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Some Physiological Properties of Protoplasts from Gravireacting Maize Roots

  • P. E. Pilet
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Protoplasts, with their exposed plasmalemma, have been used in studies of membrane integrity (Fowke et al. 1983). It has been clearly shown that they respond to auxin (such as indole-3-acetic acid: IAA) by increasing their rate of vacuolation until they ultimately burst (>Cocking 1962, Pilet 1971b, 1981). Such plasmalemma disruption, induced by IAA, may depend on the endogenous auxin level of the cells from which protoplasts have been obtained. If onion roots were treated with IAA before the preparation of protoplasts which were then incubated in medium containing IAA, the protoplasts burst at a higher IAA concentration (Pilet 1971b, 1981). Similarly, when using mesophyll protoplasts obtained from two species of tobacco and two of their tumor-prone interspecific hybrids (having an auxin level about 10 to 30 times higher than the two initial species) the bursting response of protoplasts showed that protoplasts from the tumorous hybrid tolerated auxin levels up to 30 times higher than did protoplasts from parent plants (Bayer 1973). It seems clear that protoplasts burst only after exogenous IAA reaches a level comparable with that of endogenous IAA present in the cells from which they were prepared (Pilet 1972, Hall and Cocking 1974, Pilet 1981).

Keywords

Zeta Potential Maize Root Mesophyll Protoplast Auxin Level Negative Zeta Potential 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer-Verlag Berlin Heidelberg 1985

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  • P. E. Pilet

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