Zusammenfassung
In immunhistologischen Untersuchungen an über 100 kryo-konservierten Gewebeproben maligner und nicht-maligner Herkunft konnte die einzigartige Spezifität des monoklonalen GD3-Gangliosid-Antikörpers mAk R-24 für maligne Melanomzellen etabliert werden. Dieser Antikörper (Ak) reagierte mit allen Tumorproben von 21 Patienten mit primärem malignen Melanom und von 20 Patienten mit metastatischem malignen Melanom. Im Gegensatz zu den Gewebeproben beim primären malignen Melanom zeigten Gewebeproben von Patienten mit metastatischem malignen Melanom im mehreren Fällen eine heterogene Anfärbung, selbst bei Metastasen, die vom gleichen Patienten gewonnen wurden. Normale Melanozyten in Hautpräparaten reagierten nicht mit mAk R-24. Normalgewebe unterschiedlicher Herkunft (über 50 Gewebeproben) zeigten ebenfalls keine positive Anfärbung bis auf vereinzelte Zellen (< 1%) in der Nebenniere und im Bindegewebe.
Die Spezifität des monoklonalen Antikörpers mAk R-24 wurde in einer ersten klinischen Anwendung bei 3 Patienten bestätigt. Es kam zu einer Entzündungsreaktion im Tumorbereich. Nebenwirkungen dieser Antikörpergabe wurden nicht beobachtet.
Summary
More than 100 cryopreserved tissue probes of malignant and non-malignant origin were studied by indirect immunoperoxidase staining with monoclonal antibody mAk R-24. This antibody recognizes the disialoganglioside GD3. A striking specificity of this mAk R-24 for malignant melanoma tissue was established. This antibody reacted with all tumor probes of 22 patients with primary malignant melanoma and of 20 patients with metastatic malignant melanoma. More than 80% of tumor cells stained positive in the probes of primary maUgnant melanoma. In contrast melanoma cells in tissue sections of patients with metastatic malignant melanoma showed a more heterogenous staining pattern, even in different metastatic sites of the same patient. Normal melanocytes of human skin did not react with antibody R-24 as well as more than 50 normal human tissue samples of different origin. Only singular cells (< 1%) in the zona reticularis of the pararenal gland and in connective tissue gave positive staining.
The specificity of the mAk R-24 was confirmed in a first clinical application. A local inflammatory response at the tumor site was observed in all 3 patients who had received mAk R-24. No side effects of this mAk-application were observed.
Diese Arbeit wurde gefördert durch Mittel der Deutschen Forschungsgemeinschaft (Di 245/3-2, Kn 180/3-2)
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literatur
Cahan LD, Me RF, Singh R, Cassidenti A, Paulson JC (1982) Identification of a human neuroectodermal tumor antigen (OFA-I-2) as ganglioside GD2. Proc Natl Acad Sci USA 79: 7629–7633
Dippold WG, Lloyd KO, Li LTC, Ikeda H, Oettgen HF, Old LJ (1980) Cell surface antigens of human malignant melanoma: definition of six antigenic systems with monoclonal antibodies. Proc Natl Acad Sci USA 77: 6114–6118
Dippold WG, Knuth A, Meyer zum Büschenfelde KH (1984) Inhibition of human melanoma cell growth in vitro by monoclonal anti-GD3-ganglioside antibody. Cancer Res 44: 806–810
Dippold WG, Knuth A, Meyer zum Büschenfelde KH (1984) Inflammatory response at the tumor site after systemic application of monoclonal anti-GD3-ganglioside antibody to patients with malignant melanoma. Proc Am Assoc Cancer Res 25: 978
Irie RF, Sze LL, Saxton RE (1982) Human antibody to OFA-I, a tumor antigen, produced in vitro by Epstein-Barr virus-transformed human B-lymphoid cell lines. Proc Natl Acad Sci USA 79: 5660–5670
Knuth A, Dippold WG, Houghton AN, Meyer zum Büschenfelde KH, Oettgen HF, Old LJ (1984) ADCC reactivity of human melanoma cells with mouse monoclonal antibodies. Proc Am Assoc Cancer Res 25: 1005
Lloyd KO (1983) Human tumor antigens: detection and characterization with monoclonal antibodies. In: Herberman RB (ed) Basic and Clinical Tumor Immunology, Vol I, S 159–214. Martinus Nijhof Publ, Boston USA
Pukel CS, Lloyd KO, Travassos LR, Dippold WG, Oettgen HF, Old LJ (1982) GD3 - a prominent ganglioside of human melanoma: detection and characterization by mouse monoclonal antibody. J Exp Med 155: 1133–1147
Stein H, Gerdes I, Schwab U, Lemke H, Mason D, Ziegler A, Schienle W, Diehl V (1982) Identification of Hodgkin and Sternberg-Reed cells as a unique cell type derived from a newly detected small cell population. Int J Cancer 30: 445–459
Vogel CW, Welt SW, Carswell EA, Old LJ, Müller-Eberhard HJ (1983) A murine IgG 3 monoclonal antibody to a melanoma antigen that activates complement in vitro and in vivo. Immunobiol 164: 309
Watanabe T, Pukel CS, Takeyama H, Lloyd KO, Shiku H, Li LTC, Travassos LR, Oettgen HF, Old LJ (1982) Human melanoma antigen AH is an autoantigenic ganglioside related to GD2. J Exp Med 156: 1884–1889
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Springer-Verlag Berlin · Heidelberg
About this paper
Cite this paper
Dippold, W.G., Dienes, HP., Knuth, A., Meyer zum Büschenfelde, KH. (1985). Monoklonale Antikörper beim malignen Melanom. In: Holzmann, H., Altmeyer, P., Hör, G., Hahn, K. (eds) Dermatologie und Nuklearmedizin. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70279-2_18
Download citation
DOI: https://doi.org/10.1007/978-3-642-70279-2_18
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-15170-8
Online ISBN: 978-3-642-70279-2
eBook Packages: Springer Book Archive