Abstract
Sprague-Dawley rats were kept under a normal 12-h day-night cycle, fed standard rat diet, and given access to water ad libitum. Male and female rats were mated in a ratio of 1:4. The day at which the vaginal smear was characteristic for estrus and also contained sperm was counted as embryonic day 1 (E1). Thereafter, the pregnant females were placed in separate cages. The terms “embryonic” and “fetal” will be used for the periods E 1–E 16 and E 17–E 22 (birth) respectively. A total of 174 animals were delivered by cesarean section between E12 and E22. At each embryonic day, at least six embryos from two or more pregnant rats were used; four times as many animals were taken at E14, E 15, E18, and E21.
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© 1985 Springer-Verlag Berlin Heidelberg
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Wolff, J.R., Rickmann, M. (1985). Material and Methods. In: Prenatal Gliogenesis in the Neopallium of the Rat. Advances in Anatomy Embryology and Cell Biology, vol 93. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70081-1_2
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DOI: https://doi.org/10.1007/978-3-642-70081-1_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-13849-5
Online ISBN: 978-3-642-70081-1
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