Abstract
We have devised an activity gel procedure to recover the functional poly(ADP-ribose) polymerase catalytic activities after SDS-polyacrylamide gel electrophoresis [1]. Using this technique, we analyzed the catalytic polypeptides of this enzyme present in extracts obtained from a variety of organisms, belonging to a wide evolutionary scale. We also measured the enzyme in human lymphocytes before and after stimulation with mitogens and in human cells of different leukemia states.
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References
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© 1985 Springer-Verlag Berlin Heidelberg
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Scovassi, A.I., Franchi, E., Isernia, P., Brusamolino, E., Stefanini, M., Bertazzoni, U. (1985). Activity Gels of Poly(ADP-Ribose) Polymerase: Phylogenetic Studies and Variations in Human Blood Cells. In: Althaus, F.R., Hilz, H., Shall, S. (eds) ADP-Ribosylation of Proteins. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70589-2_15
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DOI: https://doi.org/10.1007/978-3-642-70589-2_15
Publisher Name: Springer, Berlin, Heidelberg
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