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Kinetic Mechanism of Poly(ADP-Ribose) Polymerase

  • Robert C. Benjamin
  • Paul F. Cook
  • Myron K. Jacobson
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Poly(ADP-ribose) polymerase is a chromatin-associated enzyme which, in the presence of fragmented DNA, assembles branched homopolymers from the ADP-ribose moiety of NAD (reviewed in [1]). Fragmented DNA is an essential activator of the polymerase and is not modified by the reaction. This report presents data related to the kinetic mechanism of DNA activation of the poly(ADP-ribose) polymerase.

Keywords

Enzyme Kinetic Data Similar Kinetic Property Siliconized Glass Tube EDTA Ethylenediamine Tetraacetic Acid Initial Velocity Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

poly(dT)

poly(deoxythymidylic acid)

EDTA

ethylenediamine tetraacetic acid

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References

  1. 1.
    Pekala PH, Moss J (1983) Poly(ADP-ribosylation) of protein. Cur Top Cell Regul 22:1–49Google Scholar
  2. 2.
    Benjamin RC, Gill DM (1980) Poly(ADP-ribose) synthesis in vitro programmed by damaged DNA: A comparison of DNA molecules containing different types of strand breaks. J Biol Chem 255:10502–10508PubMedGoogle Scholar
  3. 3.
    Niedergang C, Okazaki H, Mandel P (1979) Properties of purified calf thymus poly(ADP-ribose) polymerase. Eur J Biochem 102:43–57PubMedCrossRefGoogle Scholar
  4. 4.
    Cleland WW (1963) Statistical analysis of enzyme kinetic data. Methods Enzymol 63:103–138CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Robert C. Benjamin
    • 1
  • Paul F. Cook
    • 1
  • Myron K. Jacobson
    • 1
  1. 1.Department of Biochemistry, Texas College of Osteopathic MedicineNorth Texas State UniversityDentonUSA

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