Skip to main content
SpringerLink
Log in

DNA Aneuploidy in Childhood Acute Lymphoblastic Leukemia as Detected by Flow Cytometry: Relation to Phenotype, Presentation Features and Prognosis

  • Conference paper
Tumor Aneuploidy

  • 31 Accesses

Abstract

Recent improvements in cytogenetic technology have provided the means to identify chromosomal abnormalities in the majority of children with acute lymphoblastic leukemia (ALL) [4, 18]. In addition to numeric aberrations, specific nonrandom translocations have been described [4, 17]. Cytogenetic studies have also emphasized the clinical significance of chromosomal aberrations in addition to previously identified prognostic factors such as white blood count, sex and age [4, 11, 20]. The applicability of cytogenetic techniques, however, is limited by the laborious and time consuming processing of samples and therefore not suitable for routine clinical evaluations.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aiuti F, Cerottini J, Coombs R, Cooper M, Dickler H, Froland S, Fudenberg H, Greaves M, Grey H, Kunkel H, Natvig J, Preud’homme J, Rabellino E, Ritts R, Rowe D, Seligman M, Siegal F, Terry W, Wybran J (1974) Special technical report: Identification, enumeration and isolation of B-and T-lymphocytes from human peripheral blood. Scan J Immunol 3:521–532

    Article  Google Scholar 

  2. Andreeff M, Miller D, Steinherz P, Kempin S, Straus D, Clarkson B (1981) Hypodiploid acute lymphoblastic leukemia (ALL): a rare entity detected and monitored by flow cytometry. Proc AACR 22:44

    Google Scholar 

  3. Barlogie B, Hittelman W, Spitzer G, Hart JS, Trujillo JM, Smallwood L, Drewinko B (1977) Correlation of DNA distribution abnormalities with cytogenetic findings in human adult leukemia and lymphoma. Cancer Res 37:4400–4407

    CAS  PubMed  Google Scholar 

  4. Bloomfield CD, Rowley JD, Goldmann AI, Lawler SD, Secker Walker LM, Mitelman F (1983) Chromosomal abnormalities and their clinical significance in acute lymphoblastic leukemia. Third International Workshop on Chromosomes in Leukemia. Cancer Res 43:868–873

    Google Scholar 

  5. Brodeur GM, Williams DL, Look AT, Bowman WR, Kalwinsky DK (1981) Near-haploid acute lymphoblastic leukemia: A unique subgroup with a poor prognosis? Blood 58:14–19

    CAS  PubMed  Google Scholar 

  6. Cutler S, Ederer F (1958) Maximum utilsation of the 1fe table method in analysing survival. J Chron Dis 4:699–713

    Article  Google Scholar 

  7. Henze G, Langermann HJ, Fengler R, Brandeis M, Evers KG, Gadner H, Hinderfeld L, Jobke A, Kornhuber B, Lampert F, Lasson U, Ludwig R, Müller-Weihrich S, Neidhardt M, Nessler G, Niethammer D, Rister M, Ritter J, Schaaff A, Schellong G, Stollmann B, Treuner J, Wahlen W, Weinel P, Wehinger H, Riehm H (1982) Bericht über die Therapiestudie BFM 79/81 zur Behandlung der akuten lymphoblastischen Leukämie bei Kindern und Jugendlichen: Intensivierte Reinduktionstherapie für Patientengruppen mit unterschiedlichem Rezidivrisiko. Klin Pädiatr 194:195–203

    Article  CAS  PubMed  Google Scholar 

  8. Henze G, Langermann HJ, Riehm H (1982) Acute lymphoblastic leukemia therapy study BFM 81/83: Stratified treatment for patients with different risk for relapse estimated by the leukemic cell burden. 3rd Int. Symposium on Therapy of Acute Leukemias, Rome

    Google Scholar 

  9. Hiddemann W, Wörmann B, Ritter J, Henze G, Langermann HJ, Kaufmann U, Schellong G, Riehm H, Büchner T(1982) Diagnostik von Aneuploidien bei akuten Leukämien mittels Impulscytophotometrie (ICP): Häufigkeit und klinische Relevanz. Verh Dtsch Ges Inn Med 88:934–937

    Google Scholar 

  10. Jondal M, Holm G, Wigzell H (1972) Surface markers on human B-and T-lymphocytes. I. A large population of lymphocytes forming non immune rosettes with sheep red blood cells. J Exp Med 136:207–212

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Kaneko Y, Rowley JD, Variakojis D, Chilcote RR, Check I, Sakurai M (1982) Correlation of karyotype with clinical features in acute lymphoblastic leukemia. Cancer Res 42:2918–2929

    CAS  PubMed  Google Scholar 

  12. Langermann HJ, Henze G, Wulf M, Riehm H (1982) Mathematisches Modell zur Abschätzung der Blastenmasse der akuten lymphoblastischen Leukämie: Prognostische Bedeutung. Klin Pädiatr 194:209–213

    Article  CAS  PubMed  Google Scholar 

  13. Look AT, Melvin SL, Williams DL, Brodeur GM, Dahl GV, Kalwinsky DK, Murphy SB, Mauer AM (1982) Aneuploidy and percentage of S-phase cells determined by flow cytometry correlate with cell phenotype in childhood acute leukemia. Blood 60:959–967

    CAS  PubMed  Google Scholar 

  14. Look AT, Melvin SL, Williams DL, Roberson PK, Bowman WP, Dahl GV, Murphy SB, Mauer AM (1982) Clinical and biological implications of flow cytometry determination of aneuploidy and pretreatment % S-phase of marrow blasts in childhood acute lymphoblastic leukemia (ALL). 6th Int. Symposium on Flow Cytometry, Elmau

    Google Scholar 

  15. Riehm H, Henze G, Langermann HJ, Odenwald E, Müller-Weihrich S (1983) Childhood B-type Non-Hodgkin’s Lymphoma: Improved prognosis in two consecutive BFM studies. 13th Int. Congress of Chemotherapy, Vienna

    Google Scholar 

  16. Ritter J (1982) Akute Leukämien bei Kindern: Phänotyp und DNS-Gehalt der Blasten in Korrelation zum klinischen Erscheinungsbild und Krankheitsverlauf. Habilitationsschrift, Universität Münster

    Google Scholar 

  17. Roth D, Cimino M (1979) B-cell lymphoblastic leukemia with a 14q+ chromosome abnormality. Blood 53:235–243

    CAS  PubMed  Google Scholar 

  18. Rowley J (1978) The cytogenetics of acute leukaemia. Clin Haematol 7:385–406

    Article  CAS  PubMed  Google Scholar 

  19. Weite K, Ebener K, Hinderfeld L, Ritter J, Henze G, Kornhuber B (1981) Die Bedeutung der terminalen Deoxynucleotidyl-Transferase in der Diagnostik der akuten Leukämien des Kindes. Klin Pädiatr 193:165–171

    Article  Google Scholar 

  20. Williams DL, Tsiatis A, Brodeur GM, Look AT, Melvin SL, Bowman WP, Kalwinsky DK, Rivera G, Dahl GV (1982) Prognostic importance of chromosome number in 136 untreated children with acute lymphoblastic leukemia. Blood 60:864–871

    CAS  PubMed  Google Scholar 

  21. Yunis JJ (1983) The chromosomal basis of human neoplasia. Science 221:227–236

    Article  CAS  PubMed  Google Scholar 

  22. Zante J, Schumann J, Barlogie B, Göhde W, Büchner T (1976) New preparing and staining procedures for specific and rapid analysis of DNA distribution. In: Göhde W, Schumann J, Büchner T (eds) Pulse Cytophotometry II, European Press Medikon, Ghent 97–106

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Univ.-Kinderklinik, Robert-Koch-Str. 31, 4400, Münster, Germany

    J. Ritter

Authors
  1. J. Ritter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Hiddemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Wörmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Schellong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Th. Büchner
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Medizinische Klinik und Poliklinik der Universität, Albert-Schweitzer-Str. 33, D-4400, Münster, Germany

    Th. Büchner  & W. Hiddemann  & 

  2. Section of Medical Oncology, University of Minnesota School of Medicine, Coleman Leukemia Research and Treatment Center, Box 277 University of Minnesota Hospitals, Minneapolis, Minnesota, 55455, USA

    Clara D. Bloomfield

  3. Department of Oncology and Hematology, Medizinische Universitätsklinik Hamburg-Eppendorf, Martinistr. 52, D-2000, Hamburg 20, Germany

    D. K. Hossfeld

  4. Fachklinik Hornheide, Dorbaumstr. 300, D-4400, Münster, Germany

    J. Schumann

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Ritter, J., Hiddemann, W., Wörmann, B., Schellong, G., Büchner, T. (1985). DNA Aneuploidy in Childhood Acute Lymphoblastic Leukemia as Detected by Flow Cytometry: Relation to Phenotype, Presentation Features and Prognosis. In: Büchner, T., Bloomfield, C.D., Hiddemann, W., Hossfeld, D.K., Schumann, J. (eds) Tumor Aneuploidy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70458-1_8

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-70458-1_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-15376-4

  • Online ISBN: 978-3-642-70458-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation