Summary
Enzyme immunoassay (ELISA) has deservedly become widely established in developed countries as a rapid, sensitive and specific tool for detecting and quantitating a wide range of antigens and antibodies. Its merits include the relatively low cost of consumables, reagent stability, reproducibility and case of procedure. Since ELISA can readily be employed in the Third World, its many potential applications in such regions will briefly be detailed. The advantages of ELISA over other assays will also be described and mention will be made of its application to rabies and to the technical problems that are sometimes encountered.
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References
Yolken RH (1982) Enzyme immunoassays for the rapid diagnosis of viral infections In ‘Medical Virology’ eds L. de la Maza and E.M. Peterson. pp 55–89. Elsevier Biomedical, New York.
Sikes RK, Cleary WF, Koprowski H, Wiktor TJ, Kaplan MM (1971) Effective protection of monkeys against death from street virus by post-exposure administration of tissue-culture rabies vaccine. Bull. WHO. 45 1–11.
Turner GS (1978) Immunoglobulin IgG and IgM antibody responses to rabies vaccine. J. Gen Virol. 40:595–604.
Nicholson KG, Burney MI, Ali S, Perkins FT (1983) Stability of human diploid cell strain rabies vaccine at high ambient temperatures. Lancet i 916–918.
Nicholson KG, Prestage H (1982) Enzyme-linked immunosorbent assay: a rapid reproducible test for the measurement of rabies antibody. J. Med Virol. 9 43–49.
Atanasiu P, Savy V, Perrin P (1977) Epreuve immunoenzymatique pour la detection rapide des anticorps antirabiques. Ann Microbiol (Inst Past). 128A:489–498.
Thraenhart O, Kuwert E (1979) Rabies specific IgM and IgG-antibody response in persons immunized with HDCS vaccine according to the Essen post-exposure vaccination schedule. Zbl. Bakt. Hyg. 1. Abt. Orig. A 245, 429–441.
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© 1985 Springer-Verlag Berlin, Heidelberg
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Nicholson, K.G., Warrell, M.J., Xueref, C., Lee, S. (1985). Enzyme Immunoassay in the Tropics. In: Kuwert, E., Mérieux, C., Koprowski, H., Bögel, K. (eds) Rabies in the Tropics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70060-6_46
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DOI: https://doi.org/10.1007/978-3-642-70060-6_46
Publisher Name: Springer, Berlin, Heidelberg
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