Skip to main content
SpringerLink
Log in

Critical Evaluation of Methods for Estimating the Vacuolar pH of Plant Cells

  • Conference paper
Book cover Biochemistry and Function of Vacuolar Adenosine-Triphosphatase in Fungi and Plants

Abstract

The vacuoles of higher plant cells have been shown to be the site for accumulation of ions and metabolites (Matile 1978; Leigh et al. 1979, Leigh 1983; Marty et al. 1980; Boudet et al. 1984). The mechanisms involved in the storage functions of vacuoles are not well known. However, the presence of ATPases on the tonoplast of a number of plant species has been demonstrated (d’Auzac 1977; Lin et al. 1977 ; Leigh and Walker 1980; Cretin 1982; Aoki and Nishida 1984). These ATPases are considered to be responsible for creating a proton electrochemical gradient across the tonoplast. A classical idea is that this gradient could be used for the transport of ions and metabolites and their accumulation in the vacuole. The proton gradient across the tonoplast can also be directly used for the accumulation of secondary plant products diffusing through membranes as neutral molecules and accumulated as cations. This is the case for some indole alkaloids (Renaudin and Guern 1982) and nicotine (Kurkdjian 1982).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aducci P, Federico R, Carpinelli G, Podo F (1982) Temperature dependence of intracellular pH in higher plant cells. Planta 156: 579–582

    Article  CAS  Google Scholar 

  • Alibert G, Carrasco A, Boudet AM (1982) Changes in biochemical composition of vacuoles isolated from Acer pseudoplatanus L. during cell culture. Biochim Biophys Acta 721: 22–29

    Article  CAS  Google Scholar 

  • d’Auzac J (1977) ATPase membranaire de vacuoles lysosomales: les lutoi’des du latex d’Hevea brasiliensis. Phytochem 16: 1881–1885

    Article  Google Scholar 

  • Ammann D, Lanter F, Steiner RA, Schulthess P, Shijo Y, Simon W (1981) Neutral carrier based hydrogen ion-selective microelectrode for extra and intracellular studies. Anal Chem 53: 2267–2269

    Article  PubMed  CAS  Google Scholar 

  • Aoki K, Nishida K (1984) ATPase activity associated with vacuoles and tonoplast vesicles isolated from the CAM plant, Kalanchoe daiqremontiana. Physiol Plantarum 60: 21–24

    Article  CAS  Google Scholar 

  • Boudet AM, Alibert G, Marigo G (1984) Vacuoles and tonoplast in regulation of cellular metabolism. In: Alibert G, Boudet AM, Lea PJ (eds). Annu Proc of the Phytochem Soc of Europe : Membranes and compartmentation in the regulation of plant function, vol 23 Oxford University press, (in press)

    Google Scholar 

  • Caldwell PD (1956) Intracellular pH. Internat Rev Cytol 5: 229–277

    Article  CAS  Google Scholar 

  • Chow WS, Barber J (1980) 9-aminoacridine fluorescence changes as a measure of surface Charge density of the thylakoid membrane. Biochim Biophys Acta 589: 346–352

    Article  PubMed  CAS  Google Scholar 

  • Crétin H, (1982) The proton gradient across the vacuo- lysosomal membrane of lutoids from the latex of Hevea brasiliensis. I. Further evidence for a proton-translocating ATPase on the vacuolysosomal membrane of intact lutoids. J Membrane Biol 65: 175–184

    Article  Google Scholar 

  • Foyer C, Walker D, Spencer C, Manu B (1982) Observations on the phosphate status and intracellular pH of intact cells, protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance. Biochem J 202: 429–434

    PubMed  CAS  Google Scholar 

  • Kubota Y, Modota Y (1980) Nanosecond fluorescence decay studies of the deoxyribonucleic acid- 9-aminoacridine and deoxyribonucleic acid-9amino-10-methylacridinium complexes. Biochem 19: 4189–4197

    Article  CAS  Google Scholar 

  • Kurkdjian A (1982) Absorption and accumulation of nicotine by Acer pseudoplatanus and Nicotiana tabacum cells. Physiol vég 20: 73–83

    CAS  Google Scholar 

  • Kurkdjian A, Barbier-Brygoo H (1983) A hydrogen ion-selective Liquid-membrane microelectrode for measurement of the vacuolar pH of plant cells in suspension culture. Anal Biochem 132: 96–104

    Article  PubMed  CAS  Google Scholar 

  • Kurkdjian A, Barbier-Brygoo H, Manigault J, Manigault P (1984) Distribution of vacuolar pH values within populations of cells, protoplasts and vacuoles isolated from suspension cultures and plant tissues. Physiol veg 22: 193–198

    Google Scholar 

  • Kurkdjian A, Guern J (1981) Vacuolar pH measurement in higher plant cells. I. Evaluation of the methylamine method. Plant Physiol 67: 953–957

    Google Scholar 

  • Kurkdjian A, Mathieu Y, Guern J (1982) Evidence for an action of 2,4-dichlorophenoxyacetic acid on the vacuolar pH of Acer pseudoplatanus cells in suspension culture. Plant Sei Lett 27: 77–86

    Article  CAS  Google Scholar 

  • Lee RB, Ratcliffe RG (1983) Development of an aeration system for use in plant tissue NMR experiments. J Exp Bot 34: 1213–1221

    Article  CAS  Google Scholar 

  • Leigh RA (1983) Methods, progress and potential for the use of isolated vacuoles in studies of solute transport in higher plant cells. Physiol Plant 57: 390–396

    Article  CAS  Google Scholar 

  • Leigh RA, Rees T, Fuller AW, Banfield J (1979) The location of acid invertase activity and sucrose in the vacuoles of storage roots of beetroot (Beta vulgaris). Biochem J 178: 539–547

    PubMed  CAS  Google Scholar 

  • Leigh RA, Walker RR (1980) ATPase and acid phosphatase activities associated with vacuoles isolated from storage roots of red beet (Beta vulgaris L.) Planta 150: 222–229

    CAS  Google Scholar 

  • Lin W, Wagner GJ, Siegelman HW, Hind G (1977) Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue. Biochim Biophys Acta 465: 110–117

    Article  PubMed  CAS  Google Scholar 

  • Lüttge U, Ball E (1980) 2H+: 1 malate2 stoichiometry during crassulacean acid metabolism is unaffected by lipophilic cations. Plant Cell Environment 3: 195–200

    Google Scholar 

  • MacFarlane JJ, Smith FA (1982) Uptake of methylamine by Ulva rigida: transport of cations and diffusion of free base. J Exp Bot 33: 195–207

    Article  CAS  Google Scholar 

  • Manigault P, Manigault J, Kurkdjian A (1983) A microfluorimetric method for vacuolar pH measurement in plant cells using 9-aminoacridine. Physiol veg 21: 129–136

    CAS  Google Scholar 

  • Marigo G, Ball E, Lüttge U, Smith AC (1982) Use of the DMO technique for the study of relative changes of cytoplasmic pH in leaf cells in relation to CAM. Z Pflanzenphysiol 188: 223–233

    Google Scholar 

  • Marigo G, De Lorme YM, Lüttge U, Boudet AM (1983) Role de l’acide malique dans la regulation du pH vacuolaire dans des cellules de Catharanthus roseus cultivees in vitro. Physiol veg 21: 1135–1144

    CAS  Google Scholar 

  • Martin JB, Bligny R, Rebeil1e F, Douce R, Leguay JJ, Mathieu Y, Guern J (1982) A 31P NMR study of intracellular pH of plant cells cultivated in liquid medium. Plant Physiol 70: 1156–1161

    Article  PubMed  CAS  Google Scholar 

  • Marty F, Branton D, Leigh RA (1980) Plant vacuoles. In: Tolbert (ed) The Biochemistry of plants, vol. 1. The plant cell. Acad. Press New-York, p 625

    Google Scholar 

  • Marty A, Viallet P (1979) Etüde de l’inhibition de la fluorescence de 1’amino-9-acridine et de 1’amino-9chloro-6 acridine par la serumalbumine humaine. C R Acad Sei Paris 288: 1715–1718

    CAS  Google Scholar 

  • Matile P (1978) Biochemistry and function of vacuoles. Ann Rev Plant Physiol 29: 193–213

    Article  CAS  Google Scholar 

  • Murphy TM, Matson GB, Morrison SL (1983) Ultraviolet-stimulated KHCO3 efflux from Rose cells. Plant Physiol 73: 20–24

    Article  PubMed  CAS  Google Scholar 

  • Nishimura M (1982) pH in vacuoles isolated from castor bean endosperm. Plant Physiol 70:742–744

    Google Scholar 

  • Raven JA, Farquhar GD (1981) Methylammonium transport in Phaseolus vulgaris leaf slices. Plant Physiol 67: 859–863

    Article  PubMed  CAS  Google Scholar 

  • Renaudin JP, Guern J (1982) Compartmentation mechanisms of indole alkaloids in cell suspension cultures of Catharanthus roseus. Physiol vég 20: 533–547

    CAS  Google Scholar 

  • Roberts JKM, Ray PM, Wade-Jardetzky N, Jardetzky O (1980) Estimation of cytoplasmic and vacuolar pH in higher plant cells by 31P NMR. Nature 283: 870–872

    Article  CAS  Google Scholar 

  • Roberts JKM,Ray PM, Wade-Jardetzky N, Jardetzky O (1981a) Extent of intracellular pH changes during H+ extrusion by maize root-tip cells. Planta 152: 74–78

    Google Scholar 

  • Roberts JKM, Wade-Jardetzky N, Jardetzky O (1981b) Intracellular pH measurements by 1 p Nuclear Magnetic Resonance. Influence of factors other than pH on P chemical shifts. Biochemistry 20: 5389–5394

    Google Scholar 

  • Roberts JKM, Wemmer D, Ray PM, Jardetzky O (1982) Regulation of cytoplasmic and vacuolar pH in maize root tips under different experimental conditions. Plant Physiol 69: 1344–1347

    Article  PubMed  CAS  Google Scholar 

  • Romani G, Marre MT, Marre E (1983) Effects of permeant weak acids on dark CO2 fixation and malate level in maize root segments. Physiol veg 21: 867–873

    CAS  Google Scholar 

  • Schibeci A, Henry RJ, Stone BA, Brownlee RTC (1983) 31P NMR measurement of cytoplasmic and vacuolar pH in endosperm cells of ryegrass ( Lolium multiflorum) grown in suspension culture. Biochem Int 6: 837–844

    Google Scholar 

  • Theuvenet APR, Van der Wijngaard WMH, Borst-Pauwels WFH (1983) 9-aminoacridine, a fluorescent probe of the thiamine carrier in yeast cells. Biochim Biophys Acta 730: 255–262

    Google Scholar 

  • Viatagliano V (1983) The aggregation of dyes on polyelectrolytes. In: Wyn-Jones E, Gormally J (eds) Aggregation processes in Solution. Elsevier, Amsterdam Oxford New-York, p271

    Google Scholar 

  • Waddell WJ, Butler TC (1959) Calculation of intracellular pH from the distribution of 5,5 dimethyl 2,4-oxazolidine dione. Application to skeletal muscle of the dog. J Clin Invest 38: 720–729

    Google Scholar 

  • Wagner GJ, Lin W (1982) An active proton pump of intact vacuoles isolated from tulipa petals. Biochim Biophys acta 689: 261–266

    Article  CAS  Google Scholar 

  • Weigel HJ, Weis E (1984) Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of 9-aminoacridine fluorescence. Plant Sei Lett 33: 163–175

    Article  CAS  Google Scholar 

  • Wheeler PA, Hellebust JA (1981) Uptake and concentration of alkylamines by a marine diatom. Effects of H+ and K+ and implications for the transport and accumulation of weak bases. Plant Physiol 67: 367–372

    Article  PubMed  CAS  Google Scholar 

  • Wright SA, Syrett PJ (1983) The uptake of methylammonium and dimethylammonium by the diatom, Phaedactylum tricornutum. New Phytoi 95: 189–202

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Physiologie cellulaire végétale, C.N.R.S.-I.N.R.A., 91190, Gif-sur-Yvette, France

    A. Kurkdjian, H. Barbier-Brygoo, P. Manigault & J. Guern

  2. Laboratoire des Sciences du Sol, C.N.R.A., 78000, Versailles, France

    H. Quiquampoix

  3. Centre de Recherches de la Croix-de-Berny, Rhône-Poulenc Santé, 92160, Antony, France

    M. Péan

Authors
  1. A. Kurkdjian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Quiquampoix
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Barbier-Brygoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Péan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Manigault
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. Guern
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Antenne O.R.S.T.O.M. Service de Radio-Agronomie, Centre d’Etudes Nucléaires de Cadarache, Boîte Postale No. 1, F-13115, Saint-Paul-Lez-Durance, France

    Bernard P. Marin

  2. Bayreuth, Germany

    Bernard P. Marin

  3. Paris, France

    Bernard P. Marin

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Kurkdjian, A., Quiquampoix, H., Barbier-Brygoo, H., Péan, M., Manigault, P., Guern, J. (1985). Critical Evaluation of Methods for Estimating the Vacuolar pH of Plant Cells. In: Marin, B.P. (eds) Biochemistry and Function of Vacuolar Adenosine-Triphosphatase in Fungi and Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70320-1_9

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-70320-1_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70322-5

  • Online ISBN: 978-3-642-70320-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation