Immunoblotting and Dot Immunobinding
The great advances in immunoassay technique in recent years have resulted from the advent of solid-phase methodology. The use of reactants bound to a solid phase permits the easy removal of excess reagents in simple, rapid washing procedures. The resulting methodology is rapid, simple, and quantitative. However, this methodology does not as such give directly qualitative information concerning homogeneity, purity, or identity of immunoreacting species. Classic immuno-diffusion and immunoelectrophoresis, depending on diffusion and precipitation in a gel phase, did indeed yield such qualitative information. However, these methods are slow, cumbersome, and insensitive. With the advent of immunoblotting (Towbin et al. 1979), it has been possible to combine the power of the classic methodology for qualitative analysis with the speed, simplicity, and sensitivity of solid-phase methodology.
KeywordsRibosomal Protein Connective Tissue Disease Mixed Connective Tissue Disease Patient Seron Allergy Diagnosis
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