Problems of Rubella Diagnosis by Various IgM Techniques and the Need for Test Combinations

  • G. Enders


The role of specific IgM antibodies as a valuable marker was recognized for the diagnosis of acute postnatal and congenital rubella infections more than 15 years ago. Today IgM and/or IgA antibody detection plays a leading role in the diagnosis of a number of viral and other microbial infections. In no other infection, however, is the correctness of the IgM antibody test results of such importance as for the detection of acute rubella infection in pregnancy. An infant with congenital rubella syndrome is only one of the possible outcomes of infection in a pregnant woman. Therapeutic abortion is another serious consequence.


Rubella Virus Congenital Rubella Syndrome Rubella Infection Rubella Antibody Congenital Rubella Infection 
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  1. 1.
    Best JM, Palmer SJ, Morgan-Capner P, Hodgson J (1984) A comparison of Rubazyme-M and MACRIA for the detection of rubella-specific IgM. J Virol Methods 8:99–109PubMedCrossRefGoogle Scholar
  2. 2.
    Cradock-Watson JE, Ridehalgh MKS, Pattison JR, Anderson MJ, Kangro HO (1979) Comparison of immunofluorescence and radioimmunoassay for detecting IgM antibody in infants with the congenital rubella syndrome. J Hyg (Lond) 83:413–425CrossRefGoogle Scholar
  3. 3.
    Diment JA, Chantler SM (1981) Enzyme immunoassay for detection of rubella-specific IgM antibody. Lancet I:394–395CrossRefGoogle Scholar
  4. 4.
    Enders G (1984) Rubella infections. Med Lab (Stuttg) [Suppl] I:58–71Google Scholar
  5. 5.
    Enders G (1985) Serological test combinations for safe detection of rubella infections. (Intern. Symposium on the Prevention of Congenital Rubella in Washington 13. −14.3.84). Rev Infect Dis 7 [Suppl 1] (to be published)Google Scholar
  6. 6.
    Enders G, Calm A, Schaub J (1984) Rubella embryopathy after previous maternal rubella vaccination. Infection 12:96–98PubMedCrossRefGoogle Scholar
  7. 7.
    Enders G, Knotek F (1985) Comparison of the performance and reproducibility of various serological methods and diagnostic kits for the detection of rubella antibodies. J Virol Meth (to be published)Google Scholar
  8. 8.
    Enders G, Knotek F, Greeck A (1985) Comparison of negative, low-positive and positive hemagglutination inhibition rubella antibody titers by single radial hemolysis test, ELISA Enzygnost IgG and ELISA Rubazyme IgG. J Virol Meth (submitted for publication)Google Scholar
  9. 9.
    Enders G, Knotek F, Pacher U (1985) Comparison of various serological methods and diagnostic kits for the detection of acute, recent and previous rubella infection, vaccination and congenital infections. J Med Virol 12 (to be published)Google Scholar
  10. Enders G (1985) Detection of IgM antibodies against Rubella virus: Comparison of two indirect ELISAS and an anti-my-EIA. J Med Virol (to be published)Google Scholar
  11. 10.
    Enders G, Biber M (1985) Reactivity for rubella- and cytomegalo-specific IgM in sera from patients with infectious mononucleosis. J Clin Microbiol (submitted for publication)Google Scholar
  12. 11.
    Gupta JD, Peterson V, Stont M, Murphy AM (1971) Single sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column. J Clin Pathol 24:547–550PubMedCrossRefGoogle Scholar
  13. 12.
    Huschka U, Maier J, Rautenberger EW, Doerr HW (1982) Rapid separation of immunoglobulin M by immunoaffinity chromatography for detection of specific antibodies to Rubella and Treponema pallidum. Eur J Clin Microbiol 1:18–121CrossRefGoogle Scholar
  14. 13.
    Isaac M, Payne RA (1982) Antibody class capture assay (ACCA) for rubella-specific IgM antibody. J Med Virol 10:55–64PubMedCrossRefGoogle Scholar
  15. 14.
    Kalimo KOK, Meurman OH, Halonen PE, Ziola BR, Viljanen MK, Granfors K, Toivanen P (1976) Solid-phase radioimmunoassay of Rubella virus immunoglobulin G and immunoglobulin M antibodies. J Clin Microbiol 4: 117–123PubMedGoogle Scholar
  16. 15.
    Krech U, Wilhelm JA (1979) A solid-phase immunosorbent technique for the rapid detection of rubella IgM by haemagglutination inhibition. J Genet Virol 44:281CrossRefGoogle Scholar
  17. 16.
    Kurtz JB, Malic A (1981) Rubella-specific IgM detected by an antibody capture assay/ELISA technique. J Clin Pathol 34:1392–1395PubMedCrossRefGoogle Scholar
  18. 17.
    Logt JTH van der, Loon AM van, Veen J van der (1981) Hemadsorption immunosorbent technique for determination of Rubella immunoglobulin M antibody. J Clin Microbiol 13:410–415PubMedGoogle Scholar
  19. 18.
    Luton P, Ridgway GL (1979) Use of the ultracentrifuge vertical rotor in the detection of rubella specific IgM on a sucrose density gradient. J Clin Pathol 32:931PubMedCrossRefGoogle Scholar
  20. 19.
    Meurman OH, Viljanen MK, Granfors K (1977) Solidphase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation test. J Clin Microbiol 5:257PubMedGoogle Scholar
  21. 20.
    Morgan-Capner P (1984) The detection of rubella-specific antibody. PHLS Microbiol Digest 1:611Google Scholar
  22. 21.
    Morgan-Capner P, Davies E, Pattison JR (1980) Rubella-specific IgM detection using Sephacryl S-300 gel filtration. J Clin Pathol 33:1082–1085PubMedCrossRefGoogle Scholar
  23. 22.
    Morgan-Capner P, Tedder RS, Mace JE (1983) Rubella-specific IgM reactivity in sera from cases with infectious mononucleosis. J Hyg (Cambr) 90:407–413CrossRefGoogle Scholar
  24. 23.
    Mortimer PP, Tedder RS, Hambling MH, Shafi MS, Burkhardt F, Schild U (1981) Antibody capture radioimmunoassay for anti-rubella IgM. J Hyg (Cambr) 86:139–153CrossRefGoogle Scholar
  25. 24.
    National External Quality Assessment Scheme (NEQAS) (1982) Operated by the PHLS Division of Microbiological Reagents and Quality Control, Central Public Health LaboratoryHBK. Rubella serology IgM. Distribution No. 224Google Scholar
  26. 25.
    Pattison IR (1982) Laboratory investigation of rubella. In: Public Health Laboratory Service Monograph, Series No. 16Google Scholar
  27. 26.
    Sann G, Schneider G, Loeke S, Doerr HW (1983) Rapid fractionation of serum immunoglobulins by high pressure liquid gel permeation chromatography. Application to routine serologic procedures. J Immunol Methods 59:121–127Google Scholar
  28. 27.
    Tedd er RS, Yao JL (1982) The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella-specific IgM. J Hyg (Camb) 88:335–350CrossRefGoogle Scholar
  29. 28.
    Vejtorp M (1981) Solid phase anti-IgM ELISA for detection of rubella specific IgM antibodies. Acta Path Microbiol Scand B 89:123–128Google Scholar
  30. 29.
    Vesikari T, Vaheri A (1968) Rubella: A method for rapid diagnosis of recent infection by demonstration of the IgM antibodies. Br Med J 2:221–223CrossRefGoogle Scholar
  31. 30.
    Waxham MN, Wolinsky JS (1983) Immunochemical identification of Rubella virus hemagglutinin. Virology 126:194–203PubMedCrossRefGoogle Scholar
  32. 31.
    Waxham MN, Wolinsky JS (1985) A model of the structural organization of rubella virions. (Intern. Symposium on the Prevention of Congenital Rubella, Washington, 13.-14.3.84) Rev Infect Dis 7 [Suppl 1] (to be published)Google Scholar
  33. 32.
    Ziegelmaier R, Behrens F, Enders G (1980) ELISA: Demonstration of IgG and IgM antibodies in infections with Cytomegalo (CMV) and Rubella virus. Med Lab (Stuttg) 8:16–25Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • G. Enders
    • 1
  1. 1.Virologisch-med. Diagnostisches InstitutStuttgart 1Germany

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