Mutagenic Metabolites in Rat Urine After Dermal Exposure to 1,3-Diaminobenzene
Amberlite XAD-2 purified urine extracts from rats treated dermally with 1,3-diaminobenzene were fractionated by HPLC and the metabolites were identified by GC-MS. Mutagenic activity in Salmonella typhimurium TA 98 was equally distributed between the two fractions, none of which contained the parent amine.
The first fraction, eluted between 2–7 minutes, contained only a very small amount of presently unidentified metabolites, while the second fraction eluted between 14–19 minutes contained N,N’-diacetyl-1,3-diaminobenzene. Both fractions showed minor, but still significant activity even without metabolic activation.
A non-mutagenic fraction, eluted between 10–12 minutes contained Nacetyl-1,3-diaminobenzene and N,N’-diacetyl-2,4-diaminophenol.
Key words1,3-diaminobenzene Mutagenicity Metabolism Skin absorption.
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