Mono(ADP-Ribosyl) Histone H1 in Alkylated Hepatoma Cells: Unusual Acceptor Site

  • Andreas Kreimeyer
  • Helmuth Hilz
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Treatment of cells with DNA-damaging agents like UV irradiation or alkylating chemicals is known to greatly stimulate mono- and poly(ADPR) formation [1, 2] and turnover [3, 4]. Although there are many attempts to elucidate the function of poly(ADP-ribosyl)ation in DNA repair, the role of cellular mono(ADP-ribosyl)ation in the course of DNA damage has received relatively little attention. Recently Kreimeyer et al. [5] were able to demonstrate that histones were extensively mono(ADP-ribosyl)ated in intact DMS-treated hepatoma AH7974 cells. 20–30% of DMS-induced mono(ADPR) was found to be associated with histone H1 and nearly 60% with core histones. Adamietz et al. [6] identified histone H2B as the main acceptor of mono(ADPR) under these conditions. Surprisingly, mono(ADPR)-Hl as well as mono(ADPR)-H2B conjugates seemed to be resistant towards neutral hydroxylamine, indicating a linkage between ADPR and histone that was different from the ester glycosidic bond observed with poly(ADPR) in liver nuclei [5].

Keywords

Carboxyl Electrophoresis Adduct Polypeptide Arginine 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Andreas Kreimeyer
  • Helmuth Hilz
    • 1
  1. 1.Institut für Physiologische Chemie der Universität HamburgHamburg 20Germany

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