Fluorescent Labeling of Band 3 Protein from Erythrocytes
Fluorescence spectroscopy has become a valuable tool in the study of membrane proteins. Measurements of the fluorescence of extrinsic probes as well as of that intrinsic to the proteins have provided a considerable insight into the structure and mobility of these systems. The use of fluorescent probes to study membrane proteins is illustrated in the practical exercise described here, where the interaction of the mercurial reagent fluorescein mercuric acetate with Band 3 protein of erythrocytes is examined.
KeywordsExcited Singlet State Polarizer Horizontal Practical Exercise Vibrational Energy Level Fluorescent Energy Transfer
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