Short-Term Methods for Isolation and Enumeration of Bacteria in the Respiratory Tract
Interest in quantitative respiratory bacteriology derives from the opinion that, as in other clinical specimens, e.g., urine, quantitative data may have a pathogenetic value in the complete and quick diagnosis of certain bacterial bronchopneumo-pathies. The concentration of a single bacterial species isolated from sputum, tracheoaspirates, bronchoaspirates, or other bronchial material, at least in some cases, could allow some real difficulties in respiratory bacteriology, such as the contamination by oral microflora, to be surmounted. In fact, with this method it is easier to detect the species which has a pathogenetic role in a bronchopneu-mopathy, because this speciesis more numerous than “colonizing” bacteria of the respiratory tract. Furthermore, the same quantitative examination allows the ratios of the different bacterial species to be studied, thus providing an exact map of the complex bacterial ecology of the respiratory tract.
KeywordsClinical Specimen Bacterial Concentration Agar Dilution Method Quantitative Examination Streptococcus Viridans
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