Gamma-Glutamyltransferase and Other Markers for Alcoholism

  • R. Teschke


Alcoholism is a wide spread disease, and the growing prevalence of alcohol-related disorders creates major medical and social problems throughout the developed world. Medical complications of alcoholism may include a variety of organs such as liver (Teschke 1981), pancreas (Goebell and Singer 1981), esophagus (Wienbeck and Berges 1981), gastrointestinal tract (Berges and Wienbeck 1981), heart (Kuhn 1981), bones (Lindsell et al. 1982), endocrine glands (Kley et al. 1981), brain (Feuerlein 1981), and peripheral nerves (Schrappe 1981), and recognizing these diseases as alcohol-related ones is usually not a problem.


Alcoholic Liver Disease Mean Corpuscular Volume Alcoholic Patient Chronic Alcohol Consumption Alcoholic Liver Cirrhosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bagrel A, d’Houtaud A, Gueguen R, Siest G (1979) Relations between reported alcohol consumption and certain biological variables in an “unselected” population. Clin Chem 25: 1242–1246PubMedGoogle Scholar
  2. Bagrel D, Ratanasavanh D, Siest G (1980) Influence of three inducers on rabbit gamma-glutamyltransferase. Pharmacol Res Commun 12: 557–565PubMedCrossRefGoogle Scholar
  3. Barnwell SG, Godfrey PP, Lowe PJ, Coleman R (1983) Biliary protein output by isolated perfused rat livers. Effects of bile salts. Biochem J 210: 549–557PubMedGoogle Scholar
  4. Barouki R, Chobert MN, Finidori J, Aggerbeck M, Nalpas B, Hanoune J (1983) Ethanol effects in a rat hepatome cell line: induction of y-glutamyltransferase. Hepatology 3: 323–329PubMedCrossRefGoogle Scholar
  5. Bartels H, Evert W, Hauck W, Petersen C, Putzki H, Schulze W (1975) Significance of increased serum gamma-glutamyltransferase activity during long-term anticonvulsive treatment, clinical and experimental studies. Neuropaediatrie 6: 77–89CrossRefGoogle Scholar
  6. Belfrage P, Berg B, Hägerstrand I, Nilsson-Ehle P, Tornqvist H, Wiebe T (1977) Alterations of lipid metabolism in healthy volunteers during long-term ethanol intake. Eur J Clin Invest 7: 127–131PubMedCrossRefGoogle Scholar
  7. Berges W, Wienbeck M (1981) Alkohol und Gastrointestinaltrakt. In: Teschke R, Lieber CS (eds) Alkohol und Organschaden. Epidemiologische, klinische, biochemische und therapeutische Aspekte. Witzstrock, Baden-Baden, pp 74–88Google Scholar
  8. Bernadt MW, Mumford J, Taylor C, Smith B, Murray RM (1982) Comparison of questionnaire and laboratory tests in the detection of excessive drinking and alcoholism. Lancet 1: 325–328PubMedCrossRefGoogle Scholar
  9. Billington D, Juszczak R, Rahman K (1983) Chenodeoxycholate feeding increases the output of plasma-membrane marker enzymes into rat bile. Biochem Soc Trans 11: 162Google Scholar
  10. Busachi C, Mebis J, Broeckaert L, Desmet V (1981) Histochemistry of y-glutamyl transpeptidase in human liver biopsies. Pathol Res Pract 172: 99–108PubMedGoogle Scholar
  11. Carney MWP, Sheffield B (1978) Serum folate and B12 and haematological status of in-patient alcoholics. Br J Addict 73: 3–7CrossRefGoogle Scholar
  12. Chalmers DM, Chanarin I, MacDermott S, Levi A J (1980) Sex-related differences in the haematological effects of excessive alcohol consumption. J Clin Pathol 33: 3–7PubMedCrossRefGoogle Scholar
  13. Chalmers DM, Rinsler MG, MacDermott S, Spicer CC, Levi AJ (1981) Biochemical and haematological indicators of excessive alcohol consumption. Gut 22: 992–996PubMedCrossRefGoogle Scholar
  14. Chick J, Kreitman N, Plant M (1981) Mean cell volume and gamma-glutamyl-transpeptidase as markers of drinking in working men. Lancet 1: 1249–1251PubMedCrossRefGoogle Scholar
  15. Clarenburg R (1983) Asialoglycoprotein receptor is uninvolved in clearing intact glycoproteins from rat blood. Am J Physiol 244: G247–G253PubMedGoogle Scholar
  16. Clark PMS, Kricka LJ, Zaman S (1982) Drivers, binge drinking, and gamma-glutamyltranspeptidase. Br Med J 285: 1656–1657CrossRefGoogle Scholar
  17. Cushman P, Jacobson G, Barboriak JJ, Anderson AJ (1984) Biochemical markers for alcoholism: sensitivity problems. Alcoholism: Clin Exp Res 8: 253–257CrossRefGoogle Scholar
  18. Debanne MT, Evans WH, Flint N, Regoeczi E (1982) Receptor-rich intracellular membrane vesicles transporting asialotransferrin and insulin in liver. Nature 298: 398–400PubMedCrossRefGoogle Scholar
  19. Dienstag JL, Carter EA, Wands JR, Isselbacher KJ, Fischer JE (1978) Plasma a-amino-n-butyric acid to leucine ratio. Nonspecificity as a marker for alcoholism. Gastroenterology 75: 561–565PubMedGoogle Scholar
  20. Doyle D, Le AV (1981) Regulation of the metabolism of plasma membrane proteins in hepatocytes. Hepatology 1: 184–186PubMedCrossRefGoogle Scholar
  21. Dunbar JA, Hagart J, Martin B, Ogston S, Devgun MS (1982) Drivers, binge drinking, and gamma-glutamyltranspeptidase. Br Med J 285: 1083CrossRefGoogle Scholar
  22. Ebihara Y, Ishii H, Takagi T, Miura S, Munakata Y, Takagi S, Arai M, Shigeta Y, Kono H, Tsuchiya M (1982) Role of intestinal gamma glutamyltranspeptidase (GGTP) activity in enhanced plasma GGTP level in alcoholics. Alcoholism 6: 294 AGoogle Scholar
  23. Eckardt MJ, Ryback RS, Rawlings RR, Graubard BI (1981) Biochemical diagnosis of alcoholism. A test of the discriminating capabilities of y-glutamyl transpeptidase and mean corpuscular volume. JAMA 246: 2707–2710PubMedCrossRefGoogle Scholar
  24. Eichner ER, Hillman RS (1971) The evolution of anemia in alcoholic patients. Am J Med 50: 218–232PubMedCrossRefGoogle Scholar
  25. Eisenberg K, Ortmans H (1973) Gamma-Glutamyl-Transpeptidase-Aktivität bei Lebererkrankungen. Med Klin 68: 798–804PubMedGoogle Scholar
  26. Ellingboe J, Mendelson JH, Varanelli CC, Neuberger O, Borysow M (1978) Plasma alpha amino-H-butyric acid: leucine ratio. Normal values in alcoholics. J Stud Alcohol 39: 1467–1476PubMedGoogle Scholar
  27. Evans WH (1981) Membrane traffic at the hepatocyte’s sinusoidal and canalicular surface domains. Hepatology 1: 452–457PubMedCrossRefGoogle Scholar
  28. Faller J, Fox IH (1982) Ethanol-induced hyperuricemia. Evidence for increased urate production by activation of adenine nucleotide turnover. N Engl J Med 307: 1598–1602PubMedCrossRefGoogle Scholar
  29. Feuerlein W (1981) Psychiatrische Krankheitsbilder des Alkoholismus. In: Teschke R, Lieber CS (eds) Alkohol und Organschäden. Epidemiologische, klinische, biochemische und therapeutische Aspekte. Witzstrock, Baden-Baden, pp 225–236Google Scholar
  30. Feuerlein W, Ringer C, Küfner H, Antons K (1977) Diagnose des Alkoholismus. Der Münchner Alkoholismustest ( MALT ). MMW 119: 1275–1282Google Scholar
  31. Freer DE, Statland BE (1977a) The effects of ethanol (0.75 g/kg body weight) on the activities of selected enzymes in sera of healthy young adults: 1. Intermediate-term effects. Clin Chem 23: 830–834Google Scholar
  32. Freer DE, Statland BE (1977b) Effects of ethanol (0.75 g/kg body weight) on the activities of selected enzymes in sera of healthy young adults: 2. Interindividual variations in response of y-glutamyltransferase to repeated ethanol challenges. Clin Chem 23: 2099–2102Google Scholar
  33. Gadeholt G, Aarbakke J, Dybing E, Sjöblom M, Mörland J (1980) Hepatic microsomal drug metabolism, glutamyl transferase activity and in vivo antipyrine half-life in rats chronically fed an ethanol diet, a control diet and a chow diet. J Pharmacol Exp Ther 213: 196–203PubMedGoogle Scholar
  34. Geuze HJ, Slot JW, Strous GJAM, Lodish HF, Schwartz AL (1982) Immunocytochemical localization of the receptor for asialoglycoprotein in rat liver cells. J Cell Biol 92: 865–870PubMedCrossRefGoogle Scholar
  35. Gill GV, Baylis PH, Flear CTG, Skillen AW, Diggle PH (1982) Acute biochemical responses to moderate beer drinking. Br Med J 285: 1770–1773CrossRefGoogle Scholar
  36. Goebell H, Singer MV (1981) Alkohol und Pankreas. In: Teschke R, Lieber CS (eds) Alkohol und Organschäden. Epidemiologische, klinische, biochemische und therapeutische Aspekte. Witzstrock, Baden-Baden, pp 111–123Google Scholar
  37. Heidemann E, Nerke O, Waller HD (1981) Alkoholtoxische Veränderungen der Hämatopoiese. Eine prospektive Studie bei chronischen Alkoholikern. Klin Wochenschr 59: 1303–1312PubMedCrossRefGoogle Scholar
  38. Herbert V, Zalusky R, Davidson CS (1964) Correlation of folate deficiency with alcoholism and associated macrocytosis, anemia, and liver disease. Ann Intern Med 58: 977–988Google Scholar
  39. Hoberman HD, Chiodo SM (1982) Elevation of the hemoglobin Al fraction in alcoholism. Alcoholism 6: 260–266PubMedGoogle Scholar
  40. Hultberg B, Isaksson A, Tiderström G (1980) Hexosaminidase, leucine aminopeptidase, cystidyl aminopeptidase, hepatic enzymes and bilirubin in serum of chronic alcoholics with acute ethanol intoxication. Clin Chim Acta 105: 317–323PubMedCrossRefGoogle Scholar
  41. Hurt RD, Morse RM, Swenson WM (1980) Diagnosis of alcoholism with a self-administered alcoholism screening test. Results with 1,002 consecutive patients receiving general examinations. Mayo Clin Proc 55: 365–370PubMedGoogle Scholar
  42. Huseby N-E (1977) Purification and some properties of y-glutamyltransferase from human liver. Biochim Biophys Acta 483: 46–56PubMedGoogle Scholar
  43. Huseby NE (1979) Subcellular localization of y-glutamyltransferase activity in guinea pig liver. Effect of phenobarbital on the enzyme activity levels. Clin Chim Acta 94: 163–171PubMedCrossRefGoogle Scholar
  44. Idèo G, Tempini S, Bellobuono A, Bellati G, Ronchi G (1980) Biochemical and histochemical estimations of liver y-glutamyltransferase activity in ethanol-fed rats. J Clin Chem Clin Biochem 18: 237–239PubMedGoogle Scholar
  45. Inoue M, Kinne R, Tran T, Biempica L, Arias IM (1983) Rat liver canalicular membrane vesicles. Isolation and topological characterization. J Biol Chem 258: 5183–5188PubMedGoogle Scholar
  46. Ishii H, Okuno F, Shigeta Y, Tsuchiya M (1979) Enhanced serum glutamic oxaloacetic transaminase activity of mitochondrial origin in chronic alcoholics. In: Galanter M (ed) Currents in alcoholism, Vol. V. Grune and Stratton, New York, pp 101–108Google Scholar
  47. Ishii H, Yasuraoka S, Shigeta Y, Takagi S, Kamiya T, Okuno F, Miyamoto K, Tsuchiya M (1978) Hepatic and intestinal gamma-glutamyltranspeptidase activity: its activation by chronic ethanol administration. Life Sci 23: 1393–1398PubMedCrossRefGoogle Scholar
  48. Ishii H, Yasuraoka S, Shigeta Y, Kamiya T, Ebihara Y, Tsuchiya M (1979) Mechanism of enhanced activity of gamma glutamyl transpeptidase ( GGTP) in chronic alcoholics. Alcoholism 3: 180AGoogle Scholar
  49. Ivanov E, Adjarov D, Etarska M, Stankushev T, Brumbarov K, Kerimova M (1980) Elevated liver gamma-glutamyl transferase in chronic alcoholics. Enzyme 25: 304–308PubMedGoogle Scholar
  50. Jenkins WJ, Rosalki SB, Foo Y, Scheuer PJ, Nemesanszky E, Sherlock S (1982) Serum glutamate dehydrogenase is not a reliable marker of liver cell necrosis in alcoholics. J Clin Pathol 35: 207–210PubMedCrossRefGoogle Scholar
  51. Kershenobich D, Garcia-Tsao G, Alvarez Saldana S, Rojkind M (1981) Relationship between blood lactic acid and serum proline in alcoholic liver cirrhosis. Gastroenterology 80: 1012–1015PubMedGoogle Scholar
  52. Kley HK, Both H, Moreno F (1981) Alkohol und Endokrinium. In: Teschke R, Lieber CS (eds) Alkohol und Organschäden. Epidemiologische, klinische, biochemische und therapeutische Aspekte. Witzstrock, Baden-Baden, pp 124–145Google Scholar
  53. Klipstein FA, Lindenbaum J (1965) Folate deficiency in chronic liver disease. Blood 25: 443–456PubMedGoogle Scholar
  54. Köttgen E, Gerok W (1976) Isoenzymdifferenzierung der Gamma-Glutamyltransferase mit Concanavalin A and Con A-Sepharose. Klin Wochenschr 54: 439–444PubMedCrossRefGoogle Scholar
  55. Köttgen E, Reutier W, Gerok W (1978) Induction and ‘superinduction’ of sialylation of membrane-bound y-glutamyltransferase during liver regeneration. Eur J Biochem 82: 279–284PubMedCrossRefGoogle Scholar
  56. Köttgen E, Bauer C, Reutter W, Gerok W (1979) Neue Ergebnisse zur biologischen und medizinischen Bedeutung von Glycoproteinen. 1. Grundlagen und interdisziplinäre Gesichts-punkte. Klin Wochenschr 57: 151–159PubMedCrossRefGoogle Scholar
  57. Köttgen E, Buchsei R, Bauer C (1983) Glycoproteins in liver disease. In: Bianchi L, Gerok W, Landmann L, Sickinger K, Stalder GA (eds) Liver in metabolic diseases. MTP Press, Boston, pp 145–153Google Scholar
  58. Kokot F, Sledzinski Z (1974) Die y-Glutamyltransferase (y-GT). Z Klin Chem Klin Biochem 12: 374–384PubMedGoogle Scholar
  59. Kolb-Bachofen V (1981) Hepatic receptor for asialo-glycoproteins. Ultrastructural demonstration of ligand-induced microaggregation of receptors. Biochim Biophys Acta 645: 293–299PubMedCrossRefGoogle Scholar
  60. Krasner N, Moore MR, Thompson GG, Mcintosh W, Goldberg A (1974) Depression of erythrocyte δ-aminolaevulinic acid dehydratase activity in alcoholics. Clin Sci Mol Med 46: 415–418PubMedGoogle Scholar
  61. Krukenberg S, Nishimura M, Teschke R (1985) Biliary secretion of gamma-glutamyltransferase after chronic alcohol consumption. Submitted for publicationGoogle Scholar
  62. Kryszewski AJ, Neale G, Whitfield JB, Moss DW (1973) Enzyme changes in experimental biliary obstruction. Clin Chim Acta 47: 175–182PubMedCrossRefGoogle Scholar
  63. Kuhn H (1981) Alkohol und Herz. In: Teschke R, Lieber CS (eds) Alkohol und Organschäden. Epidemiologische, klinische, biochemische und therapeutische Aspekte. Witzstrock, Baden- Baden, pp 146–158Google Scholar
  64. Lahrichi M, Ratanasavanh D, Galteau M-M, Siest G (1982) Effect of chronic ethanol administration on gamma-glutamyltransferase activities in plasma and in hepatic plasma membranes of male and female rats. Enzyme 28: 251–257PubMedGoogle Scholar
  65. Lieber CS (1980) Alcohol, protein metabolism, and liver injury. Gastroenterology 79: 373–390PubMedGoogle Scholar
  66. Lieber CS, Jones DP, Mendelson J, DeCarli LM (1963) Fatty liver, hyperlipemia and hyperuricemia produced by prolonged alcohol consumption, despite adequate dietary intake. Trans Assoc Am Physicians 76: 289–300Google Scholar
  67. Lindseil DRM, Wilson AG, Maxwell JD (1982) Fractures on the chest radiograph in detection of alcoholic liver disease. Br Med J 285: 597–599CrossRefGoogle Scholar
  68. Luchi P, Cortis G (1978) Forensic considerations on the comparison of “serum y-glutamyl- transpeptidase” (“y-GT”) activity in experimental acute alcoholic intoxication and in alcoholic car drivers who caused road accidents. Forensic Sci 11: 33–39PubMedCrossRefGoogle Scholar
  69. Malvoisin E, Mercier M, Roberfroid M (1978) Gamma glutamyl transferase. Application of a new radiochemical assay to the analysis of its subcellular distribution in the rat liver. Enzyme 23: 373–381PubMedGoogle Scholar
  70. Mayfield D, McLeod G, Hall P (1974) The CAGE questionnaire: validation of a new alcoholism screening instrument. Am J Psychiatry 131: 1121–1123PubMedGoogle Scholar
  71. Meister A (1973) On the enzymology of amino acid transport. Science 180:33-39 Mills PR, Spooner RJ, Russell RI, Boyle P, MacSween RNM (1981) Serum glutamate dehydrogenase as a marker of hepatocyte necrosis in alcoholic liver disease. Br Med J 283: 754–755Google Scholar
  72. Mills PR, Spooner RJ, Russell RI, Boyle P, MacSween RNM (1981) Serum glutamate dehydrogenase as a marker of hepatocyte necrosis in alcoholic liver disease. Br Med J 283: 754–755CrossRefGoogle Scholar
  73. Moore RA (1972) The diagnosis of alcoholism in a psychiatric hospital: a trial of the Michigan alcoholism screening test (MAST). Am J Psychiatry 128: 1565–1569PubMedGoogle Scholar
  74. Morgan MY, Milsom JP, Sherlock S (1977) Ratio of plasma alpha amino-rc-butyric acid to leucine as an empirical marker of alcoholism: diagnostic value. Science 197: 1183–1185PubMedCrossRefGoogle Scholar
  75. Morgan MY, Camilo ME, Luck W, Sherlock S, Hoffbrand AV (1981a) Macrocytosis in alcohol-related liver disease: its value for screening. Clin Lab Haematol 3: 35–44PubMedGoogle Scholar
  76. Morgan MY, Colman JC, Sherlock S (1981b) The use of a combination of peripheral markers for diagnosing alcoholism and monitoring for continued abuse. Br J Alcohol Alcoholism 16: 167–177Google Scholar
  77. Morland J, Huseby N-E, Sjöblom M, Strömme JH (1977) Does chronic alcohol consumption really induce hepatic microsomal gamma-glutamyltransferase activity? Biochem Biophys Res Commun 77: 1060–1066PubMedCrossRefGoogle Scholar
  78. Naftalin L, Child VJ, Morley DA, Smith DA (1969) Observations on the site of origin of serum y-glutamytranspeptidase. Clin Chim Acta 26: 297–300PubMedCrossRefGoogle Scholar
  79. Nalpas B, Vassault A, Le Guillou A, Lesgourgues B, Ferry N, Lacour B, Berthelot P (1984) Serum activity of mitochondrial aspartate aminotransferase: a sensitive marker of alcoholism with or without alcoholic hepatitis. Hepatology 4: 893–896PubMedCrossRefGoogle Scholar
  80. Nishimura M, Teschke R (1982) Effect of chronic alcohol consumption on the activities of liver plasma membrane enzymes: gamma-glutamyltransferase, alkaline phosphatase and 5’-nucleotid-ase. Biochem Pharmacol 31: 377–381CrossRefGoogle Scholar
  81. Nishimura M, Teschke R (1983) Alcohol and gamma-glutamyltransferase. Klin Wochenschr 61: 265–275PubMedCrossRefGoogle Scholar
  82. Nishimura M, Hasumura Y, Takeuchi J (1980) Effect of an intravenous infusion of ethanol on serum enzymes and lipids in patients with alcoholic liver disease. Gastroenterology 78: 691–695PubMedGoogle Scholar
  83. Nishimura M, Stein H, Berges W, Teschke R (1981) Gamma-glutamyltransferase activity of liver plasma membrane: induction following chronic alcohol consumption. Biochem Biophys Res Commun 99: 142–148PubMedCrossRefGoogle Scholar
  84. Orlowski M, Meister A (1965) Isolation of y-glutamyl transpeptidase from hog kidney. J Biol Chem 240: 338–347PubMedGoogle Scholar
  85. Panteghini M, Falsetti F, Chiari E, Malchiodi A (1983) Determination of aspartate aminotransferase isoenzymes in hepatic diseases-preliminary findings. Clin Chim Acta 128: 133–140PubMedCrossRefGoogle Scholar
  86. Penn R, Worthington DJ (1983) Is serum y-ghitamyltransferase a misleading test? Br Med J 286: 531–535CrossRefGoogle Scholar
  87. Popper H, Reutter W, Gudat F, Köttgen E (1983) Structural carbohydrates in the liver. MTP Press, BostonGoogle Scholar
  88. Ratanasavanh D, Tazi A, Galteau MM, Siest G (1979) Localization of gamma-glutamyltransferase in subcellular fractions of rat and rabbit liver: effect of phenobarbital. Biochem Pharmacol 28: 1363–1365PubMedCrossRefGoogle Scholar
  89. Rauen J, Petrides AS, Teschke R (1980) Anstieg der adulten und fetalen Form der Gamma-Glutamyltransferase(GGT)-Aktivität im Serum bei Patienten mit alkoholischen Leberschäden. Verh Dtsch Ges Inn Med 86: 811–813Google Scholar
  90. Rej R (1978) Aspartate aminotransferase activity and isoenzyme proportions in human liver tissues. Clin Chem 24: 1971–1979PubMedGoogle Scholar
  91. Rej R (1980) An immunochemical procedure for determination of mitochondrial aspartate aminotransferase in human serum. Clin Chim 26: 1694–1700Google Scholar
  92. Reuben A (1984) Biliary proteins. Hepatology 4: 46S–50SPubMedCrossRefGoogle Scholar
  93. Reyes E (1977) Effects of alcohol on the isoenzymes of rat brain y-glutamyl transpeptidase. Proc West Pharmacol Soc 20: 345–349PubMedGoogle Scholar
  94. Reyes E, Miller WR, Taylor CA, Spalding CT (1978) The activity of y-glutamyl transpeptidase in the serum of problem drinkers. Proc West Pharmacol Soc 21: 289–297PubMedGoogle Scholar
  95. Rollason JG, Pincherle G, Robinson D (1972) Serum gamma glutamyl transpeptidase in relation to alcohol consumption. Clin Chim Acta 39: 75–80PubMedCrossRefGoogle Scholar
  96. Ronchi G, Desmet V (1973) Histochemical study of so-called “marker enzymes of cholestasis” during extrahepatic bile duct obstruction in the rat. Beitr Pathol 149: 213–226PubMedGoogle Scholar
  97. Rosalki SB, Rau D (1972) Serum y-glutamyl transpeptidase activity in alcoholism. Clin Chim Acta 39: 41–47PubMedCrossRefGoogle Scholar
  98. Rüssel FGM, Weitering JG, Oosting R, Groothuis GMM, Hardonk MJ, Meijer DKF (1983) Influence of taurocholate on hepatic clearance and biliary excretion of asialo intestinal alkaline phosphatase in the rat in vivo and in isolated perfused rat liver. Gastroenterology 85: 225–234PubMedGoogle Scholar
  99. Rutenburg AM, Kim H, Fischbein JW, Hanker JS, Wasserkrug HL, Seligman AM (1969) Histochemical and ultrastructural demonstration of y-glutamyl transpeptidase activity. J Histochem Cytochem 17: 517–526PubMedCrossRefGoogle Scholar
  100. Ryback RS, Eckardt MJ, Pautler CP (1980) Biochemical and hematological correlates of alcoholism. Res Commun Chem Pathol Pharmacol 27: 533–550PubMedGoogle Scholar
  101. Ryback RS, Eckardt MJ, Felsher B, Rawlings RR (1982) Biochemical and hematologic correlates of alcoholism and liver disease. JAMA 248: 2261–2265PubMedCrossRefGoogle Scholar
  102. Satoh T, Takenaga M, Kitagawa H, Itoh S (1980) Microassay of gamma-glutamyl transpeptidase in needle biopsies of human liver. Res Commun Chem Pathol Pharmacol 30: 151–161PubMedGoogle Scholar
  103. Saunders WM, Kershaw PW (1980) Screening tests for alcoholism - findings from a community study. Br J Addict 75: 37–41PubMedCrossRefGoogle Scholar
  104. Schellenberg B, Arab L, Schlierf G (1982) Alkohol–“Nahrungsmittel” mit Nebenwirkungen. Inn Med 9: 122–125Google Scholar
  105. Schmidt FW (1977) Enzymes in cholestasis. In: Bianchi L, Gerok W, Sickinger K (eds) Liver and bile. MTP Press, Lancaster, pp 203–214Google Scholar
  106. Schrappe O (1981) Alkohol und Nervensystem. In: Teschke R, Lieber CS (eds) Alkohol und Organschäden. Epidemiologische, klinische, biochemische und therapeutische Aspekte. Witz-strock, Baden-Baden, pp 211–224Google Scholar
  107. Schwartz AL, Marshak-Rothstein A, Rup D, Lodish HF (1981) Identification and quantification of the rat hepatocyte asialoglycoprotein receptor. Proc Natl Acad Sei USA 78: 3348–3352CrossRefGoogle Scholar
  108. Seitz HK, Velazquez D, Czygan P, Simon B, Waldherr R, Veith S, Götz R, Kommereil B (1982) Effect of chronic ethanol consumption on duodenal gamma-glutamyltranspeptidase activity (GGT-A) in man and in rats. Scand J Gastroenterol [Suppl 78] 17: 550AGoogle Scholar
  109. Selden C, Wootton AM, Moss DW, Peters TJ (1978) Analytical subcellular fractionation studies on different cell types isolated from normal rat liver. Clin Sci Mol Med 55: 423–427Google Scholar
  110. Selinger MJ, Matloff DS, Kaplan MM (1982) γ-Glutamyl transpeptidase activity in liver disease: serum elevation is independent of hepatic GGTP activity. Clin Chim Acta 125: 283–290PubMedCrossRefGoogle Scholar
  111. Selzer ML (1971) The Michigan alcoholism screening test: the quest for a new diagnostic instrument. Am J Psychiatry 127: 1653–1658PubMedGoogle Scholar
  112. Seymour CA, Peters TJ (1978) Changes in hepatic enzymes and organelles in alcoholic liver disease. Clin Sci Mol Med 55: 383–389PubMedGoogle Scholar
  113. Shaw LM, Marsh E (1981) The effect of one evening of social drinking on serum y-glutamyltransferase activity. Clin Chem 27: 1036AGoogle Scholar
  114. Shaw S, Lieber CS (1978) Plasma amino acid abnormalities in the alcoholic. Respective role of alcohol, nutrition, and liver injury. Gastroenterology 74: 677–682PubMedGoogle Scholar
  115. Shaw S, Lieber CS (1979) Amino acids and ethanol. In: Majchrowicz E, Noble EP (eds) Biochemistry and pharmacology of ethanol, vol 1. Plenum, New York, pp 383–406Google Scholar
  116. Shaw S, Lieber CS (1980a) Increased hepatic production of alpha-amino-n-butyric acid after chronic alcohol consumption in rats and baboons. Gastroenterology 78: 108–113PubMedGoogle Scholar
  117. Shaw S, Lieber CS (1980b) Mechanism of increased gamma glutamyl transpeptidase after chronic alcohol consumption: hepatic microsomal induction rather than dietary imbalance. Subst Alcohol Actions Misuse 1: 423–428PubMedGoogle Scholar
  118. Shaw S, Stimmel B, Lieber CS (1976) Plasma alpha amino-n-butyric acid to leucine ratio: an empirical biochemical marker of alcoholism. Science 194: 1057–1058PubMedCrossRefGoogle Scholar
  119. Shaw S, Lue S-L, Lieber CS (1978) Biochemical tests for the detection of alcoholism: comparison of plasma alpha-amino-n-butyric acid with other available tests. Alcoholism 2: 3–7PubMedGoogle Scholar
  120. Shaw S, Worner TM, Borysow MF, Schmitz RE, Lieber CS (1979) Detection of alcoholism relapse: comparative diagnostic value of MCV, GGTP, and AANB. Alcoholism 3: 297–301PubMedGoogle Scholar
  121. Singer JS, Kaplan MM (1978) ETOH depresses rat liver y-glutamyl transpeptidase. Gastroenterology 74: A-263/1095Google Scholar
  122. Smith GD, Peters TJ (1978) Studies on the subcellular localization of γ-glutamyl transpeptidase in rat liver. Biochem Soc Trans 6: 543–545PubMedGoogle Scholar
  123. Stanko RT, Morse EL, Adibi SA (1977) Effect of chronic alcohol intake on amino acids. Gastroenterology 72: A-113/1136Google Scholar
  124. Stibler H, Borg S (1981) Evidence of a reduced sialic acid content in serum transferrin in male alcoholics. Alcoholism 5: 545–549PubMedGoogle Scholar
  125. Stibler H, Allgulander C, Borg S, Kjellin KG (1978) Abnormal microheterogeneity of transferrin in serum and cerebrospinal fluid in alcoholism. Acta Med Scand 204: 49–56PubMedCrossRefGoogle Scholar
  126. Stibler H, Borg S, Allgulander C (1979) Clinical significance of abnormal heterogeneity of transferrin in relation to alcohol consumption. Acta Med Scand 206: 275–281PubMedCrossRefGoogle Scholar
  127. Stibler H, Sydow O, Borg S (1980) Quantitative estimation of abnormal microheterogeneity of serum transferrin in alcoholics. Pharmacol Biochem Behav [Suppl 1] 13: 47–51CrossRefGoogle Scholar
  128. Szewczuk A, Connell GE (1964) The effect of neuraminidase on the properties of γ-glutamyl transpeptidase. Biochim Biophys Acta 83: 218–223PubMedGoogle Scholar
  129. Tazi A, Gateau M-M, Siest G (1980) γ-Glutamyltransferase of rabbit liver: kinetic study of phenobarbital induction and in vitro solubilization by bile salts. Toxicol Appl Pharmacol 55: 1–7PubMedCrossRefGoogle Scholar
  130. Teschke R (1980) Leberschäden durch Alkohol: biochemische und klinische Studien zur Pathogenese und Früherkennung. Thieme, StuttgartGoogle Scholar
  131. Teschke R (1981) Alkohol und Leber. In: Teschke R, Lieber CS (eds) Alkohol und Organschäden. Epidemiologische, klinische, biochemische und therapeutische Aspekte. Witzstrock, Baden- Baden, pp 89–110Google Scholar
  132. Teschke R, Petrides AS (1982) Hepatic gamma-glutamyltransferase activity: its increase following chronic alcohol consumption and the role of carbohydrates. Biochem Pharmacol 31: 3751–3756PubMedCrossRefGoogle Scholar
  133. Teschke R, Brand A, Strohmeyer G (1977) Induction of hepatic microsomal gamma-glutamyltransferase activity following chronic alcohol consumption. Biochem Biophys Res Commun 75: 718–724PubMedCrossRefGoogle Scholar
  134. Teschke R, Rauen J, Neuefeind M, Petrides AS, Strohmeyer G (1980) Alcoholic liver disease associated with increased gamma-glutamyltransferase activities in serum and liver. In: Thurman RG (ed) Alcohol and aldehyde metabolizing systems — 4. Plenum, New York, pp 647–654 (Advances in experimental medicine and biology, vol 132 )Google Scholar
  135. Teschke R, Neuefeind M, Nishimura M, Strohmeyer G (1983) Hepatic gamma-glutamyltransferase activity in alcoholic fatty liver: comparison with other liver enzymes in man and rats. Gut 24: 625–630PubMedCrossRefGoogle Scholar
  136. Teschke R, Krukenberg S, Nishimura M, Stremmel W, Strohmeyer G (1984) Diskrete Cholestase nach chronischem Alkoholkonsum als Ursache fur die Aktivitatssteigerung der Gamma-Glutamyltransferase? Z Gastroenterologie 22: 494Google Scholar
  137. Teschke R, Krukenberg S, Stremmel W, Nishimura M (1985) Alcohol, discrete cholestasis and gamma-glutamyltransferase: an experimental study in rats. Submitted for publicationGoogle Scholar
  138. Tobiasson P, Boeryd B (1980) Serum cholic and chenodeoxycholic acid conjugates and standard liver function tests in various morphological stages of alcoholic liver disease. Scand J Gastroenterol 15: 657–663PubMedCrossRefGoogle Scholar
  139. Uchida T, Peters RL (1983) The nature and origin of proliferated bile ductules in alcoholic liver disease. Am J Clin Pathol 79: 326–333PubMedGoogle Scholar
  140. Unger KW, Johnson D Jr (1974) Red blood cell mean corpuscular volume: a potential indicator of alcohol usage in a working population. Am J Med Sci 267: 281–289PubMedCrossRefGoogle Scholar
  141. van Waes L, Lieber CS (1977) Glutamate dehydrogenase: a reliable marker of liver cell necrosis in the alcoholic. Br Med J 2: 1508–1510PubMedCrossRefGoogle Scholar
  142. Wadstein J, Skude G (1979) Serum ethanol, hepatic enzymes and length of debauch in chronic alcoholics. Acta Med Scand 205: 317–318PubMedCrossRefGoogle Scholar
  143. Weill J, Schellenberg F, LeGoff A-M, Lamy J (1982) The predictive value of gamma-glutamyl transferase and other peripheral markers in the screening of alcohol abuse. In: Siest G, Heusghem C (eds) Gammaglutamyltransferases: advances in biochemical pharmacology, 3rd series. Masson, Paris, pp 195–198Google Scholar
  144. Whitehead TP, Clarke CA, Whitfield AGW (1978) Biochemical and haematological markers of alcohol intake. Lancet 1: 978–981PubMedCrossRefGoogle Scholar
  145. Whitfield JB, Hensley WJ, Bryden D, Gallagher H (1978) Estimation of alcohol intake from laboratory results. Ann Clin Biochem 15: 304–306PubMedGoogle Scholar
  146. Widnell CC (1972) Cytochemical localization of 5’-nucleotidase in subcellular fractions isolated from rat liver. I. The origin of 5’-nucleotidase activity in microsomes. J Cell Biol 52: 542–558PubMedCrossRefGoogle Scholar
  147. Wienbeck M, Berges W (1981) Oesophageal lesions in the alcoholic. Clin Gastroenterol 10: 375–388PubMedGoogle Scholar
  148. Wootton AM, Neale G, Moss DW (1977) Enzyme activities of cells of different types isolated from livers of normal and cholestatic rats. Clin Sci Mol Med 52: 585–590PubMedGoogle Scholar
  149. Worner TM, Lieber CS (1980) Plasma glutamate dehydrogenase: clinical application in patients with alcoholic liver disease. Alcoholism 4: 431–434PubMedGoogle Scholar
  150. Wu A, Chanarin I, Levi AJ (1974) Macrocytosis of chronic alcoholism. Lancet 1: 829–830PubMedGoogle Scholar
  151. Wu A, Slavin G, Levi A J (1976) Elevated serum gamma-glutamyl-transferase (transpeptidase) and histological liver damage in alcoholism. Am J Gastroenterol 65: 318–323PubMedGoogle Scholar
  152. Yamauchi M, Kimura K, Kawase H, Watanabe Y, Kitahara T, Ogura K, Fujisawa K (1984) Hepatic gamma-glutamyl transferase and hepatic fibrosis in patients with alcoholic liver disease. Enzyme 32: 110–115PubMedGoogle Scholar
  153. Zeitlin PL, Hubbard AL (1982) Cell surface distribution and intracellular fate of asialoglycoproteins: a morphological and biochemical study of isolated rat hepatocytes and monolayer cultures. J Cell Biol 92: 634–647PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • R. Teschke
    • 1
  1. 1.Medizinische Klinik DUniversität DüsseldorfDüsseldorf 1Federal Republic of Germany

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