A T Lymphocyte-Derived Differentiation-Inducing Factor for Myeloid Leukemia Cells: Purification and Characterization
Acute myeloid leukemia (AML) is characterized by an apparent maturation arrest leading to accumulation of immature cells. Such cells obviously preserve normally transient phenotypes. Recent work also indicates that leukemic transformation does not necessarily lead to an irreversible block in cell differentiation. Leukemic cell lines which grow continuously in vitro are useful systems in the search for normal regulatory molecules which can act in leukemia. The promyelocytic HL-60 line  is induced into granulocytes and monocytes by incubation with a variety of agents . We have concentrated our work on mechanisms of action of “physiologic” inducers of differentiation in leukemia. Nitroblue tetrazolium (NBT) reduction has been used as the parameter of induced maturation in HL-60 as well as phagocytic capacity, cell surface antigen expression detected with monoclonal antibodies, nonspecific esterase, and composition of cytoplasmic granules.
KeywordsAcute Myeloid Leukemia Retinoic Acid Leukemic Transformation Charge Heterogeneity Human Mononuclear Blood Cell
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