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Mutagenic Metabolites in Rat Urine After Dermal Exposure to 1,3-Diaminobenzene

  • S. Clemmensen
  • H. Lam
  • A. Büchert
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 8)

Abstract

Amberlite XAD-2 purified urine extracts from rats treated dermally with 1,3-diaminobenzene were fractionated by HPLC and the metabolites were identified by GC-MS. Mutagenic activity in Salmonella typhimurium TA 98 was equally distributed between the two fractions, none of which contained the parent amine.

The first fraction, eluted between 2–7 minutes, contained only a very small amount of presently unidentified metabolites, while the second fraction eluted between 14–19 minutes contained N,N’-diacetyl-1,3-diaminobenzene. Both fractions showed minor, but still significant activity even without metabolic activation.

A non-mutagenic fraction, eluted between 10–12 minutes contained Nacetyl-1,3-diaminobenzene and N,N’-diacetyl-2,4-diaminophenol.

Key words

1,3-diaminobenzene Mutagenicity Metabolism Skin absorption. 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • S. Clemmensen
    • 1
  • H. Lam
    • 1
  • A. Büchert
    • 2
  1. 1.National Food InstituteSøborgDenmark
  2. 2.Institute of Toxicology and Central LaboratorySøborgDenmark

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