New Developments in Nucleic Acid Hybridization
Nucleic acid hybridization is based on the tendency of nucleic acids to form double-stranded hydrogen-bonded complexes if strands of complementary sequences are incubated under appropriate salt and temperature conditions. The technique has been widely applied to study mainly the following questions:
Presence of genes
State of genes (free linear, circular, integrated)
Localization of genes in specific tissue or cell types or in subcellular structures
Transcriptional activity of genes.
KeywordsNasopharyngeal Carcinoma Nucleic Acid Hybridization Spot Hybridization Nucleic Acid Spot Hybridization Free Linear
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Unable to display preview. Download preview PDF.
- Bresser J. Doering J. Gillespie D (1983) Quick-blot: Selective mRNA or DNA immobilization from whole cells. DNA 2: 3Google Scholar
- Britten R, Smith J (1970) A bovine genome. Carnegie Inst Washington Yearb 68: 378: 386Google Scholar
- Desgranges C, Wolf H, De-The G, Shanmugaratnam K, Cammoun N, Ellouz R, Klein G, Lennert K, Munez N, Zurhausen H (1975) Nasopharyngeal carcinoma, X. Presence of Epstein-Barr genomes in separated epithelial cells of tumors in patients from Singapore, Tunisia and Kenya. Int J Cancer 16: 7–15PubMedCrossRefGoogle Scholar
- Gu S, Wolf H, Yi Z (1983) Cloning fragments of EBV-DNA in single-stranded phage M13mp8 I preparation and identification of cloned DNA. Cancer (China) 129–135Google Scholar
- Kohne D, Britten R (1971) Hydroxyapatit techniques for nucleic acid reassociations. In: Davies (ed) Procedures in nucleic Acid Research, vol 2. Harper and Rowe, New York, pp 500–512Google Scholar
- Langridge J, Langridge P, Bergquist P (1980) Extraction of nucleic acids from agarose gels. Anal Bio-chem 103: 264–271Google Scholar
- Richter W, Gu S, Seibl R, Wolf H (1983) A new method for examination of carcinomas of the nasopharynx. In: Prasad U et al. (eds) Nasopharyngeal carcinoma: Current concepts. University of Malaya Press, pp 25–33Google Scholar
- Scotto J, Hadchouel M, Hery C, Yvart J, Tiollais P, Brechot C (1983) Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: Comparison with results for other viral markers. Hepatology 3/3: 279: 284Google Scholar
- Vincent M, Beltz W, Ashton S (1982) Preparation of DNA labeled with high specific activity S35-deoxy-adenosine 5’ (a-thio) triphosphate; the use of 35S-Labeled nucleic acids as molecular hybridization probes. Miami Winter SymposiumGoogle Scholar
- Wolf H, zurHausen H, Becker V (1973) EB viral genomes in epithelial nasopharyngeal carcinoma cells. Nature New Biol 138: 245–247Google Scholar
- zurHausen H, Diehl V, Wolf H, Schulte-Holthausen H (1974) EB virus associated macromolecules in cells derived from human tumors. Mol Studies Viral Neoplasia 517–530Google Scholar
© Springer-Verlag Berlin Heidelberg 1985