Skip to main content
SpringerLink
Log in
Book cover

Ion Measurements in Physiology and Medicine pp 122–127Cite as

The Applicability of Fluorescence Indicators to Measure Hydrogen Ion Activities by Optimizing Accuracy and Minimizing the Influence of Ionic Strength

  • Chapter

Abstract

pH-sensitive fluorescence indicators can be used for the measurement of hydrogen ion activities [8,10]. To improve the applicability of fluorescence indicators generally, we have developed special fluorescence sensors, optodes, in which the indicator molecules are separated from the medium by a protecting membrane which is permeable for the substance to be measured [2, 3, 5]. Thus, interactions of proteins or colloids of biological fluids with the indicator molecules can be avoided. To eliminate optical interference due to reflection changes, scattering, and non-specific fluorescence, the sensor membrane has to be prepared for optical insulation.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chen RF (1968) Fluorescent pH indicators: Spectral changes of 4-methylumbelliferone. Anal Lett 1 (7): 423–428

    Article  CAS  Google Scholar 

  2. Lubbers DW, Opitz N (1975) Die pCO2-/pO2-;Optode: Eine neue pCO2- bzw. p2-Meßsonde zur Messung des pCO2oder pO2 von Gasen und Flüssigkeiten, Z Naturforsch 30c: 532–533

    Google Scholar 

  3. Lübbers DW, Opitz N, Speiser PP, Bisson HJ (1977) Nanoencapsulated fluorescence indicator molecules measuring pH and p02 down to submicroscopical regions on the basis of the optode principle. Z Naturforsch 32c: 133–134

    Google Scholar 

  4. Nakashima M, Sousa JA, Clapp RC (1972) Spectroscopic species of 4-methylumbelliferone in water and ethanol. Nature Phys Sci 235: 16

    Google Scholar 

  5. Opitz N (1976) Messung biologischer Gase und Flüssigkeiten mit Hilfe der quantitativen Fluoreszenzphotometrie mit Indikatoren. Thesis, University of Marburg

    Google Scholar 

  6. Opitz N, Lübbers DW (1983) Hochauflösende pO2Analyse mit abstimmbaren Fluoreszenzmeßsonden. Biomed Tech (Berlin) 28: 31

    Google Scholar 

  7. Opitz N, Lübbers DW(1984) New fluorescence photometrical techniques for simultaneous and continuous measurements of ionic strength and hydrogen ion activities. Sensors and Actuators (in press)

    Google Scholar 

  8. Peterson JI, Goldstein SR, Fitzgerald RV (1980) Fiber optic pH probe for physiological use. Anal Chern 52: 864–868

    Article  CAS  Google Scholar 

  9. Rauen HM (1964) Biochemisches Taschenbuch, Part 2. Springer, Berlin Göttingen Heidelberg New York

    Google Scholar 

  10. Tageeva SV, Kosheleva GN, Dubrov AP (1971) Determination of hydrogen ion concentration in some biological objects with fluorescent indicators. Tsitologiya 13 (1): 122–125

    CAS  Google Scholar 

  11. Wolfbeis OS, Fürlinger E, Kroneis H, Marsoner H (1983) Fluorimetric analysis. 1. A study on fluorescent indicators for measuring near neutral-(“physiological”) pH-values. Fresenius Z Anal Chern 314: 577–581

    Article  CAS  Google Scholar 

Download references

Authors
  1. N. Opitz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. W. Lübbers
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut för Physiologie und Kardiologie, Waldstr. 6, D-8520, Erlangen, Germany

    Manfred Kessler , David Keith Harrison  & Jens Höper ,  & 

  2. Dept. of Chemistry, University of North Carolina, Chapel Hill, NC, 27514, USA

    R. P. Buck

  3. Dept. of Physiology, School of Medicine, The Center for Health Science, University of California, Los Angeles, California, 90024, USA

    G. Eisenman

  4. Biosensors Inc., PO Box 640, Chester, New Jersey, 07930, USA

    J. Hill

  5. Faculty of Medicine, American Univ. of Beirut, Beirut, Lebanon

    R. N. Khuri

  6. Direktor des Max-Planck-Instituts für Systemphysiologie, Rheinlanddamm 201, 4600, Dortmund 1, Germany

    D. W. Lübbers

  7. Medical Center, Dept. of Physiology and Biophysics, New York University, 550 First Avenue, New York, NY, 10016, USA

    C. Nicholson

  8. Laboratorium für Organische Chemie, Universitätsstr. 16, 6000, Frankfurt M. 70, Germany

    W. Simon

  9. The Middlesex Hospital, Mortimer St., London W N, 8AA, England

    T. Treasure

  10. Institute of Medical Physiology, Dept. A, University of Copenhagen, DK-2200, Copenhagen N, Denmark

    T. Zeuthen

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Opitz, N., Lübbers, D.W. (1985). The Applicability of Fluorescence Indicators to Measure Hydrogen Ion Activities by Optimizing Accuracy and Minimizing the Influence of Ionic Strength. In: Kessler, M., et al. Ion Measurements in Physiology and Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70518-2_19

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-70518-2_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-15468-6

  • Online ISBN: 978-3-642-70518-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation