Some Physiological Properties of Protoplasts from Gravireacting Maize Roots

  • P. E. Pilet
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Protoplasts, with their exposed plasmalemma, have been used in studies of membrane integrity (Fowke et al. 1983). It has been clearly shown that they respond to auxin (such as indole-3-acetic acid: IAA) by increasing their rate of vacuolation until they ultimately burst (>Cocking 1962, Pilet 1971b, 1981). Such plasmalemma disruption, induced by IAA, may depend on the endogenous auxin level of the cells from which protoplasts have been obtained. If onion roots were treated with IAA before the preparation of protoplasts which were then incubated in medium containing IAA, the protoplasts burst at a higher IAA concentration (Pilet 1971b, 1981). Similarly, when using mesophyll protoplasts obtained from two species of tobacco and two of their tumor-prone interspecific hybrids (having an auxin level about 10 to 30 times higher than the two initial species) the bursting response of protoplasts showed that protoplasts from the tumorous hybrid tolerated auxin levels up to 30 times higher than did protoplasts from parent plants (Bayer 1973). It seems clear that protoplasts burst only after exogenous IAA reaches a level comparable with that of endogenous IAA present in the cells from which they were prepared (Pilet 1972, Hall and Cocking 1974, Pilet 1981).


Zeta Potential Maize Root Mesophyll Protoplast Auxin Level Negative Zeta Potential 
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  1. Ambrose EJ (1966) Electrophoretic behaviour of plant cells. Progr Biophys Mol Biol 16:243–265CrossRefGoogle Scholar
  2. Audus LJ (1975) Geotropism in roots. In: Torrey JG, Clarkson DT (eds) The development and Function of roots. Academic, New York, pp 327–363Google Scholar
  3. Bayer MH (1973) Response of Nicotiana mesophyll protoplasts of normal and tumorous origin to indoleactic acid in vitro. Plant Physiol (Bethesda) 51:898–901CrossRefGoogle Scholar
  4. Beffa R, Pilet PE (1982) Elongation and gravireaction of intact and segment roots: light effects. Physiol Plant 54:1–6CrossRefGoogle Scholar
  5. Brinton CC, Lauffer MA (1959) The electrophoresis of viruses, bacteria and cells and the microscope method of electrophoresis. In: Bier M (ed) Electrophoresis: theory, methods and applications I. Academic, London, pp 427–492Google Scholar
  6. Cocking EC (1962) Action of growth substances, chelating agents and antibiotics on isolated root protoplasts. Nature (Lond) 193:998–999CrossRefGoogle Scholar
  7. Cocking EC (1972) Plant cell protoplasts. Isolation and development. Annu Rev Plant Physiol 23:29–50CrossRefGoogle Scholar
  8. Cook GMW, Heard DH, Seaman GVF (1962) The electrokinetic characterization of the Ehrlich carcinoma cells. Exp Cell Res 28:27–39PubMedCrossRefGoogle Scholar
  9. Fowke LC, Griffing LR, Mersey BG, Van der Valk P (1983) Protoplasts for studies of the plasma membrane and associated cell organelles. In: Potrikus I et al. (eds) Protoplasts 1983, Poster Proc 6th Int Protoplast Symp Basel. Birkhäuser Basel, pp 101–118Google Scholar
  10. Grout BWW, Willison JHM, Cocking EC (1972) Interaction at the surface of plant cell protoplasts. Bioenerg 4:585–602Google Scholar
  11. Hall MD, Cocking EC (1974) The response of isolated Avena coleoptile protoplasts to indol-3-acetic acid. Protoplasma 79:225–234PubMedCrossRefGoogle Scholar
  12. Haydon DA (1961) The surface charge of cells and some others small particles as indicated by electrophoresis. The Zeta potential surface charge relationships. Biochim Biophys Acta 50:450–457CrossRefGoogle Scholar
  13. Heard DH, Seaman GVF (1960) The influence of pH and ionic strength on the electrokinetic stability of the human erythrocyte membrane. J Gen Physiol 43:635–654PubMedCrossRefGoogle Scholar
  14. Hertel R, Thomson KS, Russo VEA (1972) In vitro auxin binding to particulate cell fractions from corn coleoptiles. Planta (Berl) 107:325–340CrossRefGoogle Scholar
  15. Jackson MB, Barlow PW (1981) Root geotropism and the role of growth regulators from the cap: a re-examination. Plant Cell Environ 4:107–123CrossRefGoogle Scholar
  16. Kende H, Gardner G (1976) Hormone binding in plants. Annu Rev Plant Physiol 27:267–290CrossRefGoogle Scholar
  17. Moloney MM, Pilet PE (1981) Auxin binding in roots: a comparison between maize roots and coleoptiles. Planta (Berl) 153:447–452CrossRefGoogle Scholar
  18. Nagata T, Melchers G (1978) Surface charge of protoplasts and their significance on cell interaction. Planta (Berl) 142:235–238CrossRefGoogle Scholar
  19. Pilet PE (1971a) Rôle de l’apex radiculaire dans la croissance, le géotropisme et le transport des auxines. Bull Soc Bot Suisse 81:52–65Google Scholar
  20. Pilet PE (1971b) Effets de quelques auxines sur les protoplastes racinaires. CR Acad Sci Paris 273:2253–2256Google Scholar
  21. Pilet PE (1972) Propriétés osmotiques de protoplastes racinaires, mécaniquement et enzymati-quement préparés. Action de traitements auxiniques. CR Acad Sci Paris 275:43–46Google Scholar
  22. Pilet PE (1973) Effect of cell-wall degrading enzymes on protoplast transaminase activity. Ex-perientia (Basel) 29:29–50Google Scholar
  23. Pilet PE (1977) Growth inhibitors in growing and geostimulated maize roots. In: Pilet PE (ed) Plant growth regulation. Springer, Berlin Heidelberg New York, pp 115–128CrossRefGoogle Scholar
  24. Pilet PE (1981) Root protoplasts: some physiological properties. In: Göring H, Hoffmann H (eds) Pflanzliche Gewebekultur. Coll Pflanzenphysiol 4. Humboldt Univ Berlin, pp 35–42Google Scholar
  25. Pilet PE (1984) Auxin effect on protoplasts from gravireacting maize roots. Z Pflanzenphysiol 113:373–376Google Scholar
  26. Pilet PE, Elliott MC (1981) Some aspects of the control of root growth and georeaction: the involvement of indoleacetic acid and abscisic acid. Plant Physiol (Bethesda) 67:1047–1050CrossRefGoogle Scholar
  27. Pilet PE, Ney D (1981) Differential growth of georeacting maize roots. Planta (Berl) 151:146–150CrossRefGoogle Scholar
  28. Pilet PE, Senn A (1974) Effet du Ca2 + et du K+ sur la mobilité électrophorétique des protoplastes. CR Acad Se Paris 278:269–272Google Scholar
  29. Pilet PE, Prat R, Roland JC (1972) Morphology, RNase and transaminase of root protoplasts. Plant Cell Physiol 13:297–309Google Scholar
  30. Pilet PE, Hervé R, Senn A (1984) Zeta potential of protoplasts from gravireacting maize roots. Planta (Berl)Google Scholar
  31. Röder C, Cook GMW, Haemmerli G, Sträuli P (1973) Electrokinetic characterization of two ascites tumours: TA3 and Mel 1. J Cell Sci 12:235–251Google Scholar
  32. Rubery T (1981) Auxin receptors. Annu Rev Plant Physiol 32:569–596CrossRefGoogle Scholar
  33. Seaman GVF, Heard DH (1960) The surface of washed human erythrocytes as a polyanion. J Gen Physiol 44:251–268PubMedCrossRefGoogle Scholar
  34. Senn A, Pilet PE (1980) Isolation and some physiological properties of maize root protoplasts. Z Pflanzenphysiol 100:299–310Google Scholar
  35. Senn A, Pilet PE (1981) Electrophoretic mobility, zeta potential and surface charges of maize root protoplasts. Z Pflanzenphysiol 102:19–32Google Scholar
  36. Senn A, Pilet PE (1982) Indoleacetic acid and abscisic acid effects on the electrokinetic properties of maize root protoplasts. In: Marmé D, Marrè E, Hertel R (eds) Plasmalemma and Tonoplast: their functions in the plant cell. Elsevier Biomedical, Amsterdam, pp 69–76Google Scholar
  37. Shaw DJ (1969) (ed) Electrophoresis. Academic, LondonGoogle Scholar
  38. Tenforde MB (1970) Microelectrophoretic studies on the surface chemistry of erythrocytes. Adv Biol Phys 13:34–105Google Scholar
  39. Thimann KV (1977) Hormone action in the whole life of plants. The Univ Massachussetts Press, Amherst:93–130Google Scholar
  40. Weiss L (1969) The cell periphery. Int Rev Cytol 26:63–105PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1985

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  • P. E. Pilet

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