Brain Extracellular pH Changes During Alterations in Substrate Supply

  • W. A. C. Mutch
  • A. J. Hansen


The alterations in brain extracellular ionic composition during spreading depression (SD), anoxia, ischemia, and profound hypoglycemia have been clarified to a large extent through the use of double-barrelled liquid ion exchanger (LIX) microelectrodes. Recently, a neutral carrier H+- LIX has become available, permitting examination of the extracellular pH (pHe) changes associated with these events [1, 9]. Construction of pH microelectrodes with this exchanger provide the specific advantages of double-barreled LIX microelectrodes, i. e., small tip diameters (2–4 pm as used in these studies), an immediately adjacent reference electrode, and rapid transient response (90% response in ≤ 2 s). We examined the pHe changes during SD and also the pHe changes during events which are initiated by altered substrate supplies, i. e., cerebral ischemia, terminal anoxia, and profound hypoglycemia (the hypoglycemia experiments are work in progress by W. A. C. Mutch in association with A. R. Gardner-Medwin).


Cerebral Ischemia Cortical Spreading Depression Spreading Depression Substrate Supply Neutral Carrier 
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  1. 1.
    Ammann D, Lanter F, Steiner RA, Schulthess P, Shijo Y, Simon W (1981) Neutral carrier based hydrogen ion-selective microelectrode for extra- and intracellular studies. Anal Chem 53: 2267–2269PubMedCrossRefGoogle Scholar
  2. 2.
    Astrup J, Norberg K (1976) Potassium activity in cerebral cortex in rats during progressive severe hypoglycemia. Brain Res 103: 418–423PubMedCrossRefGoogle Scholar
  3. 3.
    Astrup J, Heuser D, Lassen NA, Nilsson B, Norberg K, Siesjö BK (1978) Evidence against H+ and K+ as main factors for the control of cerebral blood flow: a microelectrode study. In: Cerebral vascular smooth muscle and its control. Ciba Found Symp 56Google Scholar
  4. 4.
    Bureš J, Burešovä O, Kőivänek J (1974) The mechanisms and applications of Leao’s spreading depression of electroencephalographic activity. Academic, LondonGoogle Scholar
  5. 5.
    Hansen AJ (1977) Extracellular potassium concentration in juvenile and adult rat brain cortex during anoxia. Acta Physiol Scand 99: 412–423PubMedCrossRefGoogle Scholar
  6. 6.
    Hansen AJ, Olsen CE (1980) Brain extracellular space during spreading depression and ischemia. Acta Physiol Scand 108: 355–365PubMedCrossRefGoogle Scholar
  7. 7.
    Hansen AJ, Zeuthen T (1981) Extracellular ion concentrations during spreading depression and ischemia in the rat brain cortex. Acta Physiol Scand 113: 437–445PubMedCrossRefGoogle Scholar
  8. 8.
    Kraig RP, Nicholson C (1978) Extracellular ionic variations during spreading depression. Neu- roscience 3:1045–1059Google Scholar
  9. 9.
    Kraig RP, Ferreira-Filho CR, Nicholson C (1983) Alkaline and acid transients in cerebellar mic- roenvironment. J Neurophysiol 49: 831–850PubMedGoogle Scholar
  10. 10.
    Kőivänek J (1962) Concerning the dynamics of the metabolic changes accompanying cortical spreading depression. Physiol Bohemoslov 11: 383–391Google Scholar
  11. 11.
    Mutch WAC, Hansen AJ (1984) Extracellular pH changes during spreading depression and cerebral ischemia: Mechanisms of brain pH regulation. J Cereb Blood Flow Metabol: 17–27Google Scholar
  12. 12.
    Siesjö BK (1972) The regulation of cerebrospinal fluid pH. Kidney Int 1: 360–374PubMedCrossRefGoogle Scholar
  13. 13.
    Siesjö BK (1978) Brain energy metabolism. Wiley, ChichesterGoogle Scholar
  14. 14.
    Tschirgi RD, Inanaga K, Taylor JL, Walker M, Sonnenschein RR (1957) Changes in cortical pH and blood flow accompanying spreading cortical depression and convulsion. Am J Physiol 190: 557–562PubMedGoogle Scholar
  15. 15.
    Tsacopoulos M, Lehmenkühler A (1977) A double-barrelled Pt-microelectrode for simultaneous measurement of pO2 and bioelectrical activity in excitable tissues. Experientia 33: 1337–1338PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • W. A. C. Mutch
  • A. J. Hansen

There are no affiliations available

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