Influence of Colchicine and Cytochalasin B on Pinocytosis, Phagocytosis, and Antibody-Dependent Cell-Mediated Cytotoxicity

  • A. Piasek
  • P. Oblakowski
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 29)


The cytoskeleton is an organelle of eukaryotic cells engaged in Organization and external and internal cell movements, e. g., endocytosis. Our previous experiments proved that colchicine (an antitubular drug) and cytochalasin B (cyt B, an antimicrofilament agent) have significant influence on the development of the process [8, 9]. Reports on the inhibiting effect of the development of antibody-dependent cellmediated cytotoxicity (ADCC) are inconsistent. The aim of the present work was to examine the influence of colchicine and cyt B on the development and mutual dependence of pinocytosis, phagocytosis, and cytotoxic effect.


Hydrogen Peroxide Leukemia Superoxide Bacillus Dition 


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  1. 1.
    Adams DO, Kao KJ, Färb R, Pizzo SU (1980) Effector mechanisms of cytolytically activated macrophages. II. Secretion of a cytolytic factor by activated macrophages and it’s relationship to secreted neutral proteases. J Immunol 124: 239–300Google Scholar
  2. 2.
    Currie GAW (1978) Activated macrophages kill tumor cells by releasing arginase. Nature 273: 758–759PubMedCrossRefGoogle Scholar
  3. 3.
    Fleer A, Van Schaik MLJ, Von Der Borne AEGK, Engelfriet CP (1978) Destruction of sensitized erythrocytes by human monocytes in vitro: effects of cytochalasin B, hydrocortisone and colchicine. Scand J Immunol 8: 515–524PubMedCrossRefGoogle Scholar
  4. 4.
    Graham RC, Karnowsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14: 669–684CrossRefGoogle Scholar
  5. 5.
    Hibbs JB Jr (1974) Heterocytolysis by macrophages activated by Bacillus Calmette- Guerin: lysosomal exocytosis into the tumor cells. Science 184: 468–471PubMedCrossRefGoogle Scholar
  6. 6.
    Johnston RBJ, Godzik CA, Cohn ZA (1978) Increased Superoxide anion production by immunologically activated and chemically elicited macrophages. J Exp Med 148: 115–127PubMedCrossRefGoogle Scholar
  7. 7.
    Nathan C, Cohn Z (1980) Role of oxygendependent mechanisms in antibody-induced lysis of tumor cells by activated macrophages. J Exp Med 152: 198–208PubMedCrossRefGoogle Scholar
  8. 8.
    Piasek A, Thyberg J (1979) Effects of colchicine on endocytosis and cellular inactivation of horseradish peroxidase in cultured chondrocytes. J Cell Biol 81: 426–437PubMedCrossRefGoogle Scholar
  9. 9.
    Piasek A, Thyberg J (1980) Effects of colchicine on endocytosis of horseradish peroxidase by rat peritoneal macrophages. J Cell Sei 45: 59–71Google Scholar
  10. 10.
    Southwick FS, Stossel TP (1983) Contractile proteins in leukocyte function. Sem Hematol 20: 305–320Google Scholar
  11. 11.
    Walker WS, Demus A (1975) Antibody-dependent cytolysis of chicken erythrocytes by an in vitro established line of mouse peritoneal macrophages. J Immunol 114: 765–769PubMedGoogle Scholar
  12. 12.
    Walker WS (1977) Mediation of macrophage cytolytic and phagocytic activities by antibodies of different classes and class-specific Fe receptors. J Immunol 119: 367–373PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • A. Piasek
    • 1
  • P. Oblakowski
    • 2
  1. 1.Department of Histology and EmbryologyInstitute of Biostructure, Medical SchoolWarsawPoland
  2. 2.Department of MedicineInstitute of HaematologyWarsawPoland

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