Summary
Continuing research in many parts of the world resulted in steadily increasing knowledge about the rabies virion, its behaviour in tissue culture, and the use of tissue culture procedures for vaccine production. Tissue culture vaccines for veterinary use appeared on the market towards the end of the sixties, and we survey about 15 years of experience with a tissue culture vaccine for veterinary use based on the Flury LEP-C23 strain as the vaccine strain, and primary chick embryo cells as the cell substrate. First the virus was inactivated by heat, later on by betapropio-lactone. Further development of this vaccine led to an antigen which was concentrated and purified by continuous density gradient centrifugation a purification method which so far has only been applied for vaccines for human use. By this method, pure and highly concentrated inactivated antigen concentrates are achieved which are evaluated by the modified antibody binding test and diluted to give a potent vaccine. This procedure has proved to be very valuable in the manufacture of vaccine batches with standardized potency in monovalent and combined vaccines. Some results from laboratory tests and from the field with these vaccines are reported.
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References
Hilfenhaus J, Kohler R, Barth R, Majer M, Mauler R (1976) Large-scale purification of animal viruses in the RK-model zonal ultracentrifuge. J Biol Stand 4:263–271
Barth R, Groß-Albenhausen F, Jaeger O, Milcke L (1981) The antibody binding test, a useful method for quantitative determination of inactivated rabies virus antigen. J Biol Stand 9:81–89
Barth R, Gruschkau H, Jaeger O, Milcke L (1982) Purification, concentration and evaluation of rabies virus antigen. Comp Immun Microbiol Infect Dis 5:211–216
Barth R, Jaeger O (1968) Untersuchungen mit der Lebendvaccine VIRULIN ad us vet aus dem attenuierten Tollwutvirusstamm FLURY-LEP. Tierärztl Wschr, Berlin München, 81:30–35
Jaeger O, Barth R (1972) Untersuchungen mit inaktivierten Gewebekulturimpfstoffen am Tier. IV. Teil: Immunitätsdauer am Hund. Tierärztl Wschr, Berlin München, 85:382–384
Barth R, Jaeger O (1977) Zur Prüfung der Immunitätsdauer von Tollwut-Kombinations-vaccinen am Hund. Die Blauen Hefte für den Tierarzt 57:337–346
Kihm U, Lazarowicz M, Bommeli W, Zutter R (1982) Potency of two rabies vaccines in cats as determined by antibody assay and virulent virus challenge. Comp Immun Microbiol infect Dis 5:227–232
Wachendörfer G, Förster U, Krekel H (1974) Production of serum neutralizing antibody in sheep after a single injection of an inactivated rabies vaccine of tissue culture origin. Symp Ser Immunbiol Stand 21:277–282
Barth R, Becker W, Jaeger O (1971) Untersuchungen mit inaktivierten Tollwut-Gewebekulturimpfstoffen am Tier. II. Teil: Immunisierungsversuche an Rindern. Tierärztl Wschr, Berlin München, 84:167–172
Jaeger O, Barth R, Becker W (1972) Untersuchungen mit inaktivierten Tollwut-Gewebekulturimpfstoffen am Tier. III. Teil: Immunitätsdauer beim Rind. Tierärztl Wschr, Berlin München, 85:167–169
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© 1985 Springer-Verlag Berlin, Heidelberg
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Barth, R., Gruschkau, H., Jaeger, O. (1985). Chick-Embryo-Cell Inactivated Rabies Vaccine for Veterinary Use. Laboratory and Field Experience. In: Kuwert, E., Mérieux, C., Koprowski, H., Bögel, K. (eds) Rabies in the Tropics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70060-6_32
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DOI: https://doi.org/10.1007/978-3-642-70060-6_32
Publisher Name: Springer, Berlin, Heidelberg
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