Abstract
Nucleic acid hybridization is based on the tendency of nucleic acids to form double-stranded hydrogen-bonded complexes if strands of complementary sequences are incubated under appropriate salt and temperature conditions. The technique has been widely applied to study mainly the following questions:
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Presence of genes
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State of genes (free linear, circular, integrated)
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Localization of genes in specific tissue or cell types or in subcellular structures
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Transcriptional activity of genes.
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References
Alwine J, Kemp D, Stark G (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74/12: 5350–5354
Botchan M, Topp W, Sambrook J (1976) The arrangement of simian virus 40 sequences in the DNA of transformed cell. 9: 269
Brandsma J, Müller G (1980) Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA. Proc Natl Acad Sci USA 77: 6851–5
Bresser J. Doering J. Gillespie D (1983) Quick-blot: Selective mRNA or DNA immobilization from whole cells. DNA 2: 3
Brigati D, Myerson D, Leary J, Spalholz B, Travis S, Fong C, Hsiung G, Ward D (1983) Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology 126: 32–50
Britten R, Smith J (1970) A bovine genome. Carnegie Inst Washington Yearb 68: 378: 386
Chou S, Merigan T (1983) Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization. N Engl J Med 308/16: 921
Commerford S (1971) Iodination of nucleic acids in vitro. Biochemistry 10/10: 1993–1999
Desgranges C, Wolf H, De-The G, Shanmugaratnam K, Cammoun N, Ellouz R, Klein G, Lennert K, Munez N, Zurhausen H (1975) Nasopharyngeal carcinoma, X. Presence of Epstein-Barr genomes in separated epithelial cells of tumors in patients from Singapore, Tunisia and Kenya. Int J Cancer 16: 7–15
Desgranges C, Bornkamm G, Zeng Y, Wang P, Zhu J, Shang M, De-The G (1982) Detection of Epstein-Barr viral DNA internal repeats in the nasopharyngeal mucosa of chinese with IgA/EBV-specific antibodies. Int J Cancer 29: 87–91
Flores J, Purcell R, Perez I, Wyatt R, Boeggeman E, Sereno M, White L, Chanock R, Kapikian A (1983) A dot hybridisation assay for detection of rotavirus. Lancet 1: 555–558
Frenkel N, Locker H, Cox B, Roizman B, Rapp F (1976) Herpes simplex virus DNA in transformed cells: Sequence complexity in five hamster cell lines and one derived hamster tumor. J Virol 18/3: 885–893
Gall J, Pardue J (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. DNAS 63: 378–383
Gerhard D, Kawasaki E, Bancroft F, Szabo P (1981) Localization of a unique gene by direct hybridization in situ. Proc Natl Acad Sci USA 78/6: 3755–3759
Gillespie D, Spiegelman S (1965) A quantitative assay for DNA/RNA hybrids with DNA immobilized on a membrane. J Mol Biol 12: 829
Gillis M, De Ley J, De Cleene M (1970) The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12: 143–153
Grunstein M, Hogness D (1975) Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc Nat! Acad Sci 72/10: 3961–3965
Gu S, Wolf H, Yi Z (1983) Cloning fragments of EBV-DNA in single-stranded phage M13mp8 I preparation and identification of cloned DNA. Cancer (China) 129–135
Han J, Harding J (1983) Using iodinated single-stranded M13 probes to facilitate rapid DNA sequence analysis-nucleotide sequence of a mouse lysine tRNA gene. Nucleic Acids Res 11: 7
Henderson A, Ripley S, Heller M, Kieff E (1983) Chromosome site for Epstein-Barr virus DNA in a Burkitt tumor cell line and in lymphocytes growth-transformed in vitro. Proc Natl Acad Sci USA 80: 1987–1991
Howley P, Israel M, Law M, Martin M (1979) Rapid methods for detecting and mapping homology between heterologous DNAs. J Biol Chem 254/10: 4876–4883
Hu N, Messing J (1982) The making of strand-specific M13 probes. Gene 17: 271–277
Kohne D, Britten R (1971) Hydroxyapatit techniques for nucleic acid reassociations. In: Davies (ed) Procedures in nucleic Acid Research, vol 2. Harper and Rowe, New York, pp 500–512
Langer P, Waldrop A, Ward D (1981) Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78/11: 6633–6637
Langridge J, Langridge P, Bergquist P (1980) Extraction of nucleic acids from agarose gels. Anal Bio-chem 103: 264–271
McConaughy L, Laird C, McCarthy B (1969) Nucleic acid reassociation in formamide. Biochemistry 8: 8
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101: 20
Peden K, Mounts P, Hayward G (1982) Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high-complexity probe. Cell 31: 71–80
Prensky W (1976) The radioiodination of RNA and DNA to high specific activities. Methods Cell Biol 13: 121–152
Richter W, Gu S, Seibl R, Wolf H (1983) A new method for examination of carcinomas of the nasopharynx. In: Prasad U et al. (eds) Nasopharyngeal carcinoma: Current concepts. University of Malaya Press, pp 25–33
Scotto J, Hadchouel M, Hery C, Yvart J, Tiollais P, Brechot C (1983) Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: Comparison with results for other viral markers. Hepatology 3/3: 279: 284
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517
Thomas P (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77/9: 5201–5205
Vincent M, Beltz W, Ashton S (1982) Preparation of DNA labeled with high specific activity S35-deoxy-adenosine 5’ (a-thio) triphosphate; the use of 35S-Labeled nucleic acids as molecular hybridization probes. Miami Winter Symposium
Vogelstein B, Gillespie D (1979) Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci USA 76/2: 615: 619
Wolf H (1981) Die Verwendung verschiedener Nukleinsäure-Hybridisierungstechniken am Beispiel von Epstein-Barr-Virus korrelierter Erkrankungen. Verh Dtsch Ges Pathol 65: 47–57
Wolf H, zurHausen H, Becker V (1973) EB viral genomes in epithelial nasopharyngeal carcinoma cells. Nature New Biol 138: 245–247
Wolf H, zurHausen H, Klein G, Becker V, Henle G, Henle W (1975) Attempts to detect virus-specific DNA sequences in human tumors, III. Epstein-Barr viral DNA in non-lymphoid nasopharyngeal carcinoma cells. Med Microbiol Immunol (Berl) 161: 15–21
Wolf H, Haus M, Wilmes E (1984) EBV persists in the parotid gland. J Virol 51: 795–798
zurHausen H, Diehl V, Wolf H, Schulte-Holthausen H (1974) EB virus associated macromolecules in cells derived from human tumors. Mol Studies Viral Neoplasia 517–530
zurHausen H, Schulte-Holthausen H, Klein G, Henle W, Henle G, Clifford P, Santesson L (1970) EBV DNA in biopsies of Burkitt tumors and anaplastic carcinomas of the nasopharynx. Nature 228: 1056–1058
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© 1985 Springer-Verlag Berlin Heidelberg
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Wolf, H. et al. (1985). New Developments in Nucleic Acid Hybridization. In: Habermehl, KO. (eds) Rapid Methods and Automation in Microbiology and Immunology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69943-6_11
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DOI: https://doi.org/10.1007/978-3-642-69943-6_11
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