Leukocyte Counts and Complement Activation During Arteriovenous and Pump-Driven Hemofiltration
Significant leukopenia accompanied by complement activation and impairment of pulmonary function [1, 16] after starting a dialysis treatment were first observed by Kaplow and Goffinet in 1968 . These changes are most striking when cuprophane membranes are used for dialysis . Craddock et al. have studied these side effects of hemodialysis thoroughly and postulate the following pathogenesis: The complement system is activated during contact of the plasma with the dialyzer membrane [3, 4]. C5a fragment of the complement component C5 induces aggregation of granulocytes in the patient’s venous blood [5, 10, 17]. These aggregates are trapped in the pulmonary microvessels resulting in a transient, profound leukopenia of the peripheral blood .
KeywordsLeukocyte Count Complement Activation Cellulose Triacetate Cuprophane Membrane Complement Concentration
Unable to display preview. Download preview PDF.
- 1.Aljama P, Bird PAE, Ward MK, Feest TG, Walker W, Tanboga H, Sussman, M, Kerr DNS (1978) Haemodialysis-induced leukopenia and activation of complement: effects of different membranes. Proc Eur Dial Transpl Ass 15: 144Google Scholar
- 5.Craddock PR, Hammerschmidt D, White JG, Dalmasso AP, Jacob H (1977) Complement ( C5a)-induced granulocyte aggregation in vitro, a possible mechanism of complement-mediated leukostasis and leukopenia. J Clin Invest 60: 260Google Scholar
- 6.Craddock PR, White JG, Weisdorf DJ, Hammerschmidt DE (1980) Digital integration of granulocyte aggregation responses. A simple and reproducible method for the quantification of granulocyte adhesiveness. Inflammation 4: 381Google Scholar
- 8.Graf H, Stummvoll HK, Haber P, Kovarik J (1980) Pathophysiology of dialysis related hypoxemia. Proc Eur Dial Transpl Ass 17: 155Google Scholar
- 11.Hjorth R, Jonsson AK, Vretblad P (1981) A rapid method for purification of human granulocytes using Percoll. A comparison with dextran Sedimentation. J Immunol Methods 43: 95Google Scholar
- 17.Sacks T, Moldow CF, Craddock PR, Bowers TK, Jacob HS (1978) Oxygen radicals mediate endothelial cell damage by complement-stimulated granulocytes. An in vitro model of immune vascular damage. J Clin Invest 61: 1161Google Scholar