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Surface Components of Escherichia coli That Mediate Resistance to the Bactericidal Activities of Serum and Phagocytes

  • Chapter
Genetic Approaches to Microbial Pathogenicity

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 118))

Abstract

Microorganisms that enter the animal body, either actively or passively, encounter a battery of nonspecific and specific chemical and cellular defenses whose role is to inactivate and eliminate foreign materials. Successful pathogens have the ability to avoid, resist, or inactivate these defenses for at least the period necessary for initiation of the disease process.

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References

  • Achtman M, Kennedy N, Skurray R (1977) Cell-cell interactions in conjugating Escherichia coli: role of traT protein in surface exclusion. Proc Natl Acad Sci USA 74:5704–5708

    Article  Google Scholar 

  • Agüero MA, Cabello FC (1983) Relative contribution of ColV plasmid and Kl antigen to the pathogenicity of Escherichia coli. Infect Immun 40:359–368

    PubMed  Google Scholar 

  • Agüero MA, Aron L, DeLuca AG, Timmis KN, Cabello FC (1984) A plasmid-encoded outer membrane protein, traT, enhances resistance of Escherichia coli to phagocytosis. Infect Immun 46:740–746

    PubMed  Google Scholar 

  • Bayer ME (1979) The fusion sites between outer membrane and inner membrane of bacteria: their role in membrane assembly and virus infection. In: Inouye M (ed) Bacterial outer membranes. Wiley, New York

    Google Scholar 

  • Bhattachaijee AK, Jennings HJ, Kenny CP, Martin A, Smith ICP (1975) Structural determination of the sialic polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. J Biol Chem 250:1926–1932

    Google Scholar 

  • Binns MM, Davies DL, Hardy KG (1979) Cloned fragments of the plasmid ColV, I-K94 specifying virulence and serum resistance. Nature 279:778–781

    Article  PubMed  CAS  Google Scholar 

  • Binns MM, Mayden J, Levine RP (1982) Further characterization of complement resistance conferred on Escherichia coli by the plasmid genes TraT of R100 and iss of ColV, I-K94. Infect Immun 35:654–659

    PubMed  CAS  Google Scholar 

  • Bitter-Suermann D, Peters H, Jurs M, Nehrbass R, Montenegro M, Timmis KN (1984) Monoclonal antibody detection of IncF group plasmid-encoded traT protein in clinical isolates of Escherichia coli. Infect Immun 46:308–313

    PubMed  CAS  Google Scholar 

  • Buchanan TM, Pierce WA (1979) Pathogenic aspects of outer membrane components of Gram negative bacteria. In: Inouye M (ed) Bacterial outer membranes. Wiley, New York, pp 475–514

    Google Scholar 

  • Cabello F, Timmis KN (1979) Plasmids of medical importance. In: Timmis KN, Puhler A (eds) Plasmids of medical, environmental and commercial importance. Elsevier, Amsterdam, pp 55–69

    Google Scholar 

  • Clancy J, Savage DC (1981) Another colicin V phenotype: in vitro adhesion of Escherichia coli to mouse intestinal epithelium. Infect Immun 32:343–352

    PubMed  CAS  Google Scholar 

  • Comb DG, Roseman S (1960) The sialic acids. I. The structure and enzymic synthesis of iV-acetylneuraminic acid. J Biol Chem 235:2529–2537

    PubMed  CAS  Google Scholar 

  • Comb DG, Shimizu F, Roseman S (1959) Isolation of cytidine-S’-monophosphate-iV-acetylneuraminic acid. J Am Chem Soc 81:5513–5514

    Article  CAS  Google Scholar 

  • Costerton JW (1977) Cell envelope as a barrier to antibiotics. In: Schlessinger D (ed) Microbiology-1977. American Society for Microbiology, Washington DC, pp 151–157

    Google Scholar 

  • Cross AS, Zollinger W, Mandreil R, Gemski P, Sadoff J (1983) Evaluation of immunotherapeutic approaches for the potential treatment of infections caused by Kl-positive Escherichia coli. J Infect Dis 147:68–76

    Article  PubMed  CAS  Google Scholar 

  • Cross AS, Gemski P, Sadoff JC, Ørskov F, Ørskov I (1984) The importance of the K1 capsule in invasive infections caused by Escherichia coli. J Infect Dis 149:184–193

    Article  PubMed  CAS  Google Scholar 

  • Echarti CE, Hirschel B, Boulnois GJ, Yarley JM, Waldvogel F, Timmis KN (1983) Cloning and analysis of the Kl capsule biosynthesis genes of Escherichia coli: lack of homology with Neisseria meningitidis group B DNA sequences. Infect Immun 41:54–60

    PubMed  CAS  Google Scholar 

  • Elwell LP, Shipley PL (1980) Plasmid-mediated factors associated with virulence of bacteria to animals. Annu Rev Microbiol 34:465–496

    Article  PubMed  CAS  Google Scholar 

  • Falkone G, Campa M, Smith H, Scott GM (1984) Bacterial and viral inhibition and modulation of host defenses. Academic, London

    Google Scholar 

  • Fietta A, Romero E, Siccardi AG (1977) Effect of some R factors on the sensitivity of rough Enterobacteriaceae to human serum. Infect Immun 18:278–282

    PubMed  CAS  Google Scholar 

  • Frosch M, Görgen I, Boulnois GJ, Timmis KN, Bitter-Suermann D (1985) NZB mouse system for production of monoclonal antibodies to weak bacterial antigens: isolation of an IgG antibody to the polysaccharide capsules of Escherichia coli K1 and group B meningococci. Proc Natl Acad Sci USA 82:1194–1198

    Article  PubMed  CAS  Google Scholar 

  • Gemski P, Cross AS, Sadoff JC (1980) Kl antigen-associated resistance to the bactericidal activity of serum. FEMS Microbiol Lett 9:193–197

    Article  Google Scholar 

  • Ghosh S, Roseman S (1965) The sialic acids. IV. N-acyl -d-glucosamine-6-phosphate epimerase. J Biol Chem 240:1525–1530

    PubMed  CAS  Google Scholar 

  • Glode MP, Lewin EB, Sutton A, Le CT, Gotschlich EC, Robbins JB (1979) Comparative immunogenicity of vaccines prepared from capsular polysaccharides of group C Neisseria meningitidis O- acetyl-positive and O-acetyl-negative variants and Escherichia coli K92 in adult volunteers. J Infect Dis 139:52–59

    Article  PubMed  CAS  Google Scholar 

  • Goldman RC, Joiner K, Leive L (1984) Serum-resistant mutants of Escherichia coli 0111 contain increased lipopolysaccharide, lack an O antigen-containing capsule, and cover more of their lipid A core with O antigen. J Bacteriol 159:877–882

    PubMed  CAS  Google Scholar 

  • Gottschlich EC, Frazer BA, Nishimura O, Robbins JB, Liu T-Y (1981) Lipid on capsular polysaccharides of Gram negative bacteria. J Biol Chem 256:8915–8921

    Google Scholar 

  • Grunstein M, Hogness DS (1975) Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3965

    Article  PubMed  CAS  Google Scholar 

  • Harris JR, Wachsmuth IK, Davis BR, Cohen ML (1982) High-molecular-weight plasmid correlates with Escherichia coli invasiveness. Infect Immun 37:1295–1298

    PubMed  CAS  Google Scholar 

  • Ichihara N, Ishimoto N, Ito E (1974) Enzymic incorporation of iV-acetylmannosaminuronic acid and d-glucose in a polysaccharide of Escherichia coli 014:K7:H —., FEBS Lett 40:309–311

    Article  PubMed  CAS  Google Scholar 

  • Jennings HJ (1983) Capsular polysaccharides as human vaccines. Adv Carbohydr Chem Biochem 41:155–208

    Article  PubMed  CAS  Google Scholar 

  • Jurs M, Peters H, Timmis KN, Bitter-Suermann D (1984) Immunoblotting with monoclonal antibodies - a highly specific system for detection and identification of bacterial outer membrane proteins. In: Habermehl (ed) Rapid methods and automation in microbiology and immunology. Springer Berlin Heidelberg New York Tokyo

    Google Scholar 

  • Kaijser B, Hanson LA, Jodal V, Linden-Johnson G, Robbins JB (1977) Frequency of Escherichia coli K antigens in urinary tract infection in children. Lancet 1:664–666

    Google Scholar 

  • Kopecko DJ, Washington O, Formal SB (1980) Genetic and physical evidence for plasmid control of Shigella sonnei form I cell surface antigen. Infect Immun 29:207–214

    PubMed  CAS  Google Scholar 

  • Kundig FD, Aminoff D, Roseman S (1971) The sialic acids XII. Synthesis of colominic acid by sialyltransferase from Escherichia coli. J Biol Chem 246:2543–2550

    PubMed  CAS  Google Scholar 

  • Levy SB, Clowes RC, Koenig EL (1981) Molecular biology, pathogenicity and ecology of bacterial plasmids. Plenum, New York

    Google Scholar 

  • Liu T-Y, Gottschlich EC, Dunne FT, Jonsen EK (1971) Studies on the meningococcal polysaccharides. Composition and chemical properties of the group B and group C polysaccharides. J Biol Chem 254:4703–4721

    Google Scholar 

  • MacLeod CM, Krauss MR (1950) Relation of virulence of pneumococcal strains to the quantity of capsular polysaccharide formed in vitro. J Exp Med 92:1–9

    Article  PubMed  CAS  Google Scholar 

  • Mäkelä PH, Bradley DJ, Brandis H, Frank MM, Hahn H, Henkel W, Jann K, Morse SA, Robbins RB, Rosenstreich DL, Smith H, Timmis K, Tomasz A, Turner MJ, Wiley DS (1980) Evasion of host defenses. In: Smith H, Skehel JJ, Turner MJ (eds) The molecular basis of microbial pathogenicity. Dahlem Konferenzen. Verlag Chemie, Weinheim, pp 175–198

    Google Scholar 

  • Manning PA, Timmis JK, Moll A, Timmis KN (1982) Mutants that overproduce TraTp, a plasmid- specified major outer membrane protein of Escherichia coli. Mol Gen Genet 187:426—431

    Article  PubMed  CAS  Google Scholar 

  • McCracken GH, Sarff LD, Glode MP, Mize SG, Schiffer MS, Robbins JB, Gotschlich EC, Orskov I, Orskov F (1974) Relation between Escherichia coli K1 antigen polysaccharide antigen and clinical outcome in neonatal meningitis. Lancet II: 246–250

    Article  Google Scholar 

  • McGuire EJ, Binkley SB (1964) The structure and chemistry of colominie acid. Biochemistry 3:247–251

    Article  PubMed  CAS  Google Scholar 

  • Mims CA (1982) The pathogenesis of infectious disease, 2nd edn. Academic, London

    Google Scholar 

  • Minkley EG Jr (1984) Purification and characterization of pro-TraTp, the signal sequence-containing precursor of a secreted protein encoded by the F sex factor. J Bacteriol 158:464–473

    PubMed  CAS  Google Scholar 

  • Minshew BH, Jorgensen J, Swanstrum M, Grootes-Reuvecamp GA, Falkow S (1978) Some characteristics of Escherichia coli strains isolated from extraintestinal infections of humans. J Infect Dis 137:648–654

    Article  PubMed  CAS  Google Scholar 

  • Moll A, Cabello FC, Timmis KN (1979) Rapid assay for the determination of bacterial resistance to the lethal activity of serum. FEMS Lett 6:273–276

    Article  CAS  Google Scholar 

  • Moll A, Manning PA, Timmis KN (1980) Plasmid-determined resistance to serum bactericidal activity: a major outer membrane protein, the traT gene product, is responsible for plasmid-specified serum resistance in Escherichia coli. Infect Immun 28:359–367

    PubMed  CAS  Google Scholar 

  • Montenegro MA, Bitter-Suermann D, Timmis JK, Agüero ME, Cabello FC, Timmis KN (1985) traT gene sequences, serum resistance and pathogenicity-related factors in clinical isolates of Escherichia coli and other Gram negative bacteria. J Gen Microbiol 131, in press

    Google Scholar 

  • Ørskov I, Nyman K (1974) Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli. J Bacteriol 120:43–51

    PubMed  Google Scholar 

  • Ørskov I, Ørskov F, Jann B, Jann K (1977) Serology, chemistry and genetics of O and K antigens of Escherichia coli. Bacteriol Rev 41:667–719

    PubMed  Google Scholar 

  • Ørskov F, Ørskov I, Sutton A, Schneerson R, Lin W, Egan W, Hoff GE, Robbins JB (1979) Form variation of Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide. J Exp Med 149:669–685

    Article  PubMed  Google Scholar 

  • Ørskov I, Sharma V, Orskov F (1976) Genetic mapping of the Kl and K4 antigens (L) of Escherichia coli. Non allelism of K(L) antigens with K antigens of 08:K27(A), 08:K8(L) and 09:K57(B). Acta Pathol Microbiol Scand 84:125–131

    Google Scholar 

  • Ozanne G, Mathieu LG, Baril JP (1977) Production de colicines V et V2 et in vivo etude de leur action inhibitrice sur la phagocytose par des macrophages peritoneaux. Rev Can Biol 36:307–316

    PubMed  CAS  Google Scholar 

  • Paakkanen J, Gotschlich EC, Makela PH (1979) Protein K: a new major outer membrane protein found in encapsulated Escherichia coli. J Bacteriol 139:835–841

    PubMed  CAS  Google Scholar 

  • Penn CW (1983) Bacterial envelope and humoral defenses. In: Easmon CSF, Jeljaszewicz J, Brown MRW, Lambert PA (eds) Medical microbiology vol 3. Role of the envelope in the survival of bacteria in infection. Academic, London, pp 109–135

    Google Scholar 

  • Perumal NB, Minkley EG Jr (1984) The product of the F sex factor traT surface exclusion gene is a lipoprotein. J Biol Chem 259:5357–5360

    PubMed  CAS  Google Scholar 

  • Pittmann M (1931) Variation and type specificity in the bacterial species Haemophilus influenzae. J Exp Med 53:471–492

    Article  Google Scholar 

  • Pluschke G, Mayden J, Achtman M, Levine RP (1983) Role of the capsule and the O antigen in resistance of 018:K1 Escherichia coli to complement-mediated killing. Infect Immun 42:907–913

    PubMed  CAS  Google Scholar 

  • Reynard AM, Beck ME (1976) Plasmid-mediated resistance to the bactericidal effects of normal rabbit serum. Infect Immun 14:848–850

    PubMed  CAS  Google Scholar 

  • Robbins JB, Myerowitz RL, Whisnant JK, Argaman M, Schneerson R, Handzel ZT, Gottschlich EC (1972) Enteric bacteria cross reactive with Neisseria meningitidis groups A and C and Diplococcus pneumoniae types I and III. Infect Immun 6:651–656

    PubMed  CAS  Google Scholar 

  • Robbins JB, Schneerson R, Egan WB, Vann W, Lui DT (1980) Virulence properties of bacterial capsular polysaccharides - unanswered questions. In: Smith H, Skehel JJ, Turner MJ (eds) The molecular basis of microbial pathogenicity. Dahlem Konferenzen. Verlag Chemie, Weinheim, pp 115–132

    Google Scholar 

  • Rohr TE, Troy FA (1980) Structure and biosynthesis of surface polymers containing polysialic acid in Escherichia coli. J Biol Chem 255:2332–2342

    PubMed  CAS  Google Scholar 

  • Sansonetti PJ, Kopecko DJ, Formal SB (1982) Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun 35:853–860

    Google Scholar 

  • Sarff LD, McCracken GH Jr, Schiffer MS, Glode MP, Robbins JB, Ørskov I, Ørskov F (1975) Epidemiology of Escherichia coli K1 in healthy and diseased newborns. Lancet 1:1090–1104

    Google Scholar 

  • Schauer R (1982) Chemistry, metabolism, and biological functions of sialic acids. Adv Carbohydr Chem Biochem 40:131–234

    Article  PubMed  CAS  Google Scholar 

  • Schmidt MA, Jann K (1982) Phospholipid substitution of capsular (K) polysaccharide antigens from Escherichia coli causing extra intestinal infections. FEMS Microbiol Letts 14:69–74

    Article  CAS  Google Scholar 

  • Silver RP, Finn CW, Vann WF, Aaronson W, Schneerson R, Kretschmer P, Garon CF (1981) Molecular cloning of the K1 capsular polysaccharide biosynthesis genes of E. coli. Nature 289:696–698

    Article  PubMed  CAS  Google Scholar 

  • Silver RP, Vann WF, Aaronson W (1984) Genetic and molecular analysis of Escherichia coli K1 antigen genes. J Bacteriol 157:568–575

    PubMed  CAS  Google Scholar 

  • Söderström T, Stein K, Brinton CC Jr, Hosea S, Buch C, Hansson HA, Karpas A, Schneerson R, Sutton A, Vann WI, Hanson LA (1983) Serological and functional properties of monoclonal antibodies to Escherichia coli type I pilus and capsular antigens. Prog Allergy 33:259–274

    PubMed  Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 127:502–517

    Google Scholar 

  • Sturm S, Fortnagel P, Timmis KN (1985) Immunoblotting procedure for the analysis of electrophoretically-fractionated bacterial lipopolysaccharide. Arch Microbiol 140:198–201

    Article  Google Scholar 

  • Sutcliffe J, Blumenthal R, Walter A (1983) Escherichia coli K protein is a porin. J Bacteriol 156:867–872

    PubMed  CAS  Google Scholar 

  • Taylor PW (1983) Bactericidal and bacteriolytic activity of serum against Gram negative bacteria. Microbiol Rev 47:46–83

    PubMed  CAS  Google Scholar 

  • Taylor PW, Hughes C (1978) Plasmid carriage and the serum sensitivity of enterobacteria. Infect Immun 22:10–17

    PubMed  CAS  Google Scholar 

  • Timmis KN, Moll A, Danbara H (1979) Plasmid gene that specifies resistance to the bactericidal activity of serum. In: Timmis KN, Puhler A (eds) Plasmids of medical, environmental and commercial importance. Elsevier, Amsterdam, pp 145–153

    Google Scholar 

  • Timmis KN, Manning PA, Echarti C, Timmis JK, Moll A (1981) Serum resistance in E. coli. In: Levy SB, Clowes RC, Koenig EL (eds) Molecular biology, pathogenicity and ecology of bacterial plasmids. Plenum, New York, pp 133–144

    Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polysaccharide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    Article  PubMed  CAS  Google Scholar 

  • Troy FA, McCloskey MA (1979) Role of a membranous sialyltransferase complex in the synthesis of surface polymers containing polysialic acid in Escherichia coli. J Biol Chem 254:7377–7487

    PubMed  CAS  Google Scholar 

  • Troy FA, Vijay IK, Tesche N (1975) Role of undecaprenyl phosphate in synthesis of polymers containing sialic acid in Escherichia coli. J Biol Chem 250:156–163

    PubMed  CAS  Google Scholar 

  • Turk DC, May JR (1967) Haemophilus influenzae: its clinical importance. English University Press, London, pp 27–38, 58–70

    Google Scholar 

  • Vann WF, Schmidt A, Jann B, Jann K (1981) The structure of the capsular polysaccharide (K5 antigen) or urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to Desulfo- Heparin. Eur J Biochem 116:359–364

    Article  PubMed  CAS  Google Scholar 

  • Watanabe H, Timmis KN (1984) A small plasmid in Shigella dysenteriae 1 specifies one or more functions essential for O antigen production and bacterial virulence. Infect Immun 43:391–396

    PubMed  CAS  Google Scholar 

  • Watanabe H, Nakamura A, Timmis KN (1984) Small virulence plasmid of Shigella dysenteriae 1 strain W30864 encodes a 41000-dalton protein involved in formation of specific lipopolysaccharide side chains of serotype 1 isolates. Infect Immun 46:55–63

    PubMed  CAS  Google Scholar 

  • Whitfield C, Hancock REW, Costerton JW (1983) Outer membrane protein K of Escherichia coli: purification and pore forming properties in lipid membranes. J Bacteriol 156:873–879

    PubMed  CAS  Google Scholar 

  • Williams PH (1979) Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun 26:925–932

    PubMed  CAS  Google Scholar 

  • Williams Smith H (1974) A search for transmissible pathogenic characters in invasive strains of Escherichia coli: the discovery of a plasmid-controlled toxin and a plasmid-controlled lethal character closely associated, or identical, with colicine V. J Gen Microbiol 83:95–111

    PubMed  CAS  Google Scholar 

  • Wolfberg G, DeWitt CW (1969) Mouse virulence of K(L) antigen-containing strains of Escherichia coli. J Bacteriol 100:730–737

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Medical Biochemistry, University of Geneva, CH-1211, Geneva, Switzerland

    K. N. Timmis & G. J. Boulnois

  2. Department of Microbiology, University of Leicester, Leicester, Great Britain

    G. J. Boulnois

  3. Institute of Medical Microbiology, University of Mainz, D-6500, Mainz, Germany

    D. Bitter-Suermann

  4. Department of Microbiology, New York Medical College, Valhalla, New York, USA

    F. C. Cabello

Authors
  1. K. N. Timmis
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  2. G. J. Boulnois
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  3. D. Bitter-Suermann
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  4. F. C. Cabello
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Editors and Affiliations

  1. Institut für genetik und Mikrobiologie, Universität Würzburg, Röntgenring 11, D-8700, Würzburg, Germany

    W. Goebel

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Timmis, K.N., Boulnois, G.J., Bitter-Suermann, D., Cabello, F.C. (1985). Surface Components of Escherichia coli That Mediate Resistance to the Bactericidal Activities of Serum and Phagocytes. In: Goebel, W. (eds) Genetic Approaches to Microbial Pathogenicity. Current Topics in Microbiology and Immunology, vol 118. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70586-1_11

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