Skip to main content
SpringerLink
Log in

Preparation of Plant Mitochondria, Criteria for Assessement of Mitochondrial Integrity and Purity, Survival in Vitro

  • Chapter
Higher Plant Cell Respiration

Part of the book series: Encyclopedia of Plant Physiology ((PLANT,volume 18))

Abstract

Plant mitochondria are small organelles of the cytoplasmic compartment of the cell, involved in respiration and providing energy (ATP) and metabolic precursors for the rest of the cell. The mitochondria consist of a folded inner membrane involved in different processes (electron transport, metabolite transports via selective carriers, ATP synthesis, etc.) surrounding a matrix space containing all the tricarboxylic acid (TCA) cycle enzymes as well as a genetic system (DNA, RNA and ribosomes) for protein synthesis. The inner membrane is protected from the cytosol by an outer limiting membrane, the role of which remains uncertain. However the presence of b-type cytochrome (DOUCE et al. 1973), porine (Zalman et al. 1980) and hexokinase (Tanner et al. 1983) demonstrates that the external membrane must perform several specific functions as well as a mechanical role. In addition, little is known about the presence of soluble enzymes in the intermembrane space and their possible functions.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Anderson JW, Rowan KS (1966) Extraction of soluble leaf enzymes with thiols and other reducing agents. Phytochemistry 6:1047–1056.

    Article  Google Scholar 

  • Arron GP, Edwards GE (1979) Oxidation of reduced nicotinamide adenine dinucleotide phosphate by plant mitochondria. Can J Biochem 57:1392–1399.

    Article  PubMed  CAS  Google Scholar 

  • Arron GP, Spalding MH, Edwards GE (1979) Isolation and oxidative properties of intact mitochondria from the leaves of Sedum praealtum. Plant Physiol 64:182–186.

    Article  PubMed  CAS  Google Scholar 

  • Baker JE, Elfvin LG, Biale JB, Honda SI (1968) Studies of ultrastructure and purification of isolated plant mitochondria. Plant Physiol 43:2001–2022.

    Article  PubMed  CAS  Google Scholar 

  • Beevers H (1982) Glyoxysomes in higher plants. Ann NY Acad Sci 386:243–253.

    Article  CAS  Google Scholar 

  • Bergman A, Gardeström P, Ericson I (1980) Method to obtain a chlorophyll-free preparation of intact mitochondria from spinach leaves. Plant Physiol 66:442–445.

    Article  PubMed  CAS  Google Scholar 

  • Bligny R, Douce R (1978) Calcium-dependent lipolytic acyl-hydrolase activity in purified plant mitochondria. Biochim Biophys Acta 529:419–428.

    PubMed  CAS  Google Scholar 

  • Bonner WD Jr (1967) A general method for the preparation of plant mitochondria. In: Estabrook RW, Pullman ME (eds) Methods in enzymology, vol X. Academic Press, London New York. pp 126–133.

    Google Scholar 

  • Day DA, Wiskich JT (1980) Glycine transport by pea leaf mitochondria. FEBS Lett 112:191–194.

    Article  CAS  Google Scholar 

  • Day DA, Neuburger M, Douce R (1984) Activation of NAD-linked malic enzyme in intact plant mitochondria by exogenous coenzyme A. Arch Biochem Biophys 231:233–242.

    Article  PubMed  CAS  Google Scholar 

  • Douce R (1974) Site of galactolipid synthesis in spinach chloroplasts. Science 183:852–853.

    Article  PubMed  CAS  Google Scholar 

  • Douce R, Joyard J (1979) Structure and function of the plastid envelope. In: Woolhouse HH (ed) Advances in botanical research, vol VII. Academic Press, London New York. pp 1–116.

    Google Scholar 

  • Douce R, Lance C (1972) Alteration des activités oxydatives et phosphorylantes des mitochondries de chou-fleur sous l’action de phospholipases et du vieillissement. Physiol Vég 10:181–198.

    CAS  Google Scholar 

  • Douce R, Christensen EL, Bonner WD (1972) Preparation of intact plant mitochondria. Biochim Biophys Acta 275: 148–160.

    Article  PubMed  CAS  Google Scholar 

  • Douce R, Manella CA, Bonner WD (1973) The external NADH dehydrogenases of intact plant mitochondria. Biochim Biophys Acta 292:105–116.

    Article  PubMed  CAS  Google Scholar 

  • Douce R, Moore AL, Neuburger M (1977) Isolation and oxidative properties of intact mitochondria isolated from spinach leaves. Plant Physiol 60:625–628.

    Article  PubMed  CAS  Google Scholar 

  • Dupont J, Rustin P, Lance C (1982) Interaction between mitochondrial cytochromes and linoleic acid hydroperoxide. Possible confusion with lipoxygenase and alternative pathway. Plant Physiol 69:1308–1314.

    Article  PubMed  CAS  Google Scholar 

  • Fleischer S, Kervina M (1974) Long-term preservation of liver for subcellular fractionation. In: Fleischer S, Packer L (eds) Methods in enzymology, vol 31/A. Academic Press, London New York. pp 3–41.

    Google Scholar 

  • Galliard T (1980) Degradation of acyl lipids: hydrolytic and oxidative enzymes. In: Stumpf PK, Conn EE (eds) The biochemistry of plants, vol IV. Academic Press, London New York. pp 85–116.

    Google Scholar 

  • Galliard T, Chan HW-S (1980) Lipoxygenases. In: Stumpf PK, Conn EE (eds) The biochemistry of plants, vol IV. Academic Press, London New York. pp 131–161.

    Google Scholar 

  • Goldstein AH, Anderson JO, McDaniel RG (1981) Cyanide-insensitive and cyanide-sensitive O2 uptake in wheat. Plant Physiol 67:594–596.

    Article  PubMed  CAS  Google Scholar 

  • Hanson JB, Day DA (1980) Plant mitochondria. In: Stumpf PK, Conn EE (eds) The biochemistry of plants, vol I. Academic Press, London New York. pp 315–358.

    Google Scholar 

  • Haurowitz F, Schwerin P, Yenson MM (1941) Destruction of hemin and hemoglobin by the action of unsaturated fatty acids and oxygen. J Biol Chem 140:353–359.

    CAS  Google Scholar 

  • Hulme AC, Jones JD, Wooltorton LSC (1964) Mitochondrial preparations from the fruit of the apple. Preparation and general activity. Phytochemistry 3:173–188.

    Article  CAS  Google Scholar 

  • Jackson C, Dench JE, Hall DO, Moore AL (1979) Separation of mitochondria from contaminating subcellular structures utilizing silica sol gradient centrifugation. Plant Physiol 64:150–153.

    Article  PubMed  CAS  Google Scholar 

  • Lance C, Hobson GE, Young RE, Biale JB (1967) Metabolic processes in cytoplasmic particles of the avocado fruit. IX the oxidation of pyruvate and malate during the climacteric cyle. Plant Physiol 42:471–478.

    Article  PubMed  CAS  Google Scholar 

  • Loomis WD (1974) Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. In: Fleischer S, Packer L (eds) Methods in enzymology, vol 31/A. Academic Press, London New York. pp 528–544.

    Google Scholar 

  • Loomis WD, Battaile J (1966) Plant Phenolic compounds and the isolation of plant enzymes. Phytochemistry 5:423–438.

    Article  CAS  Google Scholar 

  • Matile Ph (1976) Vacuoles. In: Bonner J, Varner JE (eds) Plant biochemistry, 3rd edn. Academic Press, London New York. pp 189–224.

    Google Scholar 

  • McCarty RE, Douce R, Benson AA (1973) The acyl lipids of highly purified plant mitochondria. Biochim Biophys Acta 316:266–270.

    PubMed  CAS  Google Scholar 

  • Mettler IJ, Beevers H (1980) Oxidation of NADH in glyoxysomes by a malate-aspartate shuttle. Plant Physiol 66:555–560.

    Article  PubMed  CAS  Google Scholar 

  • Moreau F, Romani R (1982) Preparation of Avocado mitochondria using self generated Percoll density gradients and changes in buoyant density during ripening. Plant Physiol 70:1380–1384.

    Article  PubMed  CAS  Google Scholar 

  • Nash D, Wiskich JT (1983) Properties of substantially chlorophyll-free pea leaf mitochondria prepared by sucrose density gradient separation. Plant Physiol 71:627–634.

    Article  PubMed  CAS  Google Scholar 

  • Neuburger M, Douce R (1977) Oxydation du malate, du NADH et de la glycine par les mitochondries des plantes en C3 et C4. CR Acad Sci 285:881–884.

    CAS  Google Scholar 

  • Neuburger M, Douce R (1983) Slow passive diffusion of NAD+ between intact isolated plant mitochondria and suspending medium. Biochem J 216:443–450.

    PubMed  CAS  Google Scholar 

  • Neuburger M, Journet EP, Bligny R, Carde JP, Douce R (1982) Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll. Arch Biochem Biophys 217:312–323.

    Article  PubMed  CAS  Google Scholar 

  • Nishimura M, Beevers H (1978) Hydrolases in vacuoles from castor bean endosperm. Plant Physiol 62:44–48.

    Article  PubMed  CAS  Google Scholar 

  • Nishimura M, Douce R, Akazawa T (1982) Isolation and characterization of metabolically competent mitochondria from spinach leaf protoplasts. Plant Physiol 69:916–920.

    Article  PubMed  CAS  Google Scholar 

  • Palmer JM (1976) Electron transport in plant mitochondria. Annu Rev Plant Physiol 27:133–157.

    Article  CAS  Google Scholar 

  • Palmer JM, Kirk BI (1974) The influence of osmolarity on the reduction of exogenous cytochrome c and permeability of the inner membrane of Jerusalem artichoke mitochondria. Biochem J 140:79–86.

    PubMed  CAS  Google Scholar 

  • Quail PH (1979) Plant cell fractionation. Annu Rev Plant Physiol 30:425–484.

    Article  CAS  Google Scholar 

  • Ravanel P, Tissut M, Douce R (1981) Effects of flavone on the oxidative properties of intact plant mitochondria. Phytochemistry 20:2101–2103.

    Article  CAS  Google Scholar 

  • Ravanel P, Tissut M, Douce R (1982) Uncoupling activities of chalcones and dihydrochal-cones on isolated mitochondria from potato tubers and mung bean hypocotyls. Phytochemistry 21:2845–2850.

    Article  CAS  Google Scholar 

  • Schwitzguebel JP, Moller IM, Palmer JM (1981) Change in density of mitochondria and glyoxysomes from Neurospora crassa: a re-evaluation utilizing silica sol gradient centrifugation. J Gen Microbiol 126:289–295.

    CAS  Google Scholar 

  • Siedow JN, Girvin ME (1980) Alternative respiratory pathway. Plant Physiol 65:669–674.

    Article  PubMed  CAS  Google Scholar 

  • Sitaramam V, Sambasiva R (1984) The intermembranous space and the structure of mitochondrial membrane in oxidative phosphorylation. Trends Biochem Sci 9:222–223.

    Article  Google Scholar 

  • Tanner GJ, Copeland L, Turner JF (1983) Subcellular localization of hexose kinases in pea stems: mitochondrial hexokinase. Plant Physiol 72:659–663.

    Article  PubMed  CAS  Google Scholar 

  • Tolbert NE (1982) Leaf peroxisomes. Ann NY Acad Sci 386:254–268.

    Article  CAS  Google Scholar 

  • Weinbach EC, Garbus J (1966) Restoration by albumin of oxidative phosphorylation and related reactions. J Biol Chem 241:169–175.

    PubMed  CAS  Google Scholar 

  • Wiskich JT, Bonner WD (1963) Preparation and properties of sweet potato mitochondria. Plant Physiol 38:594–603.

    Article  PubMed  CAS  Google Scholar 

  • Wojtczak L, Zaluska H (1969) On the permeability of the outer mitochondrial membrane to cytochrome c.I. Studies of whole mitochondria. Biochim Biophys Acta 193:64–72.

    Article  PubMed  CAS  Google Scholar 

  • Zalman LS, Nikaido H, Kagawa Y (1980) Mitochondrial outer membrane contains a protein producing nonspecific diffusion channels. J Biol Chem 255:1771–1774.

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. M. Neuburger
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Centre d’ Etudes Nucléaires et Université Scientifique et Médicale de Grenoble, F-38041, Grenoble Cedex, France

    Roland Douce

  2. Département de Recherche Fondamentale Laboratoire de Physiologie Cellulaire Végétale, UN CNRS N° 576 85X, F-38041, Grenoble Cedex, France

    Roland Douce

  3. Botany Department, The Australian National University, Box 475 P.O., Canberra City, ACT, 2601, Australia

    David A. Day

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Neuburger, M. (1985). Preparation of Plant Mitochondria, Criteria for Assessement of Mitochondrial Integrity and Purity, Survival in Vitro. In: Douce, R., Day, D.A. (eds) Higher Plant Cell Respiration. Encyclopedia of Plant Physiology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70101-6_2

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-70101-6_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70103-0

  • Online ISBN: 978-3-642-70101-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation