Ecto-ATPase Activity in Rat and Mouse Thymocytes and Cytotoxic Effects of Various Chemical Substances in Vitro
Ecto-ATPase was present in thymocytes of mice and rats with higher activity in rat thymocytes. In rat but not in mouse thymocytes the ecto-ATPase was inhibited in vitro by HgC12, tricyclohexyltin chloride, di-n-butyltin dichloride, DDT, γHCH and desipramine. The EC50 values ranged from 8 × 10−7 mol/1 for HgC12 to 4 × 10−4 mol/1 for desipramine. EctoATPase activity in thymocytes was not a valuable indicator of the cytotoxic effects of chemicals in vitro. Inhibition of the enzyme was not accompanied by changes of the electrophoretic mobility of thymocytes. A decrease of the spontaneous mitotic activity of thymocytes in vitro is a very sensitive parameter of cytotoxic and cytostatic drug effects.
Key wordsEcto-ATPase Thymocytes Cytotoxicity In vitro-cell electrophoresis Metal salts Organotin compounds γ HCH DDT Desipramine
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