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Quantitative Analysis of Plant Membrane Lipids by a Combined TLC-GLC Procedure

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Abstract

In the analysis of membrane acyl lipids two aspects are very often of major interest: The quantitative determination of the particular lipid components and their fatty acid composition. The scope of the experiment is the separation of a lipid mixture by two-dimensional TLC and the subsequent separation and quantitative determination by GLC of the fatty acids. From the total amount of fatty acids, the amount of particular lipid components is calculated.

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References

  • Allen CF, Good P (1971) In: Colowick SP and Kaplan NO (eds) Methods in Enzymology, Vol 23A, Elsevier, New York, pp 523–547

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  • Thies W (1971) Z. Pflanzenzucht 65: 181–202

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© 1984 Springer-Verlag Berlin Heidelberg

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Eichenberger, W. (1984). Quantitative Analysis of Plant Membrane Lipids by a Combined TLC-GLC Procedure. In: Azzi, A., Brodbeck, U., Zahler, P. (eds) Enzymes, Receptors, and Carriers of Biological Membranes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70010-1_4

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  • DOI: https://doi.org/10.1007/978-3-642-70010-1_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-13751-1

  • Online ISBN: 978-3-642-70010-1

  • eBook Packages: Springer Book Archive

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