Genetic Analysis of Bacterial Virulence Determinants in Bordetella pertussis and the Pathogenic Yersinia
With the advent of recombinant DNA technology, rapid advances have been made in understanding the molecular organization of bacterial pathogens (for review, see Macrina 1984). This has been especially true in the analysis of enterotoxin structure and function (So et al. 1976, 1978; MOSELEYand FALKOW1980; PEARSONand MEKALANOS1982) as well as in the evaluation of bacterial pili and hemolysins in gastrointestinal or extraintestinal infections (SVANBORG-EDEN and HANSSON 1978; WELCH etal. 1981; HARTLEINetal. 1983). Similarly, such studies have clarified the role of iron uptake in the infectious process (CROSA1984) and have been helpful in understanding better the molecular epidemiology of disease agents (HULLet al. 1984). We suppose that in the near future most virulence factors defined by classic studies in medical microbiology will be analyzed at the genetic level in some manner or other.
KeywordsManifold Recombination Tuberculosis Hull Stein
Unable to display preview. Download preview PDF.
- Kasuga T, Nakase Y, Ukishima K, Takatsu K (1953) Studies on Haemophilus pertussis. Kitasato Arch Exp Med 27:37–48Google Scholar
- Lindberg FP, Lund B, Normark S (1984) Genes of pyelonephritogenic Escherichia coli required for digalactoside-specific agglutination of human cells. EMBOJ 3:1167–1173Google Scholar
- Merchant T, Parker A (1962) Veterinary Bacteriology and Virology. Bailliere, Tindall and Cox, London, pp 431–435Google Scholar
- Moseley SL, Falkow S (1980) Nucleotide sequence homology between the heat labile enterotoxin gene of Escherichia coli and Vibrio cholera DNA. J Bacteriol 144:441–446Google Scholar
- Svanborg-Eden C, Hansson HA (1978) Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infect Immun 21:229–237Google Scholar
- Weiss AA, Falkow S (1983) The use of molecular techniques to study microbial determinants of pathogenicity. Phil Trans R Soc Lond B303:219–225Google Scholar
- Weiss AA, Stibitz S, Falkow S (1985) Cloning and expression of the Bordetella pertussis vir gene in Escherichia coli.Google Scholar