Abstract
A major application of chromatography in microbiology has been the study of the chemical composition of microorganisms. Data from these studies have demonstrated the value of chemical information for differentiation of closely related groups or species of microorganisms. The chemical classes studied in greatest detail are cellular fatty acids (and other lipids) and respiratory quinones. Methodology and chromatographic techniques for determination of these compounds and examples of their usefulness in taxonomy are discussed in this report.
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References
Aluyi HS, Drucker DB (1983) Trimethylsilyl-sugar profiles of Streptococcus milleri and Streptococcus mitis. J Appl Bacteriol 54:391–397
Alvin C, Larsson L, Magnusson M, Mardh PA, Odham G, Westerdahl G (1983) Determination of fatty acids and carbohydrate monomers in micro-organisms by glass capillary gas chromatography: analysis of Mycobacterium gordonae and Mycobacterium scrofulaceum. J Gen Microbiol 129:401–405
Becker B, Lechevalier MP, Gordon RE, Lechevalier HA (1964) Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol 12:421–423
Berd D (1973) Laboratory identification of clinically important aerobic actinomycetes. Appl Microbiol 25:665–681
Carlone GM, Anet FAL (1983) Detection of menaquinone-6 and a novel methyl-substituted menaquinone- 6 in Campylobaeter jejuni and Campylobaeter fetus subsp etus. J Gen Microbiol 129:3385–3393
Collins MD (1982) A note on the separation of natural mixtures of bacterial menaquinones using reverse-phase high-performance liquid chromatography. J Appl Bacteriol 52:457–460
Collins MD, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354
Ferragut C, Izard D, Gavini F, Kersters K, Delay J, Leclere H (1983) Klebsiella trevisonii: a new species from water and soil. Int J Syst Bacteriol 33:133–142
Fox A, Morgan SL, Hudson JR, Zhu ZT, Lau PY (1983) Capillary gas chromatographic analysis of alditol acetates of neutral and amino sugars in bacterial cell walls. J Chromatogr 256:429–438
Hanna J, Neill SD, O’Brien JJ, Ellis WA (1983) Comparison of aerotolerant and reference strains of Campylobacter species by polyacrylamide gel electrophoresis. Int J Syst Bacteriol 33:143–146
Jantzen E, Berdal BP, Omland T (1979) Cellular fatty acid composition of Francisella tularensis. J Clin Microbiol 10:928–930
Karr DE, Bibb WF, Moss CW (1982) Isoprenoid quinones of the genus Legionella. J Clin Microbioi 15:1044–1048
Lambert MA, Moss CW (1983) Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria. J Clin Microbiol 18:1370–1377
Lechevalier MP (1982) Lipids in bacterial taxonomy. In: Laskin AI, Lechevalier HA (eds) CRC handbook of microbiology, 2nd edn. CRC, Boca Raton, pp 436–508
Lechevalier MP, Lechevalier H, Horan AC (1973) Chemical characteristics and classification of nocardiae. Can J Microbiol 19:965–972
Mayberry WR, Lambe DW, Ferguson KP (1982) Identification of Bacteroides species by cellular fatty acid profiles. Int J Syst Bacteriol 32:21–27
Minnikin DE, Goodfellow M (1980) Lipid composition in the classification and identification of acid-fast bacteria. In: Goodfellow M, Board RG (eds) Microbiological classification and identification. Academic, London, p 189
Moore WEC, Hash DE, Holdeman LV, Cato EP (1980) Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl Environ Microbiol 39:900–907
Moss CW (1981) Gas-liquid chromatography as an analytical tool in microbiology. J Chromatogr 203:337–347
Moss CW, Bibb WF, Karr DE, Guerrant GO, Lambert MA (1983) Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov. J Clin Microbiol 18:917–919
Moss CW, Guerrant GO (1983) Separation of bacterial ubiquinones by reverse-phase high performance liquid chromatography. J Clin Microbiol 18:15–17.
Moss CW, Kai A, Lambert MA, Patton C (1984) Isoprenoid quinone content and cellular fatty acid composition of Campylobacter. J Clin Microbiol 19:772–776
Moss CW, Weaver RE, Dees SB, Cherry WB (1977) Cellular fatty acid composition of isolates from Legionnaires’ disease. J Clin Microbiol 6:140–143
Prichard DG, Coligan JE, Speed SE, Gray BM (1981) Carbohydrate fingerprints of streptococcal cells. J Clin Microbiol 13:89–92
Regnier FE (1983) HPLC of proteins, peptides, and polynucleotides. Anal Chem 55:1299A–1306A
Shaw N (1974) Lipid composition as a guide to the classification of bacteria. Adv Appl Microbiol 17:63–108
Tamoaka J, Katayoma-Fujimura Y, Kuraishi H (1983) Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36
Wilkinson SG, Carby KA (1971) Amino sugars in the cell walls of Pseudomonas species. J Gen Microbiol 66:221–227
Yamada Y, Inouye G, Tahara Y, Kondo K (1976) The menaquinone system in the classification of coryneform and nocardioform bacteria and related organisms. J Gen Appl Microbiol 22:203–214
Yabuuchi E, Moss CW (1982) Cellular fatty acid composition of strains of three species of Sphingobacterium gen. nov. and Cytophagajohnsonae. FEMS Microbiol Lett 13:87–91
Yabuuchi E, Kaneko T, Yano I, Moss CW, Miyoshi M (1983) Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose-nonfermenting gramnegative rods in CDC groups IIk-2 and IIb. Int J Syst Bacteriol 33:580–598
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© 1985 Springer-Verlag Berlin Heidelberg
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Moss, C.W. (1985). Chromatographic Analysis of Bacterial Cellular Components. In: Habermehl, KO. (eds) Rapid Methods and Automation in Microbiology and Immunology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69943-6_31
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DOI: https://doi.org/10.1007/978-3-642-69943-6_31
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69945-0
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