Skin Models pp 199-204 | Cite as

Comparative Study of the Keratin Polypeptide Profiles in Human and in Pig Hyperkeratotic Scabies

  • D. Van Neste
  • M. J. Staquet
  • G. P. Martineau
  • J. P. Ortonne
Conference paper

Abstract

Increased numbers of patients with hyperkeratotic (or Norwegian) scabies have been reported in the literature in recent years. Various environmental and endogeneous factors are known to be associated with the disease [4]. An animal model could be useful to evaluate the relative importance of each of these factors by modifying them one by one and could provide deeper insight into the disturbed keratinization process which is the clinical characteristic feature of this parasitic disease. As there is strong evidence now that fibrous proteins of different physicochemical properties are intimately associated with the epidermal cell differentiation process [2, 19, 20] and that polypeptide composition of the fibrous protein is subjected to environmental influences, we were interested in evaluating the electrophoretic patterns of the fibrous proteins in scales of human and pig hyperkeratotic scabies.

Keywords

Albumin Electrophoresis Polypeptide Trypsin Cyclophosphamide 

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References

  1. 1.
    Ackerman AB (1977) Histopathology of human scabies. In: Orkin O, Maibach HI, Parrish LC, Schwartzman RM (eds) Scabies and pediculosis, Lippincott, Philadelphia, pp 88–95Google Scholar
  2. 2.
    Baden HP, Kubilus J, Argyris TS (1980) Modification of polypeptide composition in keratinocyte fibrous protein. J Invest Dermatol 75: 383–387PubMedCrossRefGoogle Scholar
  3. 3.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254PubMedCrossRefGoogle Scholar
  4. 4.
    Chouvet B, Ortonne JP, Perrot H, Thivolet J (1979) La gale norvégienne: circonstances etiologiques. Ann Dermatol Vénéréol 106: 569–574PubMedGoogle Scholar
  5. 5.
    Fuchs E, Green H (1980) Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19: 1033–1042PubMedCrossRefGoogle Scholar
  6. 6.
    Heilesen B (1946) Studies on acarus scabiei and scabies. Histological examinations of the scabies burrow and the eruption in ordinary scabies. Acta Dermatovenereol (Stockh) 26: suppl 14: 223–240Google Scholar
  7. 7.
    Hunter L, Skerrow D (1982) The proteins of living psoriatic epidermis. Biochim Biophys Acta 714: 164–169PubMedCrossRefGoogle Scholar
  8. 8.
    Karasek J, Oehlert W (1968) Die Ultrastruktur der Epidermis des Schweines. I. Stratum basale und stratum spinosum. Z Mikrosk Anat Forsch 78: 133–144Google Scholar
  9. 9.
    Karasek J, Oehlert W (1968) Die Ultrastruktur der Schweineepidermis. II. Stratum granulo-sum und corneum. Z Mikrosk Anat Forsch 79: 157–169Google Scholar
  10. 10.
    Karcher D, Lowenthal A, Thormar H, Noppe M (1981) Serological identification of viral antigens after electrophoretic transfer. J Immunol Meth 43: 175–179CrossRefGoogle Scholar
  11. 11.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277: 680–685CrossRefGoogle Scholar
  12. 12.
    Löning T, Staquet MK, Thivolet J, Seifert G (1980) Keratin polypeptides distribution in normal and diseased human epidermis and oral mucosa. Virchows Arch A 388: 273–288Google Scholar
  13. 13.
    Sheahan BJ (1975) Pathology of sarcoptes scabiei infection in pigs. I. Naturally occurring and experimentally induced lesions. J Comp Path 85: 87–95PubMedCrossRefGoogle Scholar
  14. 14.
    Shelley WB, Shelley ED (1983) Scanning electron microscopy of the scabies burrow and its contents, with special reference to the sarcoptes scabiei egg. J Am Acad Dermatol 9: 673679Google Scholar
  15. 15.
    Staquet MJ, Viac J, Thivolet J (1981) Keratin polypeptide modifications induced by human papilloma viruses ( HPV ). Arch Dermatol Res 271: 83–90Google Scholar
  16. 16.
    Staquet MJ, Faure M, Reano A, Viac J, Thivolet J (1983) Keratin polypeptide profile in psoriatic epidermis normalized by treatment with etretinate (aromatic retinoid Ro 10–9359). Arch Dermatol Res 275: 124–129PubMedCrossRefGoogle Scholar
  17. 17.
    Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 76: 4350–4354PubMedCrossRefGoogle Scholar
  18. 18.
    Van Neste D, Lachapelle JM (1981) Host-parasite relationships in hyperkeratotic (Norwegian) scabies: pathological and immunological findings. Br J Dermatol 105: 667–678PubMedCrossRefGoogle Scholar
  19. 19.
    Viac J, Schmitt D, Staquet MJ, Thivolet J, Ortonne JP, Bustamante R (1980) Binding specificity of guinea pig anti-a-keratin polypeptide sera on human keratinocytes: comparison of their receptors with those of human epidermal cytoplasmic antibodies. Acta Dermatovenereol (Stockh) 60: 189–196Google Scholar
  20. 20.
    Viac J, Staquet MJ, Thivolet J, Goujon C (1980) Experimental production of antibodies against stratum corneum keratin polypeptides. Arch Dermatol Res 267: 179–188PubMedCrossRefGoogle Scholar
  21. 21.
    Viac J, Reano A, Thivolet J (1982) Cytokeratins in human basal and squamous cell carcinomas: biochemical, immunohistological findings and comparisons with normal epithelia. J Cut Path 9: 377–390CrossRefGoogle Scholar
  22. 22.
    Weinstein GD (1965) Comparison of turnover time and of keratinous protein fractions in swine and human epidermis. In: Bustad LK, McClellan RO (eds) Swine in biomedical research. Frayn Printing Co, Seattle, pp 287–297Google Scholar
  23. 23.
    Winter H, Schweizer J, Goerttler K (1980) Keratins as markers of malignancy in mouse epidermal tumors. Carcinogenesis 1: 391–398PubMedCrossRefGoogle Scholar
  24. 24.
    Winter H, Schweizer J, Goerttler K (1983) Keratin polypeptide composition as a biochemical tool for the discrimination of benign and malignant epithelial lesions in man. Arch Dermatol Res 275: 27–34PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • D. Van Neste
  • M. J. Staquet
  • G. P. Martineau
  • J. P. Ortonne

There are no affiliations available

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