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Analytical Use of Luminous Bacteria in Veterinary and Medical Microbiology

  • S. Ulitzur
  • A. Naveh
  • M. Barak
Conference paper

Abstract

The use of intact luminous bacteria for analytical purposes has some clear advantages. Intact luminous bacteria are self-maintaining luminescent units and the in vivo luminescence of a single bacterium can be detected with the aid of a scintillation counter. In addition to naturally occurring species of luminous bacteria (Photobacterium and Vibrio), we cloned the luminescent system into different gram-negative bacteria, including Escherichia coli and Salmonella typhimurium. The luminous bacteria may be lyophilized and stored for many months in a cold room. Such lyophilized cultures promptly resume their full level of in vivo luminescence upon hydration.

Keywords

Viable Count Bioluminescence Assay Luminous Bacterium Serum Bactericidal Activity Luminescent System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Naveh A, Potasman I, Basan M, Ulitzur S (1983) A new rapid and sensitive bioluminescence assay for antibiotics that inhibit protein synthesis. J Appl Bacteriol 56:457–462Google Scholar
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    Barak M, Ulitzur S, Merzbach D (1983) Elucidation of the phagocytosis mechanism with the aid of luminous bacteria. J Med Microbiol 18:65–72CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • S. Ulitzur
  • A. Naveh
  • M. Barak
    • 1
  1. 1.Department of Food Engineering and BiotechnologyIsrael Institute of Technology, Technion CityHaifaIsrael

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