A T Lymphocyte-Derived Differentiation-Inducing Factor for Myeloid Leukemia Cells: Purification and Characterization

  • I. Olsson
  • U. Gullberg
  • M. G. Sarngadharan
  • R. C. Gallo
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 29)


Acute myeloid leukemia (AML) is characterized by an apparent maturation arrest leading to accumulation of immature cells. Such cells obviously preserve normally transient phenotypes. Recent work also indicates that leukemic transformation does not necessarily lead to an irreversible block in cell differentiation. Leukemic cell lines which grow continuously in vitro are useful systems in the search for normal regulatory molecules which can act in leukemia. The promyelocytic HL-60 line [2] is induced into granulocytes and monocytes by incubation with a variety of agents [4]. We have concentrated our work on mechanisms of action of “physiologic” inducers of differentiation in leukemia. Nitroblue tetrazolium (NBT) reduction has been used as the parameter of induced maturation in HL-60 as well as phagocytic capacity, cell surface antigen expression detected with monoclonal antibodies, nonspecific esterase, and composition of cytoplasmic granules.


Acute Myeloid Leukemia Retinoic Acid Leukemic Transformation Charge Heterogeneity Human Mononuclear Blood Cell 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • I. Olsson
  • U. Gullberg
  • M. G. Sarngadharan
  • R. C. Gallo

There are no affiliations available

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