Quantitative Analysis of Plant Membrane Lipids by a Combined TLC-GLC Procedure

  • W. Eichenberger

Abstract

In the analysis of membrane acyl lipids two aspects are very often of major interest: The quantitative determination of the particular lipid components and their fatty acid composition. The scope of the experiment is the separation of a lipid mixture by two-dimensional TLC and the subsequent separation and quantitative determination by GLC of the fatty acids. From the total amount of fatty acids, the amount of particular lipid components is calculated.

Keywords

Chlorophyll Petroleum Hexane Chloroform MeOH 

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References

  1. Allen CF, Good P (1971) In: Colowick SP and Kaplan NO (eds) Methods in Enzymology, Vol 23A, Elsevier, New York, pp 523–547Google Scholar
  2. Thies W (1971) Z. Pflanzenzucht 65: 181–202Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • W. Eichenberger

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