Skin Models pp 218-236 | Cite as

The Development of Epidermis In Vitro. Electronmicroscopic and Autoradiographic Studies in the Limb Bud Culture

  • U. Schultz-Ehrenburg
Conference paper


A problem of epidermal culture systems is to obtain organotypical differentiation in vitro. For that purpose, the presence of dermal factors is of great importance. The epithelio-mesenchymal interaction is best presented in organ cultures of skin, but adult skin explants show short survival times and a progressive loss of maturation. The most successful culture condition is the embryonal organ culture because it is able not only to survive and to grow, but also to differentiate and to mature.


Stratum Corneum Basal Cell Layer Stratum Granulosum Apical Ectodermal Ridge Glycoprotein Synthesis 
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  1. 1.
    Adachi K, Iizuka H, Halprin KM, Levine V (1980) Epidermal cyclic AMP is not decreased in psoriasis lesions. J Invest Dermatol 74: 74–76PubMedGoogle Scholar
  2. 2.
    Aydelotte MB, Kochhar DM (1972) Development of mouse limb buds in organ culture: chondrogenesis in the presence of a proline analog, L-azetidine-2-carboxylic acid. Dev Biol 28: 191–201PubMedCrossRefGoogle Scholar
  3. 3.
    Benett G, Leblond CP, Haddad A (1974) Migration of glycoprotein from Golgi apparatus to the surface of various cell types as shown by radioautography after labeled fucose injections into rats. J Cell Biol 60: 258–284CrossRefGoogle Scholar
  4. 4.
    Delescluse C, Colburn NH, Duell EA, Voorhees JJ (1974) Cyclic AMP-elevating agents inhibit proliferation of keratinizing guinea pig epidermal cells. Differentiation 2: 343–350PubMedCrossRefGoogle Scholar
  5. 5.
    Delescluse C, Fukuyama K, Epstein W (1976) Dibutyryl cyclic AMP-induced differentiation of epidermal cells in tissue culture. J Invest Dermatol 66: 8–13CrossRefGoogle Scholar
  6. 6.
    Elias PM (1981) Membranes, lipids, and the epidermal permeability barrier. In: Marks R, Christophers E (eds) The epidermis in disease. MTP Press, Lancaster, pp 1–30Google Scholar
  7. 7.
    Friedman DL (1976) Role of cyclic nucleotides in cell growth and differentitation. Physiol Reviews 56: 652–708Google Scholar
  8. 8.
    Fukuyama K, Epstein WL (1971) Inhibition of RNA and protein synthesis in granular cells by actinomycin-D and puromycin. J Invest Dermatol 56: 211–222PubMedCrossRefGoogle Scholar
  9. 9.
    Gerstein W (1971) Cell proliferation in human fetal epidermis. J Invest Dermatol 57: 262–265PubMedCrossRefGoogle Scholar
  10. 10.
    Grayson S, Elias PM (1982) Isolation and lipid biochemical characterization of stratum corneum membrane complexes: Implications for the cutaneous permeability barrier. J Invest Dermatol 78: 128–135Google Scholar
  11. 11.
    Härkönen M, Hopsu-Havu VK, Raji K (1974) Cyclic adenosine monophosphate, adenyl cyclase and cyclic nucleotide phosphodiesterase in psoriatic epidermis. Acta Dermatovenereol (Stockh) 54: 13–18Google Scholar
  12. 12.
    Hashimoto K (1971) Cementosome, a new interpretation of the membranecoating granule. Arch Dermatol Forsch 240: 349–364PubMedCrossRefGoogle Scholar
  13. 13.
    Herken R (1977) The influence of D-penicillamine on the proliferation rate of cells from the upper limb bud of mouse embryos in vitro. Teratology 15: 159–161PubMedCrossRefGoogle Scholar
  14. 14.
    Herken R, Schultz-Ehrenburg U (1981) Autoradiographic investigations on the cell kinetics of epidermis and periderm of limb buds from mouse embryos in vitro. Br J Dermatol 104: 277–284PubMedCrossRefGoogle Scholar
  15. 15.
    Herken R, Schultz-Ehrenburg U (1983) Autoradiographic study of glycoprotein synthesis during embryonic epidermogenesis of the mouse in vitro. Arch Dermatol Res 275: 109–113PubMedCrossRefGoogle Scholar
  16. 16.
    Marcelo CL, Duell EA, Stawiski MA, Anderson TF, Voorhees JJ (1979) Cyclic nucleotide levels in psoriatic and normal keratomed epidermis. J Invest Dermatol 72: 20–24PubMedCrossRefGoogle Scholar
  17. 17.
    Neubert D, Merker H-J, Tapken S (1974) Comparative studies on the prenatal development of mouse extremities in vivo and in organ culture. Naunyn-Schmiedeberg’s Arch Pharmacol 286: 251–270CrossRefGoogle Scholar
  18. 18.
    Schultz-Ehrenburg U (1975) Differentiation of the epidermis in the limb bud culture. In: Neubert D, Merker H-J (eds) New approaches to the evaluation of abnormal embryonic development. Georg Thieme, Stuttgart, pp 213–226Google Scholar
  19. 19.
    Schultz-Ehrenburg U (1980) Die Epidermisentwicklung im In-vitro-Modell. Vergleichende licht-, elektronenmikroskopische und autoradiographische Studien an der Extremitätenknospenkultur. Habilitationsschrift, Freie Universität BerlinGoogle Scholar
  20. 20.
    Smart IHM (1970) Variation in the place of cell cleavage during the process of stratification in the epidermis. Br J Dermatol 82: 276–282PubMedCrossRefGoogle Scholar
  21. 21.
    Staak WJBM van de, Stadhouders AM, Gilsing H (1969) A comparative electron microscopic and histochemical investigation of membranecoating granules in normal human skin and in the skin of psoriasis vulgaris patients. Dermatologica 138: 341–345Google Scholar
  22. 22.
    Timson J (1975) Hydroxyurea. Mutat Res 32: 115–132PubMedGoogle Scholar
  23. 23.
    Voorhees JJ, Duell EA, Kelsey WH (1972) Dibutyryl cyclic AMP inhibition of epidermal cell division. Arch Dermatol 105: 384–386PubMedCrossRefGoogle Scholar
  24. 24.
    Voorhees JJ, Duell EA, Bass LJ, Powell JA, Harrell ER (1972) Decreased cyclic AMp in the epidermis of lesion of psoriasis. Arch Dermatol 105: 695–701PubMedCrossRefGoogle Scholar
  25. 25.
    Voorhees JJ, Stawiski M, Duell EA, Haddox MK, Goldberg ND (1973) Increased cyclic GMP and decreased cyclic AMP levels in the hyperplastic, abnormally differentiated epidermis of psoriasis. Life Sci 13: 639–653CrossRefGoogle Scholar
  26. 26.
    Wegener K, Hollweg S, Maurer W (1964) Autoradiographische Bestimmung der DNS-Verdopplung and anderer Teil-Phasen des Zell-Zyklus bei fetalen Zellarten der Ratte. Zeitschr Zellf 63: 309–326CrossRefGoogle Scholar
  27. 27.
    Wessels NK (1963) Effects of extra-epithelial factors on the incorporation of thymidine by embryonic epidermis. Exp Cell Res 30: 36–55CrossRefGoogle Scholar
  28. 28.
    Yoshikawa K, Adachi K, Halprin KM, Levine V (1975) Is the cyclic AMP in psoriatic epidermis low? Br J Dermatol 93: 253–258PubMedCrossRefGoogle Scholar
  29. 29.
    Zimmermann B, Bachmann D, Neubert D, Merker HJ (1975) Induction of skeletal malformations in organ cultures of mouse limb buds. Experientia (Basel) 31: 227–228CrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1986

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  • U. Schultz-Ehrenburg

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