H+-ATPase and H+/Ion Antiporters of the Vacuolar Membrane of Saccharomyces Carlsbergensis Yeast
Various solutes are accumulated by yeast vacuoles (Matile 1978; Okorokov et al. 1980). It is suggested that transport systems of the vacuolar membrane create ion gradients across the yeast tonoplast (Okorokov et al. 1980) and regulate ion concentrations in the cytosol (Lichko et al. 1982). Nowadays there exist convincing evidences that the yeast tonoplast is equipped with the specific ATPase (Okorokov et al. 1982) operating as a proton pump (Kakinuma et al. 1981; Okorokov and Lichko 1983). Ohsumi and Anraku have been the first to present evidences that vesicles of the vacuolar membrane of S. cerevisiae possess arginine/H+ and Ca2+/H+ antiporters (Ohsumi and Anraku 1981; 1983).
KeywordsPermeability Hydrolysis Magnesium Glycerol Adenosine
Unable to display preview. Download preview PDF.
- Kakinuma Y, Ohsuma Y, Anraku Y (1981) Properties of H+-translocating adenosine triphosphatase in vacuolar membrane of Saccharomyces cerevisiae. J Biol Chem 258: 10859–10863Google Scholar
- Lichko LP, Okorokov LA (to be publistied) Some properties of membrane-bound, solubilized and reconstituted in liposomes H+-ATPase of vacuoles of Saccharomyces carlsbergensis. FEBS LettGoogle Scholar
- Okorokov LA, Kulakovskaya TV, Lichko LP, Polorotova EV (to be published) H+/ion antiport is a base mechanism of the functioning of transport systems of vacuolar membrane of Saccharomyces carlsbergensis yeast. FEBS LettGoogle Scholar
- Walker RR, Leigh RA (1981) Characterization of a salt stimulated ATPase activity associated with vacuoles isolated from storage roots of red beet (Beta vulgaris L.) Planta 153: 140–149Google Scholar