Abstract
Chemically synthesized DNA fragments are versatile tools in modern bioscience. They are nowadays routinely used not only in recombinant DNA projects as probes, primers, linkers, and adaptors, but also in the construction of complete genes. The present technology for total gene synthesis is described. We believe that further improvements in the methodology will make the present instrumentation for DNA synthesis obsolete, enable us to circumvent, when desired, techniques such as site-directed mutagenesis and open up better possibilities, e.g., in the design of proteins. In addition, we consider social responsibilities of DNA chemists.
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© 1986 Dr. S. Bernhard, Dahlem Konferenzen, Berlin
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Blöcker, H., Frank, R. (1986). Synthetic Genes. In: Silver, S. (eds) Biotechnology: Potentials and Limitations. Dahlem Workshop Reports, vol 35. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70535-9_4
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DOI: https://doi.org/10.1007/978-3-642-70535-9_4
Publisher Name: Springer, Berlin, Heidelberg
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