Cytoplasmic Poly(ADP-Ribose) Synthetase in Rat Spermatogenic Cells

  • Ilona I. Concha
  • Margarita I. Concha
  • Jaime Figueroa
  • Luis O. Burzio
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Poly(ADP-ribose) synthetase uses NAD as a substrate to catalyze the formation of a homopolymer of repeating ADP-ribose units [1–3]. The oligomers and polymers of ADP-ribose are synthesized in a covalent association with various proteins of which histones H1 and H2B [4–7] and the synthetase itself [8–10] are the best characterized.


Seminiferous Tubule Microsomal Fraction Synthetase Activity Spermatogenic Cell Ehrlich Ascites Tumor Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Hilz H, Stone PR (1976) Poly(ADP-ribose) and ADP-ribosylation of protein. In: Lynen F (ed) Physiol Biochem Pharmacol, vol 76. Springer, Berlin Heidelberg New York, pp 1–58CrossRefGoogle Scholar
  2. 2.
    Hayaishi O, Ueda K (1977) Poly(ADP-ribose) and ADP-ribosylation of proteins. Annu Rev Biochem 46:95–116PubMedCrossRefGoogle Scholar
  3. 3.
    Sugimura T, Miwa M (1982) Structure and properties of poly(ADP-ribose). In: Hayaishi O, Ueda K (eds) ADP-ribosylation reactions. Academic Press, London New York, pp 43–63Google Scholar
  4. 4.
    Riquelme PT, Burzio LO, Koide SS (1979) ADP-ribosylation of rat liver lysine-rich histone in vitro. J Biol Chem 254:3018–3028PubMedGoogle Scholar
  5. 5.
    Burzio LO, Riquelme PT, Koide SS (1979) ADP-ribosylation of rat liver nucleosomal core histones. J Biol Chem 254:3029–3037PubMedGoogle Scholar
  6. 6.
    Ogata N, Ueda K, Kagamiyama H, Hayaishi O (1980) ADP-ribosylation of histone H1. J Biol Chem 255:7616–7620PubMedGoogle Scholar
  7. 7.
    Ogata N, Ueda K, Hayaishi O (1980) ADP-ribosylation of histone H2B, J Bio1 Chem 255: 7610–7615Google Scholar
  8. 8.
    Yoshihara K, Hashida T, Yoshihara H, Tanaka Y, Ohgushi H (1977) Enzyme-bound early product of purified poly(ADP-ribose) polymerase. Biochem Biophys Res Commun 78:1281 – 1288PubMedCrossRefGoogle Scholar
  9. 9.
    Kawaichi M, Ueda K, Hayaishi O (1981) Multiple autopoly(ADP-ribosylation) of rat liver poly(ADP-ribose) synthetase: Mode of modification and properties of automodified synthetase. J Biol Chem 256:9483–9489PubMedGoogle Scholar
  10. 10.
    Mandel P, Jongstra-Bilen J, Ittel ME, de Murcia G, Delain E, Niedergang C, Vosberg HP (1983) Some electron microscopy ascpects of poly(ADPR) polymerase-DNA interaction and of auto-poly(ADP-ribosyl)ation reaction. In: Miwa M, Hayaishi O, Shall S, Smulson M, Sugimura T (eds) ADP-ribosylation, DNA repair and cancer. Jpn Sci Soc Pross, Tokyo, pp 71–81Google Scholar
  11. 11.
    Burzio LO, Reich L, Koide SS (1975) Poly(adenosine diphosphoribose) synthase activity of isolated nuclei of normal and leukemic leukocytes. Proc Soc Exp Biol Med 149:933–938PubMedGoogle Scholar
  12. 12.
    Whitby AJ, Stone PR, Whish WJ (1979) Effect of polyamines and Mg2+ on poly(ADP-ribose) synthesis and ADP-ribosylation of histones in wheat. Biochem Biophys Res Commun 90: 1295–1304PubMedCrossRefGoogle Scholar
  13. 13.
    Roberts JH, Stark P, Giri C, Smulson M (1975) Cytoplasmic poly(ADP-ribose) polymerase during the HeLa cells cycle. Arch Biochem Biphys 171:305–315CrossRefGoogle Scholar
  14. 14.
    Kun E, Zimber PH, Chang AC, Puschendorf B, Grunicke H (1975) Macromolecular enzymatic product of NAD in liver mitochondria. Proc Natl Acad Sci USA 72:1436–1440PubMedCrossRefGoogle Scholar
  15. 15.
    Burzio LO, Sáez L, Cornejo R (1981) Poly(ADP-ribose) synthetase activity in rat testis mitochondria. Biochem Biophys Res Commun 103:369–375PubMedCrossRefGoogle Scholar
  16. 16.
    Burzio LO, Concha II, Figueroa J, Concha M (1983) Poly(ADP-ribose) synthetase associated to cytoplasmic structure of meiotic cells. In: Miwa M, Hayaishi O, Shall S, Smulson M, Sugimura T (eds) ADP-ribosylation, DNA repair and cancer. Jpn Sci Soc Press, Tokyo, pp 141–152Google Scholar
  17. 17.
    Noll H (1969) Polysomes: Analysis of structure and function. In: Campbell PN, Sargent JR (eds) Techniques in protein biosynthesis, vol II. Academic Press, London New York, pp 101–179Google Scholar
  18. 18.
    Shima T, Hasegawa S, Fujimura S, Matsubara H, Sugimura T (1969) Studies on poly(adenosine diphosphate-ribose). VII. Methods of separation and identification of 2′-(5″-phosphoribosyl)-5′-adenosine monophosphate. J Biol Chem 244:6632–6635PubMedGoogle Scholar
  19. 19.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  20. 20.
    Yamada M, Miwa M, Sugimura T (1971) Studies on poly(adenosine diphosphate-ribose). X. Properties of a partially purified poly (adenosine diphosphate-ribose) polymerase. Arch Biochem Biophys 146:579–586PubMedCrossRefGoogle Scholar
  21. 21.
    Ohgushi H, Yoshihara K, Kamiya T (1980) Bovine thymus poly(adenosine diphosphate-ribose) polymerase. Physical properties and binding to DNA. J Biol Chem 255:6205–6211PubMedGoogle Scholar
  22. 22.
    Kristensen T, Holtlund J (1976) Purification of poly(ADP-ribose) polymerase from Ehrlich ascites tumor cells by chromatography on DNA-agarose. Eur J Biochem 70:441–446PubMedCrossRefGoogle Scholar
  23. 23.
    Benjamin RC, Gill M (1980) A connection between poly(ADP-ribose) synthesis and DNA strand breakage. In: Smulson ME, Sugimura T (eds) Novel ADP-ribosylations of regulatory enzymes and proteins. Elsevier/North Holland, Amsterdam New York, pp 227–236Google Scholar
  24. 24.
    Ueda K, Kawaichi M, Hayaishi O (1982) Poly(ADP-ribose) synthetase. In: Hayaishi O, Ueda K (eds) ADP-ribosylation reactions. Academic Press, London New York, pp 117–155Google Scholar
  25. 25.
    Price PA (1975) The essential role of Ca2+ in the activity of bovine pancreatic deoxyribonuclease. J Biol Chem 250:1981–1986PubMedGoogle Scholar
  26. 26.
    Clermont Y, Perey B (1955) Quantitative study of the cell population of the seminiferous tubules in immature rats. Annu J Anat 100:241–268Google Scholar
  27. 27.
    Momii A, Koide SS (1980) RNA synthesis and poly(ADP-ribose) synthetase activity in developing mouse testis. Fed Proc 39:954Google Scholar
  28. 28.
    Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody-enzyme method of immunohistochemistry. Preparation and properties of antigenantibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–321PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Ilona I. Concha
    • 1
  • Margarita I. Concha
    • 1
  • Jaime Figueroa
    • 1
  • Luis O. Burzio
    • 1
  1. 1.Instituto de Bioquímica, Facultad de CienciasUniversidad Austral de ChileValdiviaChile

Personalised recommendations