Transport and Energization in Vacuoles of Sugarcane

  • E. Komor
  • M. Thom


The storage of sucrose in sugarcane had been of importance for thousands of years since Papua in New Guinea had selected Saccharum species for sweetness. Scientists of the nineteenth Century were also fascinated by the feature that sugarcane can accumulate sucrose up to 20% of fresh weight (Went, 1896). The physiology of sugar storage was especially elucidated by the Australian groups of Bieleski, Hatch and Glasziou. They followed the path of sucrose to the storage parenchyma, the hydrolysis to hexoses, the uptake of hexoses and the resynthesis and storage of sucrose (e.g. Gayler and Glasziou, 1972). A detailed kinetic study of storage processes was then made possible by the successful generation of sugarcane cell culture in suspension (Nickeil and Maretzki 1969) and the kinetic studies revealed that sugar uptake into the storage cells proceeds by strictly coupled proton symport with hexoses (Komor et al. 1981). By that time several procedures for isolation of vacuoles had been described (Leigh et al. 1979) and the successful isolation of intact vacuoles from sugarcane allowed more detailed studies on sugar storage (Thom et al. 1982a).


Sugar Transport Sugar Uptake Sucrose Transport Storage Parenchyma Detailed Kinetic Study 
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  1. Archbold HK (1940) Fructosans in the monocotyledons. A review. New Phytol. 39: 185–219CrossRefGoogle Scholar
  2. Doli S, Rodler F, Willenbrink J (1979) Accumulation of sucrose in vacuoles Isolated from red beet tissue. Planta 144: 407–411CrossRefGoogle Scholar
  3. Doli S, Effelsberg U, Willenbrink J (1982) Characteristics of the sucrose uptake system of vacuoles isolated from red beet tissue. Relationships between ion gradients and sucrose transport. In: Marme D, Marre E, Hertel R (eds) Plasmalemma and tonoplast: their functions in the plant cell. Elsevier Biomedical, Amsterdam, p 217–224Google Scholar
  4. Fisher DB, Outlaw WH (1979) Sucrose compartmentation in the palisade parenchyma of Vicia faba L. Plant Physiol. 64: 481–483PubMedCrossRefGoogle Scholar
  5. Gayler KR, Glasziou KT (1972) Sugar accumulation in sugarcane. Carrier-mediated active transport of glucose. Plant Physiol. 49: 563–568PubMedCrossRefGoogle Scholar
  6. Guy M, Reinhold L, Michaeli D (1979) Direct evidence for a sugar transport mechanism in isolated vacuoles. Plant Physiol. 64: 61–64PubMedCrossRefGoogle Scholar
  7. Kaiser G, Heber U (1984) Sucrose transport into vacuoles isolated from barley mesöphyll protoplasts. Planta (in print)Google Scholar
  8. Kaiser G, Martinoia E, Wiemken A (1982) Rapid appearance of photosynthetic products in the vacuoles isolated from barley mesophyll protoplasts by a new fast method. Z Pfl Physiol 107: 103–113Google Scholar
  9. Knuth Me, Keith B, Clark C, Garcia-Martinez JC, Rappaport L (1983) Stabilization and transport capacity of cowpea and barley vacuoles. Plant Cell Physiol 24: 423–432Google Scholar
  10. Komor E (1982) Transport of sugar. In: Loewus F, Tanner W (eds) Encyl Plant Physiol New Ser Vol 13A. Springer, Berlin-Heidelberg, p 635–676Google Scholar
  11. Komor E, Thom M, Maretzki A (1981) The mechanism of sugar uptake by sugarcane suspension cells. Planta 153:181–192Google Scholar
  12. Komor E, Thom M, Maretzki A (1982) Vacuoles from sugarcane suspension 12. cultures. III Protonmotive potential difference. Plant Physiol 69:1326–1330Google Scholar
  13. Leigh RA (1983) Methods, progress and potential for the use of isolated vacuoles in studies of solute transport in higher plant cells. Physiol Plant 57: 390–396CrossRefGoogle Scholar
  14. Leigh R, Branton D, Marty F (1979) Methods for the isolation of intact vacuoles and fragments of tonoplast. In Plant Organelles, Reid E (ed) Ellis Horwood Ltd, Chichester, p 69–80Google Scholar
  15. Moskowitz AH, Hrazdina G (1981) Vacuolar contents of fruit subepidermal cells from Vitis species. Plant Physiol 68: 686–692PubMedCrossRefGoogle Scholar
  16. Nickell L, Maretzki A (1969) Growth of suspension cultures of sugarcane cells in chemically defined media. Physiol Plant 22: 117–125CrossRefGoogle Scholar
  17. Thom M, Maretzki A, Komor E (1982a) Vacuoles from sugarcane suspension cultures. I Isolation and partial characterization. Plant Physiol 69: 1315-1319Google Scholar
  18. Thom M, Komor E, Maretzki A (1982b) Vacuoles from sugarcane suspension cultures. II Characterization of sugar uptake. Plant Physiol 69: 1320-1324Google Scholar
  19. Thom M, Komor E (1984a) Role of the ATPase of sugar-cane vacuoles in energization of the tonoplast. Eur J Biochem 138: 93–99PubMedCrossRefGoogle Scholar
  20. Thom M, Komor E (1984b) H+-sugar antiport as mechanism of sugar uptake by sugarcane vacuoles. FEBS-Ls (submitted)Google Scholar
  21. Wagner GJ (1979) Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanine in protoplasts Plant Physiol 64: 88–93PubMedCrossRefGoogle Scholar
  22. Wagner W, Keller F, Wiemken A (1983) Fructan metabolism in cereals: Induction in leaves and compartmentation m protoplasts and vacuoles. Z Pfl Physiol 122: 359-372Google Scholar
  23. Went FAFC (1896) Chemisch-physiologische Untersuchungen über das Zuckerrohr. Jahrbuch wiss. Bot 31: 289–344Google Scholar
  24. Willenbrink J, Doli S (1979) Characteristics of the sucrose uptake system of vacuoles from red beet tissue. Kinetics and specificity of the sucrose uptake system. Planta 147: 159-162Google Scholar
  25. Yamaki S (1984) Isolation of vacuoles from immature apple fruit flesh and compartmentation of sugars, organic acids, phenolic Compounds and amino acids. Plant Cell Physiol 25: 151–166Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • E. Komor
    • 1
  • M. Thom
    • 2
  1. 1.Lehrstuhl PflanzenphysiologieUniversität BayreuthBayreuthGermany
  2. 2.Experiment StationHawaiian Sugar Planters’ Assoc.AieaUSA

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