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The Technique of Gastric Endoscopy and Biopsy in the Dog

  • S. M. Whitehead
  • G. P. Crean
  • G. B. Leslie
  • D. Wild
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 8)

Abstract

The technique of gastric examination and biopsy using a fibre optic endoscope in the dog is described. This technique has been used on eight occasions to examine the stomachs of thirteen dogs as part of the investigations in a long-term toxicity study. The technique enables repeated examinations of the oesophagus and stomach to be made without any adverse effects on the stomach. The progress or resolution of lesions may be followed. The dogs have their food and bedding removed 12 hours before the procedure is due to begin. They are premedicated with acetylpromazine and atropine sulphate, and anaesthesied with intravenous sodium thiopentone. An endotracheal tube is passed and tied to the lower jaw. The endoscope is passed down the oesophagus and into the stomach. The endoscope has facilities for inflating the stomach and washing off and removing secretions by suction. Its tip can be reflected in two planes facilitating good visualisation of all areas of the stomach 2 mm biopsies of the gastric mucosa are taken using flexible biopsy forceps which are passed through a channel in the endoscope. Multiple biopsies are taken from the antrum, the greater curvature, and the lesser curvature of the stomach. They are placed on fibre-free absorbant card with mucosal surfaces uppermost for fixation in formol sublimate and subsequent processing.

Key words

Endoscopy Dog, gastric examination Gastric biopsy Mucosa 

References

  1. Crean GP, Leslie GB, Walker TF, Whitehead SM, and Roe FJC (1981) Safety Evaluation of Cimetidine; 54 month interim report on long-term study in dogs. Journal of Applied Toxicology. Vol 1, No 3Google Scholar
  2. Whitehead SM and Leslie GB (1983) Endoscopie examination of cimetidine-treated dogs. Proceedings of the SIR International Symposium. “Current Problems in Drug Toxicity”, 234–235. John Libbey Eurotext, London and Paris. Edited by G Zbinden, F Cohadon, JY Detaille, G MazueGoogle Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • S. M. Whitehead
    • 1
  • G. P. Crean
    • 2
  • G. B. Leslie
    • 1
  • D. Wild
    • 3
  1. 1.Department of ToxicologySmith Kline & French Research Ltd.Welwyn, HertsUK
  2. 2.Southern General HospitalGlasgowUK
  3. 3.Barr and Stroud Ltd.Anniesland, GlasgowUK

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