telangiectasia (A-T) patient have been included in the study to provide a hypersensitive control (reviews [6, 7]) in which anomalous cell survival and cell-cycle responses [8–10] to radiation may reflect a primary defect in chromatin structure [11, 12].
KeywordsHT29 Cell Ataxia Telangiectasia Ataxia Telangiectasia HT29 Cell Line Fibroblast Line
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- 1.Shall S (1982) ADP-ribose in DNA repair. In: Hayaishi O, Ueda K (eds) ADP-ribosylation reactions. Academic Press, London New York, p 472Google Scholar
- 7.Bridges BA, Harnden DG (1982) Ataxia-telangiectasia — a cellular and molecular link between cancer, neuropathology and immune deficiency. Wiley, ChichesterGoogle Scholar
- 14.Darzynkiewicz Z (1979) Acridine orange as a molecular probe in studies of nucleic acids in situ. In: Melamed MR, Mullaney PF, Mendelsohn ML (eds) Flow cytometry and sorting. Wiley, New York, p 285Google Scholar
- 15.Latt SA (1979) Fluorescent probes of DNA microstructure and synthesis. In: Melamed MR, Mullaney PF, Mendelsohn ML (eds) Flow cytometry and sorting. Wiley, New York, p 139Google Scholar
- 19.Ganesan AK, Smith CA, Van Zeeland AA (1981) Measurement of the pyrimidine dimer content in DNA in permeabilized bacterial or mammalian cells with endonuclease V of bacteriophage T4. In: Friedberg EC, Hanawalt PC (eds) DNA repair. A laboratory manual of research techniques, vol I. Marcel Dekker, New York, p 89Google Scholar