The Use of Whole Embryo Culture in the Study of Teratogenic Mechanisms
Direct exposure of the embryo to the teratogen. This will reveal the direct effect of an agent independent of any interaction with the maternal metabolism.
Addition of the agent with drug metabolising systems. This is a method of re-adding drug metabolism by incorporation of microsomes and co-factors or intact, isolated hepatocytes. It is analogous to the Ames test.
Serum from a treated or deficient individual as the culture medium. This procedure re-adds the effects of maternal metabolism and has greater similarity to in vivo exposure. Human serum can be used.
In vivo treatment with in vitro evaluation — pregnant rats are dosed with the teratogen. The embryos are subsequently explanted and cultured in serum from untreated individuals for evaluation.
Embryo culture as above followed by organ culture. The prolongation of development in vitro could permit repair of damaged tissue or reveal abnormalities which appear late in gestation.
Key wordsEmbryo culture Teratogenic mechanisms
- Chatot CL, Klein NW, Piatek J, Pierro LT (1980). Successful culture of rat embryos on human serum: Use in the detection of teratogens. Science 207: 1471–1473.Google Scholar
- Kochhar DM (1981). Embryo explants and organ cultures in screening of chemicals for teratogenic effects. In Kimmel CA, Buelke-Sam J (eds): “Developmental Toxicology”, New York: Raven Press, pp 303–319.Google Scholar