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Gentransfer in eukaryotische Zellen

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Book cover Molekular- und Zellbiologie

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Zusammenfassung

Dieser Artikel gibt einen kurzen Abriß über die Methoden des Gentransfers in kultivierte Säugerzellen. Soweit bekannt, soll über den Verbleib des genetischen Materials nach dem Transfer in die Zellen berichtet werden. Wegen der Vielfalt der Beispiele wird die Expression von transferierten Genen verhältnismäßig kurz beschrieben. Die Ausführungen sollen eine Anregung dafür geben, welche Fragestellungen mit den angeführten Methoden beantwortet werden können. Eine Beschränkung auf den Gentransfer mit isolierter DNA ist bewußt vorgenommen worden, obgleich es für viele Fragestellungen methodische Überlappungen mit einer Reihe anderer Methoden wie z. B. der Zellfusion und dem Transfer von Chromosomen oder DNA-Protein-Komplexen gibt.

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Authors and Affiliations

  1. Gesellschaft für Biotechnologische Forschung, D-3300, Braunschweig, Deutschland

    H. Hauser

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  1. H. Hauser
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  1. Institut für Humangenetik, Universität des Saarlandes, Universitätsklinik Gebäude 68, 6650, Homburg, Deutschland

    Nikolaus Blin

  2. Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 6900, Heidelberg, Deutschland

    Michael F. Trendelenburg

  3. Institut für Genetik, Ruhr-Universität, Postfach 10 21 48, 4630, Bochum 1, Deutschland

    Erwin R. Schmidt

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Hauser, H. (1985). Gentransfer in eukaryotische Zellen. In: Blin, N., Trendelenburg, M.F., Schmidt, E.R. (eds) Molekular- und Zellbiologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70100-9_13

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