Hydrogen Ion Variations and Their Interpretation in the Microenvironment of the Vertebrate Brain

  • C. Nicholson
  • R. P. Kraig
  • C. R. Ferreira-Filho
  • P. Thompson


The introduction [1] of a reliable liquid membrane ion-selective micropipette (ISM) for H+ has opened up an important new window onto ion and metabolic homeostasis in brain tissue. Formerly, glass membrane ISMs were commonly used [14], but frequently the response was too slow to capture H+ transients. They were also difficult to fabricate as double-barrelled electrodes, i. e., with an integral reference barrel [2]. An alternative type of ISM for H+ employed antimony as the sensor and has been variously configured (e.g., [5]). This sensor, however, is subject to a variety of ill-defined interferences [10]. The liquid membrane ISM overcomes virtually all the deficiencies of previous sensors (note, however, that dinitrophenol and presumably other lipophilic proton transporting anions, destabilize the ISM). We have made an extensive study of pH changes in the cerebellar microenvironment of the in vivo rat [4]. More recently, we have studied the microenvironment of the in vitro turtle cerebellum. We shall draw on both these studies here.


Liquid Membrane Spreading Depression Neutral Carrier Brain Microenvironment Previous Sensor 
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© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • C. Nicholson
  • R. P. Kraig
  • C. R. Ferreira-Filho
  • P. Thompson

There are no affiliations available

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