Experimental, Methodological and Analytical Approach to the Study of Microbe-Insecticide Interactions

  • Rup Lal
  • N. P. Agnihotri


The heterogenity of biological, chemical and physical properties of microbial environments has presented many problems to understand the microbe-insecticide interactions. In fact, many studies have examined such interactions only under laboratory conditions and thus the scientific literature on this subject is dominated by in vitro studies with pure and mixed cultures of microorganisms (Ware and Roan 1970; Cox 1972; Tu and Miles 1976; Wainright 1978; Lal and Saxena 1980, 1982; Lal 1982; Lal and Dhanaraj 1984). Such studies have considerable scientific value but there has been an unfortunate and undesirable tendency to assume that they also reflect the interaction of insecticides with microorganisms in natural environments.


High Pressure Liquid Chromatography Pesticide Residue Dansyl Chloride Pesticide Residue Analysis Organochlorine Insecticide 
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  1. Agarwal HC, Saxena DM, Lai R (1982) Accumulation and metabolism of DDT and its metabolites by Tetrahymena. Water Air Soil Pollut 18: 441–447CrossRefGoogle Scholar
  2. Aoki Y, Takeda M, Uchiyama M (1975) Comparative study of methods for the extraction of eleven organophosphorus pesticides residues in rice. J Assoc Off Anal Chem 58: 1286–1293PubMedGoogle Scholar
  3. Anderson JR, Davies PI (1973) Investigations on the extraction of adenosine triphosphate from soil. Bull Ecol Res Commun 17: 271–273Google Scholar
  4. Ault JA, Schofield CM, Johnson LD, Waltz RH (1979) Automated gel permeation chromatographic preparation of vegetables, fruits and crops for organophosphate residue determination utilizing flame photometric detector. J Agric Food Chem 27: 825–828PubMedCrossRefGoogle Scholar
  5. Ausmus BS (1973) The use of ATP in terrestrial decomposition studies. Bull Ecol Res Commun 17: 223–234Google Scholar
  6. Barry AL (1968) The accumulation of 14C-DDT by aquatic fungi. MSc thesis, Univ SalfordGoogle Scholar
  7. Bertuzzi PF, Kamp L, Miles CI (1967) Extraction of chlorinated pesticide residues from non- fatty samples of low moisture content. J Assoc Off Anal Chem 50: 623–626Google Scholar
  8. Beynon KS, Elgar KE (1966) The analysis for residues of chlorinated insecticides and acari-cides. Analyst 91: 143–175CrossRefGoogle Scholar
  9. Braun HE, Stanek J (1982) Application of the AO AC multi residue method to determination of synthetic pyrethroid residues in celery and animal products. J Assoc Off Anal Chem 65: 685–695PubMedGoogle Scholar
  10. Brown MJ (1975) Improved determination of residues of phorate and its principal metabolites. J Agric Food Chem 23: 334–335PubMedCrossRefGoogle Scholar
  11. Burke JA (1971) Development of Food and Drug Administration’s method of analysis for multiple residues of organochlorine pesticide residues in foods and feeds. Residue Rev 34: 59–90PubMedGoogle Scholar
  12. Burke JA, Porter ML, Young SJU (1971) Evaluation of two extraction procedures for pesticide residues resulting from foliar application and root absorption. J Assoc Off Anal Chem 54: 142–146PubMedGoogle Scholar
  13. Butler B, Hormann WD (1981) High pressure liquid chromatographic determination of cap- tan, captafol and fotpet residues in plant material. J Agric Food Chem 29: 257–260CrossRefGoogle Scholar
  14. Cox JL (1972) DDT residues in marine phytoplankton. Residue Rev 44: 23–28PubMedGoogle Scholar
  15. Dalton SA (1971) Uptake of 14C-DDT by river fungi and the effect of DDT on their growth and respiration. PhD thesis, Univ SalfordGoogle Scholar
  16. Doxtader KG (1969) Estimation of microbial biomass in the soil on the basis of adenosine triphosphate measurements. Semin 69th Annu Meet Am Soc Microbiol, Miami, USAGoogle Scholar
  17. Drobnik J (1955) Spiting der Strake durch den enzymatischen Komplex der Boden. Folia Biol (Prague) 1: 29Google Scholar
  18. Environmental Protection Agency USA (1974) Manual analytical methods for the analysis of pesticide residues in environmental samplesGoogle Scholar
  19. Faucheux LJ (1968) Rapid clean up for carbaryl, using thin layer chromatography. J Assoc Off Anal Chem 31: 676–678Google Scholar
  20. Goodwin ES, Goulden R, Raynolds JG (1961) Rapid identification and determination of residues of chlorinated pesticides in crops by gas-liquid chromatography. Analyst 86: 697–709CrossRefGoogle Scholar
  21. Goulding KH, Ellis W (1981) The interaction of DDT with two species of fresh water algae. Environ Pollut 25: 271–290CrossRefGoogle Scholar
  22. Gregory WW, John KR, Priester LE (1969) Accumulation of parathion and DDT by some algae and protozoa. J Protozool 16: 69–71PubMedGoogle Scholar
  23. Griffritt KR, Graun JC (1974) Gel permeation chromatographic system. An evaluation. J Assoc Of T Anal Chem 57: 168–172Google Scholar
  24. Gunner HB, Zuckerman BM (1968) Degradation of diazinon by synergistic microbial action. Nature (London) 217: 1183–1184CrossRefGoogle Scholar
  25. Gunther FA (1962) Instrumentation in pesticide residue determination. Adv Pest Control Res 5: 191–319Google Scholar
  26. Hank AR, Colvin BM (1981) High performance liquid chromatography. In: Das KG (ed) Pesticide analysis. Dekker, New York BaselGoogle Scholar
  27. Hansen PD (1979) Experiments on the accumulation of lindane (y-BHC) by the primary producer Chlorella Spec, and Chlorella pyrenoidosa. Arch Environ Contam Toxicol 62: 1437–1441Google Scholar
  28. Hardin LJ, Starter CT(1962) Comparison of fine extraction procedure for the recovery of DDT residues in field treated collard. J Assoc Off Anal Chem 45: 988–990Google Scholar
  29. Hofmann E, Schmidt W (1953) Über das Enzymsystem unserer Kulturbôden. 2 Urease. Bio- chem Z 324: 125–127Google Scholar
  30. Holden ER (1973) Gas chromatographic determination of residues of methyl carbamate insecticides in crop as their 2,4-dinitrophenyl ether derivatives. J Assoc Off Anal Chem 56: 713–717PubMedGoogle Scholar
  31. Hopper ML (1981) Gel permeation system for removal of fat during analysis of foods for residues of pesticide and herbicide. J Assoc Off Anal Chem 64: 720–723PubMedGoogle Scholar
  32. Johnen BG, Drew EA (1977) Ecological effects of pesticides on soil microorganisms. Soil Science 123: 319–324CrossRefGoogle Scholar
  33. Johnson LD, Waltz RH, Ussary JP, Kaiser FE (1976) Automated gel permeation chromatographic clean up of animal and plant extracts for pesticide residue determination. J Assoc Off Anal Chem 59: 174–196PubMedGoogle Scholar
  34. Lal R (1982) Accumulation, metabolism and effects of organophosphorus insecticides on microorganisms. Adv Appl Microbiol 28: 149–200PubMedCrossRefGoogle Scholar
  35. Lal R, Dhanaraj PS (1984) Effects of pesticides on microbial nitrogen fixation. Adv Agron 37 (to be published)Google Scholar
  36. Lal R, Saxena DM (1980) Cytological and biochemical effects of insecticides on microorganisms. Residue Rev 73: 49–86PubMedGoogle Scholar
  37. Lal R, Saxena DM (1982) Accumulation, metabolism and effects of insecticides on microorganisms. Microbiol Rev 46: 95–127PubMedGoogle Scholar
  38. Lai R, Saxena DM, Agarwal HC (1981) Uptake and metabolism of DDT by ciliate protozoan Stylonychia notophora. Acta Protozool 20: 109–114Google Scholar
  39. Lantos AAJ, Visi E, Csatlos I (1981) General methods for estimation of pesticide residues in sample of plant origin, soil and water. 1. Extraction and clean up. J Assoc Off Anal Chem 64: 783–792Google Scholar
  40. Lawrence JF, Turton DA (1978) High performance liquid chromatographic data for 166 pesticides. J Chromatogr 159: 207–226CrossRefGoogle Scholar
  41. Lawrence JF, Renault C, Frei RW (1976) Fluorogenic labeling of organophosphonate pesticides with dansyl chloride. J Chromatogr 121: 343–351PubMedCrossRefGoogle Scholar
  42. Lichtenstein EP (1965) In: Chichester LO (ed) Research in pesticides. Academic Press, London New York, pp 199–203Google Scholar
  43. Luke MA, Froberg JE, Doosi GM, Masumoto HT (1981) Improved multiresidue gas chromatographic determination of organophosphorus, organonitrogen and organohalogen pesticides in produce using flame photometric and electrolytic conductivity detectors. J Assoc Off Anal Chem 64: 1187–1195PubMedGoogle Scholar
  44. Matsumura F, Benezet J, Patil KC (1976) Factors affecting microbial metabolism of y-BHC. J PesticSci 1: 3–8CrossRefGoogle Scholar
  45. Mills PA, Oulers JH, Gather RA (1963) Rapid method for chlorinated pesticide residues in non fatty food. J Assoc Off Anal Chem 46: 186–191Google Scholar
  46. Mills PA, Boig BA, Kamps LR, Burke JA (1972) Elution solvent system for florisil column cleanup in organochlorine pesticide residue analysis. J Assoc Off Anal Chem 55: 39–43Google Scholar
  47. Moore-Landecker E, Stotzky CG (1972) Inhibition of the fungal growth and sporulation by volatile metabolites from bacteria. Can J Microbiol 18: 957–965PubMedCrossRefGoogle Scholar
  48. Munnecke DM, Johnson LM, Talbot HW, Barik S (1982) Microbial metabolism and enzymology of selected pesticides. In: Chakrabraty AM (ed) Biodégradation and detoxification of environmental pollutants. CRC Press, Boca Raton, Fla, pp 1–32Google Scholar
  49. Nitikin DI, Makarieva ED (1970) Use of electron microscope for estimation of microorganisms in soil suspensions. Sov Soil Sci 1970: 608–612Google Scholar
  50. Ohisa N, Yamaguchi M (1978) Degradation of gamma-BHC in flooded soil enriched with peptone. Agrie Biol Chem 42: 1983–1987CrossRefGoogle Scholar
  51. Ohisa N, Yamaguchi M, Kurihara N (1980) Lindane degradation by cell free extracts of Clos- terium rectum. Arch Environ Contam Toxicol 125: 221–225Google Scholar
  52. Porter ML, Burke JA (1969) Modification of AO AC method for multiple residues in no fatty food for samples of high sugar content. J Assoc Off Anal Chem 52: 1280–1283Google Scholar
  53. Porter ML, Gajan RJ, Burke JA (1969) Acetonitrile extraction and determination of carbaryl I fruits and vegetables. J Assoc Off Anal Chem 52: 177–181Google Scholar
  54. Ranteiner RA, Hormann WD (1975) Coupling high pressure liquid chromatography with cho linestrase inhibition Auto Analyser for determination of organophosphate and carbamate insecticides Residues. J Chromatogr 104: 438–442CrossRefGoogle Scholar
  55. Runkles JR, Scott AD, Nakayama FS (1958) Establishment of free living nitrogen-fixing bacteria in the rhizosphere and their effect on maize, tomato and wheat. Plant Soil 19:304–314Google Scholar
  56. Saxena DM, Lai R, Reddy BVP (1982) DDT uptake and metabolism in Blepharisma intermedium. Acta Protozool 21: 173–175Google Scholar
  57. Sethunathan N, Siddaramappa R, Rajaram KP, Barik S, Wahid PA (1977) Parathion residues in soil and water. Residue Rev 68:91—122 Skujins JJ (1967) In: Mclaren AD, Peterson GH (eds) Enzymes in soil. Dekker, New York, pp 371–414Google Scholar
  58. Skujins JJ (1967) In: Mclaren AD, Peterson GH (eds) Enzymes in soil. Dekker, New York, pp 371–414Google Scholar
  59. Storherr RW, Ott P, Watts RR (1971) A general method for organophosphorus pesticide residues in non-fatty foods. J Assoc Off Anal Chem 54: 513–524PubMedGoogle Scholar
  60. Stotzky G (1965) Microbial respiration. In: Black CA (ed) Methods of soil analysis, part 2. Chemical and microbiological properties. Am Soc Agron, Madison, pp 1550–1572Google Scholar
  61. Stotzky G (1972) Activity, ecology and population dynamics of microorganisms. Crit Rev Microbiol 2: 59–137CrossRefGoogle Scholar
  62. Suzuki T, Ischikawa K, Sato N, Sakai K (1979) Determination of chlorinated pesticide residues in foods II potassium permanganate oxidation for clean up of some vegetables. J Assoc Off Anal Chem 62: 685–688Google Scholar
  63. Tchan YT (1952) Counting soil algae by direct fluorescent microscopy. Nature (London) 170: 328–329CrossRefGoogle Scholar
  64. Tchan YT (1959) Study of soil algae III. Bioassay of soil fertility by algae. Plant Soil 10: 220–231CrossRefGoogle Scholar
  65. TU CM, Miles JRW (1976) Interaction between insecticides and soil microbes. Residue Rev 64: 17–66PubMedGoogle Scholar
  66. Wainright M (1978) A review of the effects of pesticides on microbial activity in soil. J Soil Sci 29: 287–298CrossRefGoogle Scholar
  67. Ware CW, Roan CC (1970) Interaction of pesticides with aquatic microorganisms and plankton. Residue Rev 33: 15–45PubMedGoogle Scholar
  68. Wheeler WB, Frear DEH, Mumma RO, Hamilton RH, Cotner RC (1967) Quantitative extraction of root absorbed dieldrin from the aerial parts of forage crops. J Agric Food Chem 15: 227–230CrossRefGoogle Scholar
  69. Yamato Y, Suzuki M, Watanbe T (1976) Organochlorine insecticides on mixed phase systems, II OV-l/OV-25, OV-210/OV-25 and OV-225/OV-25 systems. J Assoc Off Anal Chem 59: 1180–1183Google Scholar

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© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • Rup Lal
  • N. P. Agnihotri

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