Advertisement

Primary Structure and Processing of gag and env Gene Products of Human T-Cell Leukemia Viruses HTLV-ICR and HTLV-IATK

  • Stephen Oroszlan
  • Terry D. Copeland
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 115)

Abstract

Many of the immunological and biochemical properties of HTLV internal and envelope proteins are discussed in other chapters of this volume. Here we summarize the results of our published and ongoing studies on the primary structure of human T-0cell leukemia virus (HTLV) gag and env gene products. In addition to the previously known structural proteins p19, p24, p15, and gp46, the use of antibodies to synthetic peptides enabled us to characterize partially the transmembrane protein p21env and a low molecular weight glycoprotein gp16, which have not been identified previously.

Keywords

Bovine Leukemia Virus Complete Amino Acid Sequence Nucleic Acid Binding Protein Complete Primary Structure Primary Structure Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barbacid M, Stephenson JR, Aaronson SA (1976) gag gene of mammalian type C RNA tumor viruses. Nature 262:554–559PubMedCrossRefGoogle Scholar
  2. Copeland TD, Morgan MA, Oroszlan S (1983a) Complete amino acid sequence of the nucleic acid binding protein of bovine leukemia virus. FEBS Lett 156:37–40CrossRefGoogle Scholar
  3. Copeland TD, Oroszlan S, Kalyanaraman VS, Sarngadharan MG, Gallo RC (1983b) Complete amino acid sequence of human T-cell leukemia virus structural protein p15. FEBBS Lett 162:390–395Google Scholar
  4. Copeland TD, Morgan MA, Oroszlan S (1984) Complete amino acid sequence of the basic nucleic acid binding protein of feline leukemia virus. Virology 133: 137–145PubMedCrossRefGoogle Scholar
  5. Dittmar KJ, Moelling K (1978) Biochemical properties of pl5-associated protease in an avian RNA tumor virus. J Virol 28:106-118Google Scholar
  6. Edman P, Begg G (1967) A protein sequenator. Eur J Biochem 1: 80–91Google Scholar
  7. Essex M, McLane MF, Lee TH, Falk L, Howe CWS, Mullins JI, Cabradilla C, Francis DP (1983) Antibodies to cell membrane antigens associated with human T-cell leukemia virus in patients with AIDS. Science 220: 859–862PubMedCrossRefGoogle Scholar
  8. Gallo RC, Wong-Staal F (1982) Review: Retroviruses as etiologic agents of some animal and human leukemias and lymphomas and as tools for elucidating the molecular mechanims of leukemogenesis. Blood 60: 545–557PubMedGoogle Scholar
  9. Hattori S, Imagawa K, Shimizu F, Hashimura E, Seiki M, Yoshida M (1983) Identification of envelope glycoprotein encoded by env gene of human T-cell leukemia virus. Gan 74: 790–793PubMedGoogle Scholar
  10. Haynes BF, Robert-Guroff M, Metzgar RS, Franchini G, Kalyanaraman VS, Palker TJ, Gallo RC (1983) Monoclonal antibody against human T-cell leukemia virus pl9 defines a human thymic epithelial antigen acquired during ontogeny. J Exp Med 157: 907–920PubMedCrossRefGoogle Scholar
  11. Henderson LE, Copeland TD, Sowder RC, Smythers GW, Oroszlan S (1981) Primary strueture of the low-molecular-weight nucleic acid binding proteins of murine leukemia viruses. J Biol Chem 256: 8400–8406PubMedGoogle Scholar
  12. Henderson LE, Krutzsch HC, Oroszlan S (1983) Myristyl amino terminal aeylation of murine retroviral proteins: a new posttranslational protein modiflcation. Proc Natl Acad Sei USA 80: 339–343CrossRefGoogle Scholar
  13. Hinuma Y, Nagata K, Hanoka M, Nakai M, Matsumoto T, Kinoshita KI, Shirakawa S, Miyoshi I (1981) Adult T-cell leukemia antigen in ATL cell line and detection of antibodies to the antigen in human sera. Proc Natl Acad Sei USA 79: 4780–4782Google Scholar
  14. Hunter E, Bennett JC, Bhown A, Pepinsky RB, Vogt VM (1983) Amino-terminal acid sequence of plO, the fifth major gag polypeptide of avian sarcoma and leukemia viruses. J Virol 45: 885–888PubMedGoogle Scholar
  15. Kalyanaraman VS, Sarngadharan MG, Poiesz BJ, Ruscetto FW, Gallo RC (1981) Immunological properties of a type C retrovirus isolated from cultured T-lymphoma cells and comparison to other mammalian retroviruses. J Virol 38: 906–913PubMedGoogle Scholar
  16. Kalyanaraman VS, Morar MJ, Sarngadharan MG, Gallo RC (1984) Immunological characterization of the low molecular weight gag gene proteins pl9 and pl5 of human T-cell leukemia-lymphoma virus ( HTLV), and demonstration of human natural antibodies to them. Virology 132: 61–70Google Scholar
  17. Oroszlan S, Gilden RV (1980) Primary strueture analysis of retrovirus proteins. In: Stephenson JR (ed) Molecular biology of RNA tumor viruses. Academic, New York, pp 299–314Google Scholar
  18. Oroszlan S, Henderson LE, Stephenson JR, Copeland TD, Long CW, Ihle JN, Gilden RV (1978) Human T-Cell Leukemia Viruses HTLV-ICR and HTLV-Iatk 233 Amino- and carboxyl-terminal amino acid sequences of proteins coded by gag gene of murine leukemia virus. Proc Natl Acad Sei USA 75: 1404–1408CrossRefGoogle Scholar
  19. Oroszlan S, Copeland TD, Henderson LE, Stephenson JR, Gilden RV (1979) Amino-terminal sequence of bovine leukemia virus major internal protein: Homology with mammalian type C virus p30s. Proc Natl Acad Sei USA 76: 2996–3000CrossRefGoogle Scholar
  20. Oroszlan S, Sarngadharan MG, Copeland TD, Kalyanaraman VS, Gilden RV, Gallo RC (1982) Primary structure analysis of the major internal protein p24 of human type C T-cell leukemia virus. Proc Natl Acad Sei USA 79: 1291–1294CrossRefGoogle Scholar
  21. Reitz MS, Poiesz BJ, Ruscetti FW, Gallo RC (1981) Characterization and distribution of nucleic acid sequences of a novel type C retrovirus isolated from neoplastic human T lymphocytes. Proc Natl Acad Sei USA 78: 1887–1891CrossRefGoogle Scholar
  22. Robert-Guroff M, Ruscetti FW, Posner LE, Poiesz BJ, Gallo RC (1981) Detection of the human T cell lymphoma virus pl9 in cells of some patients with cutaneous T cell lymphoma and leukemia using a monoclonal antibody. J Exp Med 154: 1957–1964PubMedCrossRefGoogle Scholar
  23. Schneider J, Yamamoto N, Hinuma Y, Hunsmann G (1984) Sera from adult T-cell leukemia patients react with envelope and core Polypeptides of adult T-cell leukemia virus. Virology 132: 1–11PubMedCrossRefGoogle Scholar
  24. Schultz AM, Oroszlan S (1983) In vivo modification of retro viral gag gene encoded polyproteins by myristie acid. J Virol 46: 355–361PubMedGoogle Scholar
  25. Schultz AM, Henderson LE, Oroszlan S (1983) Myristylation of gag polyproteins of type B, C, and D retroviruses and also of retroviral transforming proteins. In: Rieh MA (ed) Leukemia reviews international, vol 1. Mareell Dekker, New York, p 304Google Scholar
  26. Schultz AM, Copeland TD, Oroszlan S (1984) The envelope proteins of bovine leukemia virus: Puriflcation and sequence analysis. Virology 135: 417–427Google Scholar
  27. Schüpbach J, Sarngadharan MG, Gallo RC (1984) Antigens on HTLV-infected cells recognized by leukemia and AIDS sera are related to HTLV viral glycoprotein. ScienceGoogle Scholar
  28. Schwartz DE, Tizard R, Gilbert W (1983) Nucleotide sequence of Rous sarcoma virus. Cell 32: 853–869PubMedCrossRefGoogle Scholar
  29. Seiki M, Hattori S, Hirayama Y, Yoshida M (1983) Human adult T-cell leukemia virus: Complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc Natl Acad Sei USA 80: 3618–3622CrossRefGoogle Scholar
  30. Tamura TA (1983) Provirus of M7 baboon endogenous virus: Nucleotide sequence of the gag-pol region. J Virol 47: 137–145PubMedGoogle Scholar
  31. Watanabe T, Seiki M, Yoshida M (1983) HTLV and ATLV. Science 222:1178PubMedCrossRefGoogle Scholar
  32. Yoshida M, Miyoshi I, Hinuma Y (1982) Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implications in the disease. Proc Natl Acad Sei USA 79: 2031–2035CrossRefGoogle Scholar
  33. Yoshinaka Y, Katoh I, Copeland TD, Oroszlan S (to be published) Puriflcation, biochemical and structural characterization of Mu-MuLV protease encoded by the gag-pol gene. Proc Natl Acad Sei USAGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1985

Authors and Affiliations

  • Stephen Oroszlan
    • 1
  • Terry D. Copeland
    • 1
  1. 1.Laboratory of Molecular Virology and Carcinogenesis, LBI-Basic Research ProgramNCI-Frederick Cancer Research FacilityFrederickUSA

Personalised recommendations