Abstract
In-vitro cloning assays in semi-solid agar have been demonstrated to be very useful in the study of growth and differentiation of human normal and malignant hematopoietic cells (Metcalf 1977; Hamburger 1977). Several sophisticated preservation procedures of these cultures have been described in an attempt to simplify morphological and cytochemical characterisation of single cell clusters (Kubota et al. 1980; Konwalinka et al. 1980; Fauser and Messmer 1979; Salmon and Buick 1979; Jakobs et al. 1979). Until the present time none of the reported techniques has been satisfactory for the in-situ identification of the various proliferating cell types.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bamstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other cell surface antigens — new tools for genetic analysis. Cell 14: 9–20
Fauser AA, Messner HA (1979) Identification of megakaryocytes, macrophages, and eosinophiles in colonies of human bone marrow containing neutrophilic granulocytes and erythroblasts. Blood 53: 1023–1027
Hamburger AW, Salmon SE (1977a) Primary bioassay of human tumor stem cells. Science 197: 461–463
Hamburger AW, Salmon SE (1977b) Primary bioassay of human myeloma stem cells. J Clin Invest 60: 846–854
Hamburger AW, Kim MB, Salmon SE (1977 c) Characterisation of the myeloma colony-forming cell. Transplantation Immunol: 155-162
Jakobs B, Dubovsky D, Smith S, Randall G, Bracher M (1978) Bone marrow culture in vitro. A technique for analysis and permanent recording of cellular composition. Exp Haemat 7: 177–182
Kamoun M, Martin PJ, Hansen JA, Brown MA, Siadak AW, Nowinski RC (1981) Identification of a human T lymphocyte surface protein associated with the E-rosette receptor. J Exp Med 153: 207–212
Konwalinka G, Glaser P, Odavic R, Bogusch E, Schmalzl F, Braunsteiner H (1980) A new approach to the morphological and cytochemical evaluation of human bone marrow CFUc in agar gel. Exp Haemat 8: 434–440
Kubota K, Mizoguchi H, Micera Y, Suda T, Takaku F (1980) A new technique for the cytochemical examination of human haematopoietic cells grown in agar gel. Exp Haemat 8: 339–344
Metcalf D (1977) Hemopoietic colonies. Springer, Berlin Heidelberg New York
Salmon SE, Buick RN (1979) Preparation of permanent slides of intact colony cultures of hematopoietic and tumor stem cells. Cancer Res 39: 1133–1136
Stashenko P, Nadler LM, Hardy R, Schlossman SF (1980) Characterisation of a human B lymphocyte specific antigen. J Immunol 125: 1678–1685
Stein H, Tolksdorf G, Lennert K, Rodt H, Gerdes J (1981) Immunohistologic analysis of the organisation of normal lymphoid tissue and Non-Hodgkin’s lymphomas. J Histochem Cytochem 28: 746–760
Ziegler A, Milstein C (1979) A small polypeptide different from β 2-microglobulin associated with a human cell surface antigen. Nature 279: 243–244
Ziegler A, Heining J, Müller C, Götze H, Thinnes FP, Uchanska-Ziegler B, Wernet P (submitted) Analysis by sequential immunoprecipitations of the monoclonal antibodies TÜ22, 34, 35, 36, 37, 39, 43, 58, and YD 1/63. HLK directed against human HLA-class II antigens. Immunobiology
Rights and permissions
Copyright information
© 1985 Springer-Verlag Berlin, Heidelberg
About this paper
Cite this paper
Müller, C., Busch, F.W. (1985). Immunhistological Analysis of Myeloma Colony-Forming Units by a new Preservation Technique. In: Aktuelle Aspekte der Tumor-Immunologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70097-2_6
Download citation
DOI: https://doi.org/10.1007/978-3-642-70097-2_6
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-13924-9
Online ISBN: 978-3-642-70097-2
eBook Packages: Springer Book Archive