The Immunological Significance of Antibody Affinity

  • M. E. Devey
  • M. W. Steward
Conference paper
Part of the Bayer-Symposium book series (BAYER-SYMP, volume 8)


Mice selectively bred to produce low affinity antibodies are more susceptible to immune complex glomerulonephritis induced by daily injections of a foreign protein antigen than are mice selected to produce high affinity antibody. Development of disease is associated with an inability to produce the normal maturational switch from low to high affinity antibody. Low affinity NM mice, selected by their failure to show affinity maturation after injection of adjuvantised antigen showed an even greater susceptibility to disease. Sucrose density gradient ultracentrifugation studies showed that immune complexes formed in high affinity mice were large (19 S) and cleared rapidly from the circulation. Immune complexes in low affinity mice were significantly smaller. We propose that immune complex diseases may result from an inherited or acquired form of immunodeficiency favouring a predominantly low affinity antibody response. Immune complexes formed from low affinity antibody have a tendency to dissociate and form small (12–17 S) complexes in antigen excess which may deposit in filtering membranes such as the renal glomerulus.


Human Serum Albumin Immune Complex Affinity Antibody Renal Glomerulus Immune Complex Formation 
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  1. 1.
    Steward MW, Petty RE (1972) The use of ammonium sulphate globulin precipitation for determination of affinity of anti-protein antibodies in mouse serum. Immunology 22: 474Google Scholar
  2. 2.
    Steward MW, Petty RE (1972) The antigen-binding characteristics of antibody pools of different relative affinity. Immunology 23: 881PubMedGoogle Scholar
  3. 3.
    Stanley C, Lew AM, Steward MW (1983) The measurement of antibody affinity: a comparison of five techniques using a panel of monoclonal anti-DNP antibodies and the effect of high affinity antibody on the measurement of low affinity antibody. J Immunol Methods 64: 119PubMedCrossRefGoogle Scholar
  4. 4.
    Petty RE, Steward MW, Soothill JF (1972) The heterogeneity of antibody affinity in inbred mice and its possible immunopathologic significance. Clin Exp Immunol 12: 231PubMedGoogle Scholar
  5. 5.
    Petty RE, Steward MW (1977) The effect of immunological adjuvants on the relative affinity of antiprotein antibodies. Immunology 32: 49PubMedGoogle Scholar
  6. 6.
    Steward MW, Reinhardt MC, Staines NA (1979) The genetic control of antibody affinity: Evidence from breeding studies with mice selectively bred for either high or low affinity antibody production. Immunology 37: 697PubMedGoogle Scholar
  7. 7.
    Steward MW, Steensgaard (1983) Antibody affinity: Thermodynamic aspects and biological significance. CRC Press, Boca RatonGoogle Scholar
  8. 8.
    Ahlstedt S, Holmgren J, Hanson LA (1974) Protective capacity of antibodies against E. coli O antigen with special reference to the avidity. Int Arch allergy 46: 470PubMedCrossRefGoogle Scholar
  9. 9.
    Soothill JF, Steward MW (1971) The immunopathological significance of the heterogeneity of antibody affinity. Clin Exp Immunol 9: 193PubMedGoogle Scholar
  10. 10.
    Soothill JF, Smith MD, Morgan AG (1975) Immunodeficiency and the allergic effects of parasitic infection. In: Taylor AER, Miller R (eds) Pathogenic processes in parasitic infections, vol 13. Blackwell, Oxford, p 59Google Scholar
  11. 11.
    Katz FE, Steward MW (1976) The genetic control of antibody affinity in mice. Immunology 29: 543Google Scholar
  12. 12.
    Steward MW (1979) Chronic immune complex disease in mice. The role of antibody affinity. Clin Exp Immunol 38: 414PubMedGoogle Scholar
  13. 13.
    Devey ME, Steward MW (1980) The induction of chronic antigen-antibody complex disease in selectively bred mice producing either high or low affinity antibody to protein antigens. Immunology 41: 303PubMedGoogle Scholar
  14. 14.
    Devey ME, Bleasdale K, Collins M, Steward MW (1982) Experimental antigen-antibody complex disease in mice: the role of antibody levels, antibody affinity and circulating antigen-antibody complexes. Int Arch Allergy Appl Immunol 68: 47PubMedCrossRefGoogle Scholar
  15. 15.
    Steward MW, Collins MJ, Stanley C, Devey ME (1981) Chronic antigen-antibody complex glomerulonephritis in mice. Br J Exp Pathol 62: 614PubMedGoogle Scholar
  16. 16.
    Devey ME, Bleasdale K, Stanley C, Steward MW (1984) Failure of affinity maturation leads to increased susceptibility to immune complex glomerulonephritis. Immunology 52: 377PubMedGoogle Scholar
  17. 17.
    Lew AM, Staines NA, Steward MW (1984) Glomerulonephritis induced by preformed immune complexs containing monoclonal antibodies of defined antibody and isotype. Clin Exp Immunol 47: 413Google Scholar
  18. 18.
    Lew AM, Steward MW (1984) Glomerulonephritis: the use of grafted hybridomas to investigate the role of epitope density, antibody affinity and antibody isotype in active serum sickness. Immunology 52: 367PubMedGoogle Scholar
  19. 19.
    Haakenstad AO, Striker GE, Mannik M (1976) The glomerular deposition of soluble immune complexex prepared with reduced and alkylated antibodies and with intact antibodies in mice. Lab Invest 35: 293PubMedGoogle Scholar
  20. 20.
    Germuth FG, Rodriguez CA, Wise OL (1982) Passive immune complex glomerulonephritis in mice III. Clearance kinetics and properties of circulating complexes. Lab Invest 46: 515PubMedGoogle Scholar
  21. 21.
    Couser WG, Salant DJ (1980) In situ immune complex formation and glomerular injury. Kidney Int 17: 1PubMedCrossRefGoogle Scholar
  22. 22.
    Lew AM, Tovey DG, Steward MW (1984) Localisation of covalent immune complexes on the epithelial side of the glomerular basement membrane in mice. Int Arch Allerg Appl Immunol 75: 242CrossRefGoogle Scholar
  23. 23.
    Reinhardt MC, Steward MW (1979) Antibody affinity and clearance function studies in high and low antibody affinity mice. The effect of protein defificiency. Immunology 38: 735PubMedGoogle Scholar
  24. 24.
    Steward MW, Voller A (1973) The effect of malaria on the relative affinity of mouse anti protein antibody. Br J Exp Pathol 54: 198PubMedGoogle Scholar
  25. 25.
    Pattison JR, Steward MW, Target GAT (1983) Trypanosome infection depresses antibody affinity and delays affinity maturation. Clin Exp Immunol 53: 175PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • M. E. Devey
  • M. W. Steward

There are no affiliations available

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