Ecto-ATPase Activity in Rat and Mouse Thymocytes and Cytotoxic Effects of Various Chemical Substances in Vitro

  • G. Hennighausen
  • E.-M. Hehl
  • J. Rychly
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 8)

Abstract

Ecto-ATPase was present in thymocytes of mice and rats with higher activity in rat thymocytes. In rat but not in mouse thymocytes the ecto-ATPase was inhibited in vitro by HgC12, tricyclohexyltin chloride, di-n-butyltin dichloride, DDT, γHCH and desipramine. The EC50 values ranged from 8 × 10−7 mol/1 for HgC12 to 4 × 10−4 mol/1 for desipramine. EctoATPase activity in thymocytes was not a valuable indicator of the cytotoxic effects of chemicals in vitro. Inhibition of the enzyme was not accompanied by changes of the electrophoretic mobility of thymocytes. A decrease of the spontaneous mitotic activity of thymocytes in vitro is a very sensitive parameter of cytotoxic and cytostatic drug effects.

Key words

Ecto-ATPase Thymocytes Cytotoxicity In vitro-cell electrophoresis Metal salts Organotin compounds γ HCH DDT Desipramine 

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References

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • G. Hennighausen
    • 1
  • E.-M. Hehl
    • 1
  • J. Rychly
    • 2
  1. 1.Institute of Pharmacology and ToxicologyWilhelm-Pieck-University of RostockRostockGDR
  2. 2.Clinic of Internal MedicineWilhelm-Pieck-University of RostockRostockGDR

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