Abstract
Plant germplasm preservation is becoming increasingly urgent. Freeze storage in liquid nitrogen is a suitable method for plant and animal lines. Liquid nitrogen is readily available commercially and relatively cheap to buy in many countries. It has been well known that plant materials such as seeds, pollen, tubers, etc., which resist an intensive dehydration, retain their viability after direct immersion in liquid nitrogen from room temperature when rewarmed slowly or rapidly (Sakai and Noshiro 1975).
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© 1986 Springer-Verlag Berlin Heidelberg
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Sakai, A. (1986). Cryopreservation of Germplasm of Woody Plants. In: Bajaj, Y.P.S. (eds) Trees I. Biotechnology in Agriculture and Forestry, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70576-2_8
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DOI: https://doi.org/10.1007/978-3-642-70576-2_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-70578-6
Online ISBN: 978-3-642-70576-2
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