Advertisement

Calcium Currents in Mammalian Central Neurones

  • D. A. Brown
  • R. J. Docherty
  • B. H. Gahwiler
  • J. V. Halliwell
Part of the Bayer-Symposium book series (BAYER-SYMP, volume 9)

Abstract

Voltage-activated Ca-currents (ICa) are present in most neurones (Hagiwara and Byerly 1981; Kostyuk 1981). This article concerns the Ca-currents observed in mammalian central neurones. Their properties have been inferred from a variety of Ca-dependent voltage-transients (reviewed by Llinas and Walton 1980); and Ca-currents have been recorded directly in voltage-clamp experiments on spinal motoneurones (Schwindt and Crill 1980a,b), in hippocampal pyramidal cells (Johnston et al. 1980; Brown and Griffith 1983 b; Halliwell 1983) and in olfactory cortex neurons (A. Constanti and M. Galvan, unpublished observations).

Keywords

Hippocampal Neurone Outward Current Calcium Current Hippocampal Pyramidal Cell Dorsal Root Ganglion Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adams PR, Brown DA, Halliwell JV (1981) Cholinergic regulation of M-current in guinea-pig hippocampal neurones. J Physiol Lond 317:29–30.Google Scholar
  2. Aldenhoff JB, Gruol DL, Rivier J, Vale W, Siggins GR (1983) Corticotrophin releasing factor decreases postburst hyperpolarizations and excites hippocampal neurons. Science NY 221:875–877.CrossRefGoogle Scholar
  3. Barrett JF, Barrett JN, Crill WE (1980) Voltage-sensitive outward currents in cat motoneurones. J Physiol Lond 304:251–276.PubMedGoogle Scholar
  4. Bellemann P, Schade A, Towart R (1983) Dihydropyridine receptor in rat brain labelled with 3H nimodipine Proc Natl Acad Sci USA 80:2356–2360.PubMedCrossRefGoogle Scholar
  5. Benardo LS, Masukawa LM, Prince DA (1982) Electrophysiology of isolated hippocampal pyramidal dendrites. J Neurosci 2:1614–1622.PubMedGoogle Scholar
  6. Benardo LS, Prince DA (1981) Acetylcholine induced modulation of hippocampal pyramidal neurons. Brain Res 211:227–234.PubMedCrossRefGoogle Scholar
  7. Bormann J, Sakmann B, Seifert W (1983) Isolation of GABA-activated single-channel Cl- currents in the soma membrane of rat hippocampal neurones. J Physiol, Lond, 341:9-10P.Google Scholar
  8. Brown DA, Adams PR (1980) Muscarinic suppression of a noval voltage-sensitive K-current in voltage-clamped hippocampal cell somata. Nature, Lond, 183:673–676.CrossRefGoogle Scholar
  9. Brown DA, Griffith WH (1983a) Calcium-activated outward current in voltage-clamped hippocampal neurones of the guinea-pig. J Physiol, Lond, 337:287–301.PubMedGoogle Scholar
  10. Brown DA, Griffith WH (1983) Persistent slow inward current in voltage-clamped hippocampal neurones of the guinea-pig. J Physiol, Lond, 337:303–320.PubMedGoogle Scholar
  11. Brown DA, Constanti A, Docherty RJ, Galvan M, Gahwiler B, Halliwell JV (1984) Pharmacology of calcium currents in mammalian central neurons. Proc 9th Int. Pharmacol Congress (in press).Google Scholar
  12. Brown TH, Fricke RA, Perkel DH (1980) Passive electrical constants in three classes of hippocampal neurons. J Neurophysiol 46:812–827.Google Scholar
  13. Carbonne E, Lux HD (1984) A low voltage-activated, fully inactivating Ca-channel in vertebrate sensory neurones. Nature, Lond, 310:501–502.CrossRefGoogle Scholar
  14. Cherubini E, North RA (1984). Actions of γ-aminobutyric acid on neurones of guinea-pig myenteric plexus. Br J Pharmac 82:93–100.Google Scholar
  15. Cole AE, Nicoll RA (1984) The pharmacology of cholinergic excitatory responses in hippocampal pyramidal cells. Brain Res 305:283–290.PubMedCrossRefGoogle Scholar
  16. Colquhoun D, Neher E, Reuter H, Stevens CF (1981) Inward current channels activated by intracellular Ca in cultured cardiac cells. Nature, Lond, 294:752–754.CrossRefGoogle Scholar
  17. Connor JA, Stevens CF (1971) Voltage-clamp studies of a transient outward membrane current in gastropod neural somata. J Physiol Lond 213:21–30.PubMedGoogle Scholar
  18. Constanti A, Brown DA (1981) M-currents in voltage-clamped mammalian sympathetic neurones. Neurosci Lett 24:289–294.PubMedCrossRefGoogle Scholar
  19. Cortes R, Supavilai P, Karobath M, Palacois JM (1983) The effect of lesions in the rat hippocampus suggest the association of calcium channel blocker binding sites with specific neuronal population. Neurosci Lett 42:249–254.PubMedCrossRefGoogle Scholar
  20. Curtis BM, Catterall WA (1983) Solubilization of the calcium antagonist receptor from rat brain. J Biol Chem 258:7280–7283.PubMedGoogle Scholar
  21. Desarmenien M, Feltz P, Occhipinti G, Santangelo F, Schlichter R (1984) Coexistence of GA-BAA and GABAB receptors on A and C primary afferents. Br J Pharmac 81:327–334.Google Scholar
  22. Deschenes M, Roy JP, Steriade M (1982) Thalamic bursting mechanism: an inward slow current revealed by a membrane hyperpolarization. Brain Res 239:289–293.PubMedCrossRefGoogle Scholar
  23. Dodd J, Dingeldine R, Kelly JS (1980) The excitatory action of acetylcholine on hippocampal neurones of the guinea-pig and rat maintained in vitro. Brain Res 207:109–127.CrossRefGoogle Scholar
  24. Dolly J, Halliwell JV, Black JD, Williams RS, Pelchen-Matthews A, Breeze AL, Mehraban F, Othman IB, Black AR (1984) Botulinum neurotoxin and dendrotoxin as probes for studies on transmitter release. J Physiol Paris (in press).Google Scholar
  25. Dunlap K (1981) Two types of γ-aminobutyric acid receptor on embryonic sensory neurones. Br J Pharmac 74:579–585.Google Scholar
  26. Dunlap K, Fischbach GD (1981) Neuro transmitters decrease the calcium conductance activated by depolarization of embryonic chick sensory neurones. J Physiol, Lond 317:519–535.PubMedGoogle Scholar
  27. Eckert R, Lux HD (1976) A voltage-sensitive persistent calcium conductance in neuronal somata of Helix. J Physiol, Lond 254:129–152.PubMedGoogle Scholar
  28. Fox AP, Nowycky MC, Tsien RW (1984) Single Ca channel in dorsal root ganglion cells are shifted between modes of gating by the calcium agonist Bay K 8644. Neurosci Abstr 10:526.Google Scholar
  29. Freedman SB, Dawson G, Villereal ML, Miller RJ (1984) Identification and characterization of voltage-sensitive calcium channels in neuronal clonal lines. J Neurosci 4:1453–1467.PubMedGoogle Scholar
  30. Gahwiler B (1981) Organotypic monolayer cultures of nervous tissue. J Neurosci Meth 4:329–342.CrossRefGoogle Scholar
  31. Gahwiler B, Brown DA (1985) GABAB-receptor activated K+-current in voltage-clamped CA3 pyramidal cells in hippocampal cultures. Proc Natl Acad Sci USA 82:1558–1562.PubMedCrossRefGoogle Scholar
  32. Gahwiler BH, Dreifuss JJ (1982) Multiple actions of acetylcholine on hippocampal pyramidal cells in organotypic explant cultures. Neuroscience 7:1243–1256.PubMedCrossRefGoogle Scholar
  33. Galvan M, Adams PR (1982) Control of calcium-current in rat sympathetic neurons by norepinephrine. Brain Res 244:135–144.PubMedCrossRefGoogle Scholar
  34. Glossman H, Ferry DR, Lubbecke F, Mewes R, Hofmann F (1982) Calcium channels: direct identification with radioligand binding studies. Trends in Pharmacol Sci 3:431–437.CrossRefGoogle Scholar
  35. Gustafsson B, Galvan M, Grafe P, Wigstrom H (1982) A transient outward current in a mammalian central neurone blocked by 4-aminopyridine. Nature, Lond 299:252–254.CrossRefGoogle Scholar
  36. Haas HL, Konnerth A (1983) Histamine and noradrenaline decrease calcium-activated potassium conductance in hippocampal pyramidal cells. Nature, Lond 302:432–434.CrossRefGoogle Scholar
  37. Hablitz JJ, Johnston D (1981) Endogenous nature of spontaneous bursting in hippocampal pyramidal neurons. J Cell Molec Neurobiol 1:325–334.CrossRefGoogle Scholar
  38. Hagiwara S, Byerly L (1981) Calcium channel. Ann Rev Neurosci 4:69–125.PubMedCrossRefGoogle Scholar
  39. Halliwell JV (1983) Caesium-loading reveals two distinct Ca-currents in voltage-clamped guineapig hippocampal neurones in vitro. J Physiol, Lond 341:10-11P.Google Scholar
  40. Halliwell JV, Adams PR (1982) Voltage-clamp analysis of muscarinic excitation in hippocampal neurons. Brain Res 250:71–92.PubMedCrossRefGoogle Scholar
  41. Halliwell JV, Scholfield CN (1984 a) Adenosine-resistant Ca-currents in mammalian central nervous system. J Physiol, Lond 349:52P.Google Scholar
  42. Halliwell JV, Scholfield CN (1984 b) Somatically-recorded Ca-currents in guinea-pig hippocampal and olfactory cortex neurones are resistant to adenosine action. Neurosci Lett 50:13–18.PubMedCrossRefGoogle Scholar
  43. Henon BK, McAfee DA (1983) Modulation of calcium currents by adenosine receptors on mammalian sympathetic neurones. In: Berne RM, Rall TW, Rabio R (eds) Regulatory function of adenosine. Martinus Nijhoff, The Hague, pp 455-466.Google Scholar
  44. Hotson JR, Prince DA (1981) Penicillin-and barium-induced epileptiform bursting in hippocampal neurons: actions on Ca2+ and K+-potentials. Ann Neurol 10:11–17.PubMedCrossRefGoogle Scholar
  45. Johnston D (1981) Passive cable properties of hippocampal CA3 pyramidal neurons. Cell Mol Neurobiol 1:100–110.CrossRefGoogle Scholar
  46. Johnston D, Brown TH (1984) Mechanisms of neuronal burst generation. In: Schwartzkroin PA, Wheal HV (eds) Electrophysiology of epilepsy. Academic Press, London, pp 278–301.Google Scholar
  47. Johnston D, Hablitz JJ, Wilson WA (1980) Voltage-clamp discloses slow inward current in hippocampal burst-firing neurones. Nature, Lond 286:391–393.CrossRefGoogle Scholar
  48. Kandel ER, Spencer WA (1961) Electrophysiology of hippocampal neurons. II. After-potentials and repetitive firing. J Neurophysiol 24:243–259.PubMedGoogle Scholar
  49. Kostyuk PG (1981) Calcium-channels in the neuronal membrane. Biochem Biophys Acta 650:128–150.PubMedGoogle Scholar
  50. Lancaster B, Adams PR (1984) Single electrode voltage clamp of the slow AHP current in rat hippocampal pyramidal cells. Neurosci Abstr (in press).Google Scholar
  51. Lee KS, Tsien RW (1983) Mechanism of calcium channel blockade by verapamil, D 600, diltiazem and nitrendipine in single dialysed heart cells. Nature, Lond 302:790–794.CrossRefGoogle Scholar
  52. Llinas R, Sugimori M (1980) Electrophysiological properties of in vitro Purkinje cell dendrites in mammalian cerebellar slices. J Physiol, Lond 305:197–213.PubMedGoogle Scholar
  53. Llinas R, Walton K (1980) Voltage-dependent calcium conductances in neurons. In: Cotman CW, Poste G, Nicolson GL (eds) The cell surface and neuronal function. Elsevier, North Holland, pp 87-118.Google Scholar
  54. Llinas R, Yarom Y (1981) Electrophysiology of mammalian inferior olivary neurones in vitro. Different types of voltage-dependent ionic conductances. J Physiol, Lond 315:549–567.Google Scholar
  55. Madison DV, Nicoll RA (1982) Noradrenaline blocks accommodation of pyramidal cell discharge in the hippocampus. Nature, Lond 299:636–638.CrossRefGoogle Scholar
  56. Madison DV, Nicoll RA (1984) Control of the repetitive discharge of CA1 pyramidal neurones in vitro. J Physiol Lond 354:318–331.Google Scholar
  57. Miller RJ, Freedman SB (1984) Are dihydropyridine binding sites voltage-sensitive calcium channels? Life Sci 34:1205–1221.PubMedCrossRefGoogle Scholar
  58. Neher E (1971) The fast transient current components during voltage clamp on snail neurons. J Gen Physiol 58:36–53.PubMedCrossRefGoogle Scholar
  59. Nelson MT, French RJ, Krueger BK (1984) Voltage-dependent calcium channels from brain incorporated into planar lipid bilayers. Nature, Lond 308:77–80.CrossRefGoogle Scholar
  60. Newberry NR, Nicoll RA (1984) Direct hyperpolarizing action of baclofen on hippocampal pyramidal cells. Nature, Lond 308:450–452.CrossRefGoogle Scholar
  61. Nowycky M, Fox AP, Tsien RW (1984) Two components of calcium channel currents in chick dorsal root ganglion cells. Biophys J, 45:36 a.Google Scholar
  62. Numann R, Wong RKS (1984) Voltage-clamp study on GABA response desensitziation in single pyramidal cells dissociated from the hippocampus of adult guinea-pigs. Neurosci, Lett 47:289–294.CrossRefGoogle Scholar
  63. Ogata N, Hori N, Katsuda N (1976) The correlation between extracellular potassium concentration and hippocampal epileptic activity in vitro. Brain Res 110:371–375.PubMedCrossRefGoogle Scholar
  64. Owen DG, Segal M, Barker JL (1984) A Ca-dependent Cl--conductance is present in cultured mouse spinal neurones. Nature, Lond 311:567–570.CrossRefGoogle Scholar
  65. Prince DA (1978) Neurophysiology of epilepsy. Ann Rev Neurosci 1:395–415.PubMedCrossRefGoogle Scholar
  66. Prince DA (1983) Mechanisms of epileptogenesis in brain-slice model systems. In: Ward AA Jr, Penny JK, Purpura D (eds) Epilepsy. Raven Press, New York, pp 29–52.Google Scholar
  67. Proctor WR, Dunwiddie TV (1983) Adenosine inhibits calcium spikes in hippocampal pyramidal neurons in vitro. Neurosci. Lett 35:197–201.PubMedCrossRefGoogle Scholar
  68. Purpura DP, Prelevic S, Santini M (1968) Hyperpolarizing increase in membrane conductance in hippocampal neurons. Brain Res 7:310–312.PubMedCrossRefGoogle Scholar
  69. Reuter H (1983) Calcium channel modulation by neurotransmitters, enzymes and drugs. Nature, Lond 301:569–574.CrossRefGoogle Scholar
  70. Schlichter R, Demeneix BA, Desarmenien M, Desaulles E, Loeffler JP, Feltz P (1984) Properties of the GABA receptors located on spinal primary afferent neurones and hypophyseal neuroendocrine cells of the rat. Neurosci Lett 47:257–263.PubMedCrossRefGoogle Scholar
  71. Schwartzkroin PA (1980) Ionic and synaptic determinants of burst generation. In: Lockard JS, Ward Jr AA (eds) Epilepsy: A window to brain mechanisms. Raven press, New York, pp 83–95.Google Scholar
  72. Schwartzkroin PA, Prince DA (1980) Effect of TEA on hippocampal neurons Brain Res 185:169–181.PubMedCrossRefGoogle Scholar
  73. Schwartzkroin PA, Slawsky M (1977) Probable calcium spikes in hippocampal neurones. Brain Res 135:157–161.PubMedCrossRefGoogle Scholar
  74. Schwindt PC, Crill W (1980 a) Role of a persistent inward current in motoneuron bursting during spinal seizures. J Neurophysiol 43:1296–1318.PubMedGoogle Scholar
  75. Schwindt PC, Crill W (1980 b) Properties of a persistent inward current in normal and TEA-injected motoneurons. J Neurophysiol 43:1700–1724.PubMedGoogle Scholar
  76. Segal M, Barker JL (1984) Rat hippocampal neurones in culture: potassium current. J Neurophysiol 51:1409–1433.PubMedGoogle Scholar
  77. Tillotson D (1979) Inactivation of Ca conductance dependent on entry of Ca ions in molluscan neurons. Proc Nat Acad Sci USA, 76:1497–1500.PubMedCrossRefGoogle Scholar
  78. Toll L (1982) High-affinity binding and inhibition of calcium transport in a clonal cell line. J Biol Chem 257:13189–13192.PubMedGoogle Scholar
  79. Traub RD (1982) Simulation of intrinsic bursting in CA3 hippocampal neurons. Neuroscience 7:1233–1242.PubMedCrossRefGoogle Scholar
  80. Turner DA, Schwartzkroin PA (1980) Steady-state electrotonic analysis of intracellularly stained hippocampal neurons. J Neurophysiol 44:184–199.PubMedGoogle Scholar
  81. Wong RKS, Prince DA (1978) Participation of calcium spikes sharing intrinsic burst firing in hippocampal neurons. Brain Res 159:385–390.PubMedCrossRefGoogle Scholar
  82. Wong RKS, Prince DA (1979) Dendritic mechanisms underlying penicillin-induced epileptiform activity. Science, NY 204:1228–1230.CrossRefGoogle Scholar
  83. Wong RKS, Prince DA, Basbaum AI (1979) Intradendritic recordings from hippocampal neurons. Proc Natl Acad Sci 76:986–990.PubMedCrossRefGoogle Scholar
  84. Wong RKS, Traub RD, Miles R (1984) Epileptogenic mechanisms as revealed by studies of the hippocampal slice. In: Schwartzkroin PA, HV Wheal (eds) Electrophysiology of epilepsy. Academic Press, London, pp 254–275.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • D. A. Brown
  • R. J. Docherty
  • B. H. Gahwiler
  • J. V. Halliwell

There are no affiliations available

Personalised recommendations